The adherence and dissociation of Candida albicans, C. tropicalis, Streptococcus mutans and S. sanguis to six substrates including hydroxylapatite (HAP) which exhibit various hydrophobicity, was examined by the use of a bioluminescent adenosine triphosphate (ATP) assay. Dissolution of HAP by C. albicans or S. mutans was determined spectrophotometrically by the use of o-cresolphthalein complexone. In the adherence of C. tropicalis, S. mutans and S. sanguis, the amount of adherent cells correlated with the hydrophobicity of the substrates. In contrast, the adherence of C. albicans to HAP was extraordinary high, although the adherence of the fungi also correlated with the hydrophobicity of the substrates, except for HAP. The yeasts attached to HAP was effectively removed by high concentration of either phosphate or calcium ions. The amount of calcium-release from HAP caused by C. albicans and S. mutans was 113 microg ml(-1) (final pH = 3.45), and 5.4 microg ml(-1) (final pH 4.81), respectively and the maximum growth of C. albicans and S. mutans was 10(7) cfu ml(-1) and 7.4 x 10(12) cfu ml(-1), respectively. The results, taken together, suggest that C. albicans adhere to HAP specifically through electrostatic interaction, and that, in a much smaller number (1.0/7.4 x 10(5)), C. albicans possesses the ability to dissolve HAP to a greater extent (approximately 20-fold) when compared with S. mutans.
A sample of 308 extracted human permanent maxillary first molars from an Indonesian population was randomly selected. A rhomboid access cavity was made in all teeth in anticipation of identifying a second mesiobuccal canal (MB2). Ultrasonic tips were used to open the subpulpal groove to locate the second canal in the mesiobuccal root. Dentin was carefully removed from the trifurcation area at the expense of the mesial wall. If a second canal was located, a 0.8 C+ file was inserted into it until the file reached the apex. The prevalence of a second canal in the mesiobuccal root of the permanent maxillary first molar was 68.5% (95% CI: 63.1%-73.4%) in this population. Sections of the mesial root showed that the MB2 was a separate canal in 52.6% of the sample and a joined canal in 47.4%. The mean (SD) distance between MB1 and MB2 was 1.55 (0.66) mm. The data obtained from this study provide theoretical and experimental evidence to aid in the clinical management of the MB2 canal and may increase the success rate for root canal treatment of the maxillary first molar.
Background: In saliva, neutrophil constitutes the most prominent first-line defense of immune cells against pathogenic microbes. The importance of neutrophils to the host immune systems of neutropenic or patients disabled with regard to their neutrophil function results in a tendency toward serious infections, such as early childhood caries (ECC). The cytoplasmic granules present in neutrophils play a major role in neutrophil-mediated inflammation. Azurophilic granules contain antimicrobial proteins, such as defensin, a human antimicrobial peptide (HNP 1–3). The aim of this study is to analyze the correlation of HNP 1–3 secretion with CD63 expression on the surface of salivary neutrophils. Materials and Methods: This study constituted a cross-sectional, analytical observational study. Saliva taken from preschoolchildren between the ages of 4–6 years who had been divided into two groups, i.e., early childhood caries group with decayed, extracted, filled teeth (def-t) index >6 and caries free with def-t = 0, was subjected to a HNP 1–3 secretion test using ELISA assay and an expression test for CD63 by means of a flow cytometry test. The results obtained were analyzed using independent t -test and Pearson correlation ( P < 0.05). Results: The secretion of HNP 1–3 in the saliva of ECC was higher (172.6 ± 41.64) compared to that of caries-free cases (140.39 ± 31.91), whereas the level of CD63 salivary expression in ECC was lower (2.32 ± 0.57) than in the presence of caries (2.67 ± 0.46). Conclusion: In ECC cases, saliva increases HNP 1–3 secretion but decreases CD63 expression on the surface of salivary neutrophils.
BACKGROUND:The coastal region is the largest region in Indonesia as a country of the archipelago. Characteristics of groundwater content in coastal areas are very influential on dental health, especially dental caries. The main elements contained in 1-1000 mg/litre groundwater are calcium, magnesium, sodium, potassium, chlorine, bicarbonate, and sulfate groups. Calcium is an essential ingredient for living organisms that play a role in the formation of bone and tooth along with permeability of cell walls.AIM:This study aimed to analyse the relationship between calcium in groundwater with dental caries.METHOD:Analytical observational study with cross-sectional approach was implemented was coastal communities in Watu Ulo Jember Regency in February 2018 (3,686 inhabitants), with sample criteria of the minimum age of 12 years and consumed groundwater as drinking water at least 2 years by purposive side. The variables in this study were calcium levels as the independent variable and dental caries as the dependent variable. Calcium was measured using the spectrophotometric method. Caries measurements were performed using the DMF-T index. Data were presented descriptively in the table and analysed by Spearman Correlation test to analyze the relationship between groundwater calcium with dental caries.RESULTS:Average groundwater calcium content was 126.75 mg/litre (high category), and average dental caries was 2.2 (low category). Spearman correlation analysis showed p = 0.029 (p < 0.000), which means there was a correlation between groundwater calcium level with dental caries.CONCLUSION:There is a positive relationship between the calcium content of groundwater with dental caries.
Background: Oral health surveys conducted on a very large population involve many examiners who must be consistent in scoring different levels of an oral disease. Prior to the oral health survey implementation, a measurement of inter-rater reliability (IRR) is needed to know the level of agreement among examiners or raters. Purpose: This study aimed to assess the IRR using consensus and consistency estimates in large population oral health surveys. Methods: A total of 58 dentists participated as raters. The benchmarker showed the clinical sample for dental caries and community periodontal index (CPI) score, with the raters being trained to carry out a calibration exercise in dental phantom. The consensus estimate was measured by means of a percent agreement and Cohen’s Kappa statistic. The consistency estimate of IRR was measured by Cronbach’s alpha coefficient and intraclass correlation. Results: The percent agreement is 65.50% for photographic slides of dental caries, 73.13% for photographic slides of CPI and 78.78% for calibration of dental caries using phantom. There were statistically significant differences between dental caries calibration using photographic slides and phantom (p<0.000), while the consistency of IRR between multiple raters is strong (Cronbrach’s Alpha: >0.9). Conclusion: A percent agreement across multiple raters is acceptable for the diagnosis of dental caries. Consistency between multiple raters is reliable when diagnosing dental caries and CPI.
Background: Oral health is important for general health and quality of life. One of the oral diseases with a high prevalence in Indonesia is dental caries. Dental caries can cause limiting disturbances of daily activities such as biting, chewing, smiling and talking, and of psychosocial well-being, including development and general health of children. Purpose: This study aims to analyse the correlation of dental caries incidence rate with general health conditions and daily performance of children aged 2–5 years. Methods: This was an analytical observational cross-sectional study. The study sample was 103 pairs of children and their mothers, selected using cluster random sampling technique. Intra-oral examination was conducted on the children to obtain decay, missing, filled-teeth (DMF-T) index score. Information about oral impacts on daily performance (OIDP) of the children was collected through a questionnaire distributed to the mothers. The data obtained were statistically analysed with a regression test (p < 0.05). Results: It was found that dental caries had a significant correlation with general health (p = 0.00) and daily performance, including chewing function disorder (p = 0.00), difficulties in maintaining oral health (p = 0.039), sleep disorders (p = 0.00), and emotional instability (p = 0.00). Conclusion: The incidence rate of dental caries has a significant effect on the general health conditions and daily performance of children aged 2–5 years.
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