Characteristic of bovine hydroxyapatite-gelatin-chitosan scaffolds as biomaterial candidate for bone tissue engineering

Kartikasari, Nadia; Yuliati, Anita; Listiana, Indah; Setijanto, Darmawan; Suardita, Ketut; Maretaningtyas,; Ariani, Dwi; Sosiawan, Agung

doi:10.1109/iecbes.2016.7843524

Cited by 9 publications

(10 citation statements)

References 7 publications

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“…Gelatin is derived from collagen [ 48 ] by breaking the structure of the triple-helix into a single strand [ 49 ]. This material is biocompatible as well as biodegradable because of its amino acids (such as arginine-glycine-aspartic acid), which also encourage cell adhesion, migration, differentiation and proliferation [ 48 ]. Although gelatin comes from collagen, its antigenicity is lower, but there are still some information signals [ 50 ].…”

Section: Methodsmentioning

confidence: 99%

“…In bone-tissue engineering, it can be used alone or with other polymers or ceramics [ 52 ]. Chitosan has the same structure as a non-collagen organic component of ECM glycosaminoglycan (GAG) [ 48 ]. This is a linear polysaccharide consisting of D -glucosamine and N -acetyl- D -glucosamine linked by β (1-4) glycosidic bond [ 47 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

“…The degree of deacetylation affects the crystallinity of chitosan, and 100% deacetylated chitosan is highly crystalline [ 55 ]. Chitosan might support cell attachment, differentiation, and migration [ 48 ], and because of its non-toxicity, non-allergenicity, mucoadhesivity, biocompatibility, biodegradability and osteoconductivity, it can be applied as a dental, bone or cartilage implant, or as artificial skin. Cross-linked chitosan can be used as a bandage.…”

Section: Methodsmentioning

confidence: 99%

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Fabrication of Scaffolds for Bone-Tissue Regeneration

2019

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The present article describes the state of the art in the rapidly developing field of bone tissue engineering, where many disciplines, such as material science, mechanical engineering, clinical medicine and genetics, are interconnected. The main objective is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair. Strategies and materials used in oral regenerative therapies correspond to techniques generally used in bone tissue engineering. Researchers are focusing on developing and improving new materials to imitate the native biological neighborhood as authentically as possible. The most promising is a combination of cells and matrices (scaffolds) that can be fabricated from different kinds of materials. This review summarizes currently available materials and manufacturing technologies of scaffolds for bone-tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Fabrication of Scaffolds for Bone-Tissue Regeneration

2019

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“…Besides, there was no wide agglomeration of these nanoparticles showing the uniform distribution of particles. It was also observed that the microscopic structure of the fracture surface morphology for most of the nanoparticles are embedded inside the matrix material as an integral part of the base material structure, indicating good compatibility between the matrix material and leads to the formation of strong physical bonding (strong interfacial regions) between the nanoparticles and polymeric blending matrix [3,23]. It was also observed that the microscopic structure of the fracture surface morphology for most of the nanoparticles are embedded inside the matrix material as an integral part of the base material structure, indicating good compatibility between the matrix material, and lead to the formation of strong physical bonding (strong interfacial regions) between the nanoparticles and polymeric blending matrix [3,23].…”

Section: Resultsmentioning

confidence: 99%

“…Systems for bone tissue engineering include bone regeneration following tissue loss owing to degenerative surgical procedures. In the condition of disease with degeneration risk, the therapy requires the surgical removal of the tumor bone tissue and the bone defect can be filled with bone graft material in the second phase [3]. Polymethyl methacrylate (PMMA) is a non-adhesive acrylic polymer, widely used as bone cement for implants in orthopedics.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Polymer Blend and Nano Composite Materials Based on PMMA Used for Bone Tissue Regeneration

Alsaedi¹,

Salih

Hashim

2020

ETJ

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As the elderly population increases, the need for bone loss treatments is increasing. Vital substances used in such treatments are required to continue for a longer period and work more effectively. The particularly important biological material is poly methyl methacrylate (PMMA) bone cement, which is widely used in damaged bone replacement surgery. So, this study focused on the role of added some nanoparticles consist of zirconia (ZrO2), and magnesia (MgO) on the binary polymeric blend (Acrylic bone cement: 15% PMMA) for a bone scaffold. Where, ZrO2 and MgO nanoparticle was added with selected weight percentages (0, 0.5, 1, 1.5 and 2 wt.%), which were added to the polymer blend matrix. Some mechanical properties were studied including the tensile strength and young modulus for all the prepared samples. The chemical bonding of nanoparticles and synthetic binary polymeric blend composites was evaluated by Fourier Transform Infrared (FTIR) spectroscopy. Tensile strength and young modulus of binary polymeric blend reinforced with 1.5 wt.% ZrO2, and 1 wt.% MgO, significantly increased. The surface morphology of the fracture surface of tensile specimens was examined by Scanning electron microscope (SEM). The SEM images confirmed that the homogenous distribution of nanoparticles (ZrO2, and MgO) within the polymeric blend matrix.

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Bifunctional tissue‐engineered composite construct for bone regeneration: The role of copper and fibrin

Bozorgi,

Khazaei,

Bozorgi

et al. 2023

J Biomed Mater Res

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Bifunctional tissue engineering constructs promoting osteogenesis and angiogenesis are essential for bone regeneration. Metal ion‐incorporated scaffolds and fibrin encapsulation attract much attention due to low cost, nontoxicity, and tunable control over ion and growth factor release. Herein, we investigated the effect of Cu.nHA/Cs/Gel scaffold and fibrin encapsulation on osteogenic and angiogenic differentiation of Wharton's jelly mesenchymal stem cells (WJMSCs) in vitro and in vivo. Cu‐laden scaffolds were synthesized using salt leaching/freeze drying and were characterized using standard techniques. WJMSCs were isolated from the human umbilical cord and characterized. WJMSCs with or without encapsulating in fibrin were seeded onto scaffolds, followed by differentiating into the osteogenic lineage for 7 and 21 days. Osteogenic and angiogenic differentiation were evaluated using real‐time polymerase chain reaction, western blot, and Alizarin red staining. Then, scaffolds were implanted into critical‐sized calvarial bone defects in rats and histological assessments were performed using hematoxylin/eosin, Masson's trichrome, and CD31 immunohistochemical staining at 4 and 12 weeks. The scaffolds had good physicochemical and biological characteristics suitable for cell attachment and growth. Cu and fibrin increased the expression of ALP, RUNX2, OCN, COLI, VEGF, and HIF1α in differentiated WJMSCs. Implanted scaffolds were also biocompatible and were integrated well with the host tissue. Increased collagen condensation, mineralization, and blood vessel formation were observed in Cu‐laden scaffolds. The fibrin‐encapsulated groups showed the highest collagen and cell densities, immune cell infiltration, and bone trabeculae. CD31‐positive cell population increased with fibrin encapsulation and seeding onto Cu‐laden scaffolds. Adding Cu to scaffolds and encapsulating cells in fibrin are promising methods that guide osteogenesis and angiogenesis cellular signaling, leading to better bone regeneration.

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Characteristic of bovine hydroxyapatite-gelatin-chitosan scaffolds as biomaterial candidate for bone tissue engineering

Cited by 9 publications

References 7 publications

Fabrication of Scaffolds for Bone-Tissue Regeneration

Fabrication of Scaffolds for Bone-Tissue Regeneration

Preparation and Characterization of Polymer Blend and Nano Composite Materials Based on PMMA Used for Bone Tissue Regeneration

Bifunctional tissue‐engineered composite construct for bone regeneration: The role of copper and fibrin

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