Phytochemical investigation of the gum resin of Ferula assafoetida resulted in the isolation and characterization of a new sesquiterpenoid coumarin, Saradaferin (1) named as [Decahydro-(3-alpha-hydroxy-4,4,10-trimethyl-8-methylene-9-naphthenyl)-alpha-hydroxymethyl] ether of umbelliferone.
The structure and stereochemistry of a new terpenoid ester, nardostachysin (1), isolated from the rhizomes of Nardostachys jatamansi, were established as the 7',8'-dihydroxy-4'-methylene hexahydrocyclopenta[c]pyran-1'-one-8'-methyl ester of 7, 9-guaiadien-14-oic acid, by spectral and chemical studies.
Terpenes U 0200 Studies on the Chemical Constituents of Nardostachys jatamansi DC (Valerianaceae). -[isolation and structure determination of the new sesquiterpene acid, nardin (I), and the new pyranocoumarin (II)] -(CHATTERJEE*, A.; BASAK, B.; DATTA, U.; BANERJI, J.; NEUMAN, A.; PRANGE, T.; Indian J. Chem., Sect. B: Org. Chem. Incl. Med. Chem. 44 (2005) 2, 430-433; Cent. Adv. Stud. Nat. Prod., Univ. Coll. Sci., Calcutta 700 009, India; Eng.) -A. Forchert 25-180
Studies have been carried out on the protein solubility profile of Kulthi (Macrotylona uniflorus, Lam.) seed in aqueous solution over various pHs and at different concentrations of NaCl, Na2SO3, CaCl2, and MgCl2 at pH 8.0. Amino acid analysis of isolated protein identified 17 amino acids, 9 of which are essential. Gel-permeation chromatography on Sephadex G-200 revealed the presence of seven components in the protein fraction. Their molecular weights were determined by two comparable standard methods. Extractable Kulthi seed proteins in salt solutions were separated electrophoretically into eight fractions whose molecular weights were found to be 186,200, 131,800, 108,400, 91,200, 53,700, 44,700, 38,000, and 27,500.
Proteins were extracted from the deoiled seeds ofTectona grandis Linn., Fam. Verbenaceae, a quality lumber source, in aqueous solutions of various pH's or by different concentrations of NaCl at pH 8.0. Chemical analysis of isolated protein identified 15 amino acids, of which eight were essential. Gel filtration on Sephadex G‐200 revealed the presence of six components, whose molecular weights were determined by two comparable standard methods. Seven components were resolved electrophoretically (SDS‐PAG electrophoresis) and their molecular weights were found to be 118,900, 92,300, 72,400, 62,400, 43,600, 39,800 and 32,400.
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