Isolation and chemical investigation of teak (Tectona grandis linn) seed proteins

Abstract

Proteins were extracted from the deoiled seeds ofTectona grandis Linn., Fam. Verbenaceae, a quality lumber source, in aqueous solutions of various pH's or by different concentrations of NaCl at pH 8.0. Chemical analysis of isolated protein identified 15 amino acids, of which eight were essential. Gel filtration on Sephadex G‐200 revealed the presence of six components, whose molecular weights were determined by two comparable standard methods. Seven components were resolved electrophoretically (SDS‐PAG electrophoresis) and their molecular weights were found to be 118,900, 92,300, 72,400, 62,400, 43,600, 39,800 and 32,400.

Proximate Composition and Amino Acid Analysis of Defatted Tectona Grandis Seed Meal

“…Chemical analysis of isolated protein identified 18 amino acids of which nine were essential and nine were nonessential. This is in disagreement with a similar investigation of Laskar et al, (1985) who identified 15 amino acids of which eight were essential and seven were non-essential. Leucine had the highest value of essential amino acid with a total 23.3% of the total value and closely followed by phenylalanine with 18.5% of the total essential amino acid.…”

“…Since the seed meal is rich in these essential amino acids, it could probably be a source for both livestock and human beings while the others not available enough could be supplemented. Methionine and tryptophan had the lowest amount of essential amino acid with 0.899 and 0.806 g/100g representing 3.11 and 2.80% of the total essential amino acids respectively but these could not be detected by Laskar et al, (1985). Since the samples were taken from different locations far away from each other, different climatic or environmental conditions could have been responsible for the difference.…”

Some chemical investigations of Amoora rohituka seed proteins

“…The protein solubility profile of the de-oiled seed flour was determined under two different conditions (Basak, Bhattacharya, Sinhababu, & Laskar, 1994;Guerra & Park, 1975;Laskar, Ghosh Majumder, & Basak, 1985;Laskar, Sinhababu, Thakur, & Basak, 1998;Laskar, Thakur, & Basak, 1988). In case I, extraction was carried out with an electrical stirrer at room temperature for 30 min using deionised distilled water to de-oiled seed flour (20:1, w/v) at pHs 2-12.…”

“…The pH of extraction was adjusted throughout the experiment to 2-6 by adding 0.5 M hydrochloric acid or to pH 7-12 by adding 0.5 M sodium hydroxide. In case II, the solubility profile of the seed protein was determined at a fixed pH (7.0) using various concentrations (0.1-1.0 M) of NaCl, KCl, CaCl 2 and MgSO 4 (Basak et al, 1994;Guerra & Park, 1975;Laskar et al, 1985;Laskar, Sinhababu, et al, 1988;Laskar, Thakur, et al, 1998). Respective pHs in both the cases were maintained throughout the experiment.…”

Fertility Variation and Effective Population Size in A Teak Clonal Seed Orchard

“…Teak (Tectona grandis L.f.) is a native species in Southeast Asia, and distributes naturally only in the Indian Peninsula, Myanmar, Northern Thailand and Northwestern Laos along the northern Thai border (Troup, 1921), and Burma (Laskar, et al, 1985). It grows at latitude 9 o S (in Myanmar) to 25 o N (in India), and longitude 70º -100º E (Rachmawati, et al, 2002).…”