Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates

Laskar, Subrata; Bhattacharya, Utpal; Basak, Bidyut

doi:10.1039/an9911600625

Cited by 23 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is very difficult to visualize the amino acids by the UV lamp method and considerable efforts have been made to detect them on the TLC surface by the dyeing reagent [2][3][4] or coupling TLC with other techniques such as MS . We have applied the TLC-IMS method for a mixture of the amino acids alanine and isoleucine.…”

Section: Analysis Of the Mixtures Of Amino Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Ilbeigi

Sabo

Valadbeigi

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

Section: Analysis Of the Mixtures Of Amino Acidsmentioning

confidence: 99%

“…Traditionally, the analyte spots on the TLC plate are visualized by chemical and optical methods such as staining techniques and dyeing reagents [2][3][4] ultraviolet (UV) absorption and fluorescence spectroscopy [5].…”

Section: Introductionmentioning

confidence: 99%

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Ilbeigi

Sabo

Valadbeigi

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

“…The detection or identification of amino acids is extremely important in biomedical and biochemical analysis for the evaluation of protein structure as the amino acids are the monomeric units of proteins; these amino acids are used by cells for protein biosynthesis, and also exist in the free state in numerous natural products (seeds and leaves) and as the C-terminal determination of degraded proteins. Several specific and non-specific reagents have been reported on thin-layer chromatography (TLC) plates [5][6][7][8][9][10]. Such identification is the most well-known reagent is ninhydrin which is widely used for its remarkable high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Separation of Amino Acids Based on Thin-Layer Chromatography by a Novel Quinazoline Based Anti-Microbial Agent

Sen¹,

Sarkar²,

Kundu³

et al. 2012

AJAC

Self Cite

View full text Add to dashboard Cite

A newly designed quinazoline based compound, 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline (PDBIQ) has shown the ability for the easy detection of nineteen amino acids on thin-layer chromatography plates as a spray reagent. This new reagent enabled to produce various distinguishable colors with amino acids with different R<sub>F</sub> values. The detection limits and the binding ability of PDBIQ with amino acids have been calculated. PDBIQ is also able to detect aminoacids from hydrolised seed protein. The title compound also exhibited profound inhibitory action against some gm (+ve) and gm (-ve) bacterial organisms. This paper deals with synthesis, spectroscopic application and biological evaluation of the organic moity

show abstract

“…Another possibility for detection of amino acids was also described (Devani et al 1991;Laskar et al 1991;Laskar and Basak 1990a, b;Sinhababu et al 1994). TLC analysis of amino acids extracted from various plants were described (Gaspar and Males 2005;Males et al 2001Males et al , 2004Males et al , 2005Kopyt'ko 2003).…”

Section: Detection and Identification Of Biomolecules In Tlcmentioning

confidence: 98%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

View full text Add to dashboard Cite

Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

show abstract

Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates

Cited by 23 publications

References 3 publications

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Separation of Amino Acids Based on Thin-Layer Chromatography by a Novel Quinazoline Based Anti-Microbial Agent

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Contact Info

Product

Resources

About