This study aims to determine the effect of different priming methods on the viability and early growth of upland rice under drought stress. Priming is an important approach to improve the resistance of upland rice plants to drought stress from the germination phase to growth, especially on sub-optimal land. There are several efficient priming methods as seed pretreatment to increase germination and tolerance to drought stress. An effective priming method is needed to increase the germination and growth of upland rice seedlings for application in dryland agriculture. Therefore, an experiment was conducted at the Seed laboratory of Pangkep State Polytechnic of Agriculture, Indonesia, from September to October of 2022 to investigate the effect of several priming methods on the germination and growth of upland rice seedlings under drought stress. The experiment consisted of 2 stages, germination testing and seedling growth phase testing with the addition of water stress treatment. Experiment 1 was conducted in a completely randomized design (CRD) with four replications, including Control (no priming), Osmopriming with 15% and 20% PEG solution, Redox Priming with 3% and 6% H2O2 solution, and Organic Priming using 50% and 75% Moringa leaf extract. Experiment 2 was conducted using a two-factor of factorial in Randomized Block design (RBD) with three replications. The first factor is the Seedling results of stage 1 experiments (selected the best of each priming method) includes 4 treatments each Control treatment, Osmopriming with 15% PEG solution, Redox Priming with 3% H2O2 solution, and Organic Priming with 50% Moringa Leaf Extract. The second factor is the level of Drought Stress conducted by 100%, 60%, and 30% of Field Capacity. The results showed that the priming method with Osmopriming 15% PEG solution gave the best results on Seed germination percentage (87.5%) followed by Organic priming with 50% Moringa Leaf Extract (SGP 85%). Under drought stress conditions with 30% field capacity, the highest increase in proline levels was observed with H2O2 redox priming 3% (10.3 µ-mol. g-1 ), while the average root growth of all primed seedlings showed better root growth than seeds without priming treatment. Seed priming gives better results on the growth and physiological activities of upland rice at several levels of drought stress, in the early growth phase of seedlings
Utilization of Exopolisakarida Bacteria as adhesive agent and binder soil structure so that the structure of the soil becomes steady. EPS bacteria play a role in stabilizing soil aggregates through augmentation techniques in potato plant rhizosphere.This study aims to characterize the bacteria producing eksopolisakarida (EPS) obtained from the rizosphere of highland potato plants which are focussed as promoters of plant growth. There are 3 sample sampling locations that are distinguished by the height of the sea level: 1200, 1500 and 1800 m above sea level in in Malino, South Sulawesi. Soil samples were diluted and cultured on ATCC medium No. 14 and the specific medium of exopolisakarida-producing bacteria Mac Concay medium. After testing there were 15 EPS bacterial isolates that had the potential to produce exopolysaccharides and all the bacteria were grouped in gram-negative bacteria. Isolates P3T (63) yielded an IAA ( Indole Acetic Acid) value of 21.14 ppm and as a phosphate solvent of 20, 59 ppm. While Isolate P3T (50) has the ability to fix Nitrogen of 1, 42 ppm, and produce EPS of 1,07 mg/mg protein with the best carbon source for producing exopolysaccharides is Sucrose. Keywords : Exopolysaccharide, Promoters, IAA, Phosphate solvent, Nitrogen Fixation
The condition of cotton planting in South Sulawesi is always constrained in the fulfillment of water. All plant growth stages are not optimal to increase production, so it is necessary to introduce good water management technology, such as through water supply with drip irrigation system. This study aims to analyze the strategy of irrigation management in cotton plants using drip irrigation system. Model of application by designing drip irrigation system and cotton planting on land prepared as demonstration plot. Observations were made in the germination phase and the vegetative phase of the early plants. Based on the result of drip irrigation design, the emitter droplet rate (EDR) was 34.266 mm/hour with an operational time of 4.08 min/day. From the observation of cotton growth, it is known that germination time lasted from 6 to 13 days after planting, the average plant height reached 119.66 cm, with the number of leaves averaging 141.93 pieces and the number of bolls averaging 57.16 boll.
Bakteri eksopolisakarida (EPS) mempunyai peranan terhadap pertumbuhan tanaman karena berfungsi sebagai biofertilizer yaitu dapat memberikan kesuburan pada tanah karena dapat melarutkan posfat dan menfiksasi nitrogen selain itu bakteri EPS menghasilkan IAA, dan bakteri EPS digunakan sebagai bahan aktif pembuatan bahan pembenah tanah (Bioamelioran). Penelitian ini bertujuan untuk membandingkan pertumbuhan bibit kopi yang diberi perlakuan bahan pembenah tanah (bioamelioran) dan bahan organik pada berbagai konsentrasi. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan perlakuan berbagai dosis pemberian bahan pembenah tanah (bioamelioran) yaitu: kontrol (K0), 3 ml/l (K1), 4 ml/l (K2), 5 ml/l (K3). Setiap perlakuan terdiri dari 2 unit yang diulang sebanyak 3 kali sehingga terdapat 24 unit percobaan. Dosis bahan organik yang diberikan sebanyak 30 ton/ha atau setara dengan 53 gram/polybag, Jenis bibit kopi yang digunakan adalah kopi Liberika. Hasil percobaan menunjukkan bahwa pemberian bioamelioran dengan dosis 5 ml/l memperlihatkan pertumbuhan perkecambahan tercepat (21,17 hst), dengan tanaman tertinggi (18,92 cm), dan daun terbanyak (9 helai) terhadap perkecamabahan kopi. Berdasarkan hasil percobaan dapat disimpulkan bahwa pemberian bioamelioran (bahan pembenah tanah) dan bahan organik memberikan hasil terbaik pada perlakuan dengan konsentrasi 5 ml/liter air yaitu pada parameter kecepatan perkecambahan, tinggi tanaman, dan jumlah daun.
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