Effect of Different Priming Methods on Seed Viability and Seedling Growth of Upland Rice under Drought Stress

Abstract

This study aims to determine the effect of different priming methods on the viability and early growth of upland rice under drought stress. Priming is an important approach to improve the resistance of upland rice plants to drought stress from the germination phase to growth, especially on sub-optimal land. There are several efficient priming methods as seed pretreatment to increase germination and tolerance to drought stress. An effective priming method is needed to increase the germination and growth of upland rice seedlings for application in dryland agriculture. Therefore, an experiment was conducted at the Seed laboratory of Pangkep State Polytechnic of Agriculture, Indonesia, from September to October of 2022 to investigate the effect of several priming methods on the germination and growth of upland rice seedlings under drought stress. The experiment consisted of 2 stages, germination testing and seedling growth phase testing with the addition of water stress treatment. Experiment 1 was conducted in a completely randomized design (CRD) with four replications, including Control (no priming), Osmopriming with 15% and 20% PEG solution, Redox Priming with 3% and 6% H2O2 solution, and Organic Priming using 50% and 75% Moringa leaf extract. Experiment 2 was conducted using a two-factor of factorial in Randomized Block design (RBD) with three replications. The first factor is the Seedling results of stage 1 experiments (selected the best of each priming method) includes 4 treatments each Control treatment, Osmopriming with 15% PEG solution, Redox Priming with 3% H2O2 solution, and Organic Priming with 50% Moringa Leaf Extract. The second factor is the level of Drought Stress conducted by 100%, 60%, and 30% of Field Capacity. The results showed that the priming method with Osmopriming 15% PEG solution gave the best results on Seed germination percentage (87.5%) followed by Organic priming with 50% Moringa Leaf Extract (SGP 85%). Under drought stress conditions with 30% field capacity, the highest increase in proline levels was observed with H2O2 redox priming 3% (10.3 µ-mol. g-1 ), while the average root growth of all primed seedlings showed better root growth than seeds without priming treatment. Seed priming gives better results on the growth and physiological activities of upland rice at several levels of drought stress, in the early growth phase of seedlings

The application of seed priming agents in enhancing drought resilience of rice: a comprehensive review

“…Notably, MLE from the Islamabad landrace exhibited the most significant effects [39]. In different experiment, Kadir et al (2023) confirmed that using 50% MLE improved 85% seed germination percentage than non-primed rice. The primed seed also showed better growth and physiological activities of upland rice at several levels of drought stress, in the early growth phase of seedlings [37].…”

Improvement of Vigor and Early Growth of Two Composite Maize Varieties Through Seed Priming Treatment

“…Research by Kadir et al (2023) indicated that the different priming methods given to the seeds gave different results on upland rice seed viability and seedling growth under drought conditions. The priming method with osmopriming 15% PEG solution gave the best results on seed germination percentage (87.5%).…”

“…Priming enhances plant defense against biotic and abiotic stresses (Hossain et al, 2015). It is defined as the initial exposure of seeds or young seedlings to chemical or abiotic stresses that makes the plant more resistant to subsequent phases and better able to detect a second signal quickly (Kadir et al, 2023). Seed priming, a promising technique, has been successfully used to overcome the problems of poor germination and subsequent erratic plant establishment under normal and stressful conditions, and numerous studies have been conducted on seed priming to improve germination rate and uniformity of plant growth in numerous field crops, including maize.…”

“…Seed priming, a promising technique, has been successfully used to overcome the problems of poor germination and subsequent erratic plant establishment under normal and stressful conditions, and numerous studies have been conducted on seed priming to improve germination rate and uniformity of plant growth in numerous field crops, including maize. A series of bioassays involving various priming agents, such as CaCl2 (Farooq et al 2008), Mg (NO3)2 and ZnSO4 (Singhal, et al 2021), salicylic acid (Farooq et al 2007), and Moringa leaf extract (Kadir et al 2023) have been tested to improve maize performance under low-temperature stress.…”

Seed priming as a method of preservation and restoration of sunflower seeds

“…The most commonly used priming agents are PEG (Polyethylene glycol m.w. 6000 and 8000) (Arif et al, 2010;Kadir et al, 2023), which are non-toxic highmolecular-weight compounds, inorganic salts such as magnesium, potassium, and sodium (Sher et al, 2019) and lowmolecular-weight organic compounds such as glycerol, mannitol, and sucrose. In addition, plant growth regulators (PGR) such as gibberellic acid (GA 3 ) and ethylene are used alone or in combination (Kaya, 2008;Zhu et al, 2021;Zhang et al, 2023).…”