Karakterisasi Bakteri Penghasil Eksopolisakarida Asal Rhizosfer Tanaman Kentang Sebagai Promotor Pertumbuhan Tanaman

Abstract

Utilization of Exopolisakarida Bacteria as adhesive agent and binder soil structure so that the structure of the soil becomes steady. EPS bacteria play a role in stabilizing soil aggregates through augmentation techniques in potato plant rhizosphere.This study aims to characterize the bacteria producing eksopolisakarida (EPS) obtained from the rizosphere of highland potato plants which are focussed as promoters of plant growth. There are 3 sample sampling locations that are distinguished by the height of the sea level: 1200, 1500 and 1800 m above sea level in in Malino, South Sulawesi. Soil samples were diluted and cultured on ATCC medium No. 14 and the specific medium of exopolisakarida-producing bacteria Mac Concay medium. After testing there were 15 EPS bacterial isolates that had the potential to produce exopolysaccharides and all the bacteria were grouped in gram-negative bacteria. Isolates P3T (63) yielded an IAA ( Indole Acetic Acid) value of 21.14 ppm and as a phosphate solvent of 20, 59 ppm. While Isolate P3T (50) has the ability to fix Nitrogen of 1, 42 ppm, and produce EPS of 1,07 mg/mg protein with the best carbon source for producing exopolysaccharides is Sucrose. Keywords : Exopolysaccharide, Promoters, IAA, Phosphate solvent, Nitrogen Fixation

Eksplorasi Bakteri Penghasil Eksopolisakarida dari Rizosfer Kelapa Sawit sebagai Pemantap Agregat Tanah [Exploration of exopolysaccharide-producing bacteria from Oil Palm Rhizosphere as a Soil Aggregate stabilizer]

“…Eksopolisakarida dapat digunakan sebagai biosorben potensial untuk menghilangkan logam berat dari lingkungan (Shuhong et al, 2014), meningkatkan kemampuan tanah memegang air dan mentransformasi senyawa fungisida di dalam tanah (Yang et al, 2014). Mu'minah et al (2016) mendapat 3 isolat bakteri yang potensial menghasilkan eksopolisakarida yang diperoleh dari rhizosfer tanaman kentang pada kelerengan 15%, 25% dan 35% dari Malino, Kecamatan Tinggi, Kecamatan Moncong, Gowa, Sulawesi Selatan, Indonesia. Penelitian Harahap et al (2018) yang menguji 25 isolat potensial yang diisolasi dari tanah berpasir di Kalimantan Barat, mendapatkan 3 isolat unggul untukdikembangkan sebagai bakteri pemantap agregat, yaitu isolat RB292, RB51 dan RB241 yang menghasilkan bobot kering EPS berturut-turut sebesar 7,53; 7,55 dan 7,59 mg.mL -1 dengan sumber karbon sukrosa 2%.…”