Abstract. Sidhiq DF, Subositi D, Widiyastuti Y, Pujiasmanto B, Yunus A. 2020. Morphological diversity, total phenolic and flavonoid content of Echinacea purpurea cultivated in Karangpandan, Central Java, Indonesia. Biodiversitas 21: 1265-1271. Purple coneflower (Echinacea purpurea (L.) Moench) is a medicinal plant introduced from North America. While it has been introduced to Indonesia long several decades ago. E. purpurea has not been developed as a raw material for herbal medicines in Indonesia as it faces constraints, namely non-uniform production and lack of information on the total phenolic and flavonoid content if cultivated in the country. This study aims to determine the morphological diversity, total phenolic and flavonoid content of three accession E. purpurea cultivated on lowland area (i.e. 493 m asl) in Tegalgede, Karangpandan, Central Java, Indonesia. This study used a Randomized Complete Group Design (RCBD) method with 1 factor, namely 3 accessions of E. Purpurea: A1, A2, A3 each with four replications. Morphological observations, as well as laboratory analysis, were done in this research to collect quantitative and qualitative data. The quantitative data were analyzed using SPSS to test the difference. We found there is significantly different results E. Purpurea accession in term of plant height, wet stover weight, number of leaves, root weight, root length, number of branches. Quantitative observation in the form of extract yield, total phenol content and total flavonoid highest content of extract residue produced by Accession 3 with 7,655%, while the highest phenolic was produced by Accession 1 with 507,619 mg GAE.g -1 extract, and the highest flavonoid was produced by Accession 2 with 313,869 mg QE.g-1 extract. This study concludes that there is morphological diversity of E. purpurea cultivated in Karangpandan with a significant correlation of accession to residue extract, phenolic, and flavonoid content.

Corn silk is a part of the corn plant that is still considered to be waste from corn processing; however, corn silk is actually known to have the potential to be developed as functional food ingredients and/or nutraceuticals. The potential applications of corn silk are closely related to its chemical composition and the action mechanisms of its bioactive compounds, which have beneficial effects on human health, such as flavonoids and phenolics. This study aimed to investigate the chemical and phytochemical characteristics of local corn silk powder of three different varieties, i.e. Bisma, Arjuna, and Srikandi Putih. Phytochemical and proximate analyses were conducted on each sample of corn silk powder. The total phenolic content was examined using the Folin-Ciocalteau method, while the beta sitosterol was analysed by gas chromatography and the antioxidant activity of the extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results show that all samples had different amounts for their moisture, ash, protein, fat and carbohydrate contents from the proximate analyses. The Bisma variety of corn silk powder reveals the highest fat and protein contents, which are 0.30%±0.02 and 17.70 ±0.47, respectively. Furthermore, the highest total phenolics, total flavonoids, beta-sitosterol contents and antioxidant activity for the corn silk powder are also demonstrated by Bisma; i.e. 8262.93±178.59 μg gallic acid equivalent (GAE)/g for total phenolics, 236.03±8.37 μg gallic acid equivalent (GAE)/g for total flavonoids, 1343.93±78.44ppm for beta-sitosterol and 73%±1.09 for antioxidant activity. Thus, it may be suggested that among the three local varieties of corn silk, a corn silk powder from the Bisma variety could be developed as a source of bioactive compounds and nutrients to convert corn silk from being waste into value-added corn products.

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Cempo Ireng rice is local rice that is rich in anthocyanins. The issue with Cempo Ireng rice is its lengthy harvest life, high habitus, and low production. Plant mutations are one method for overcoming this. The study’s goal is to create better mutant plants with high yields. The study was conducted by placing three lines in plots from December 2019 to August 2020. The results were descriptively evaluated and compared to the control using the T-test. The results showed that the mutant plant was superior to black rice without irradiation (control). The line with the highest productivity value (per hectare) was line 51 which was 8.45 tons/ha. Line 44 has the highest average anthocyanin content of 75.10 ppm.

Corn silk from female flowers consists of a group of stigmas that are smooth, soft, and look like threads and yellowish silk. Corn silk is rich in phenolic compounds, especially flavonoids and high natural antioxidant compounds. This content varies according to the corn varieties. Bisma variety is one of the many local corn varieties grown in Temanggung, Central Java. The first step in separating phenolic compounds is extraction with solvents. Methanol is one of the solvents that are often used in the extraction of phenolic compounds. Some of the contributing factors affect the rate and quality of extraction on the components of bioactive compounds, two of which are temperature and length of extraction. This study aims to determine the optimum condition of extraction of corn silk from local Bisma varieties using Response Surface Methodology. The experimental design used was factorial in the Randomized Block Design (RBD) with two factors and three replications. The local corn silk of the Bisma variety, 80% methanol solvent concentration and a ratio (1:20) were extracted with a length of extraction (60, 70, 80 and 90) minutes and a temperature of 40° C, 50°C, 60°C and 70°C. The extraction results from each corn silk were analyzed for chemical and phytochemical characteristics.The optimum condition for extracting corn silk powder was obtained at 40oC and the extraction time was 60 minutes. In this condition, chemical content produced is as follow: 0.80% of soluble fiber, 0.58% of insoluble fiber, 1.38% of total fiber, 2.36% of moisture content, 2.50% of ash content, 1.29% of fat content, protein content of 16.93%, and carbohydrate content of 78.20%, and phytochemical content were total phenol of 5342.45µg EAG / g, total flavonoids of 167.45µg EAG / g, beta-sitosterol of 996.53 ppm, antioxidant activity of 50.41 %.

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<p><em>Kaempferia rotunda </em>that long ago been used to treat gastrointestinal disorders and also treat cancer, has<em> </em>promising opportunities to cultivate. There are several obstacles in cultivation today. Plant propagation by <em>in</em> <em>vitro </em>can be one solution.<em> </em>Basic media and plant growth promoting substance usage is an important factor which<em> </em>has to be concerned. Research aiming to produce high quality seed and in large numbers. Research has been carried out in Tissue Culture Laboratory, Agriculture Faculty, Sebelas Maret University, Surakarta, during July 2014 to August 2015. This research was used MS (Murashige and Skoog) media with combination treatment of each IBA and BAP concentration of 0, 1, 2, 3, and 4 ppm. The number of shoots that appear on the explant is the main observation variables. The results showed IBA and BAP are likely to affect the appearance of buds as much as 2 pieces in each explant at 0 ppm IBA treatment.</p>

Gamma-ray irradiation on Cempo Ireng can lead to the variation of morphological characteristics; hence, morphological characterization is necessary to determine changes in potential lines. This research aimed to characterize the potential lines as one of the requirements for proposing plant variety release and add black rice genetic diversity information. The research was carried out in the rice fields of Pakahan Village, Jogonalan, Klaten in January-June 2020 with the arrangement of potential lines 8,13 and 44 of M6 in a row. In total 30 plants were selected randomly for each potential line and observed 19 morphological characters. Data analysis was carried out descriptively and qualitatively with the Chi-Square test. The results showed that gamma-ray irradiation affects the characters of leaf angle, auricle color, ligule color, leaf-blade color, leaf surface, panicle type, grain color, apiculus color, and rice length. Potential line 8 had different characteristics compared to non-irradiated Cempo Ireng in the characters of auricle color, leaf-blade color, leaf width, panicle length, grain width, rice length, and rice width, while on potential lines 13 and 44 occur in the characters of the leaf surface and panicle length.