Cempo Ireng rice is local rice that is rich in anthocyanins. The issue with Cempo Ireng rice is its lengthy harvest life, high habitus, and low production. Plant mutations are one method for overcoming this. The study’s goal is to create better mutant plants with high yields. The study was conducted by placing three lines in plots from December 2019 to August 2020. The results were descriptively evaluated and compared to the control using the T-test. The results showed that the mutant plant was superior to black rice without irradiation (control). The line with the highest productivity value (per hectare) was line 51 which was 8.45 tons/ha. Line 44 has the highest average anthocyanin content of 75.10 ppm.

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Sapodilla (Achras zapota) is one type of tropical fruits with high economic value that are easily found in Indonesia. However, the number of productive plants has been decreased and affected the rate of fruit production. The objective of this research is to develop in vitro propagation of Achras zapota through meristem induction to provide great quality seedling. Type of explants (apical and lateral meristem) are cultured on Woody Plant Medium (WPM) and combined with different concentrations of BAP (0 ppm, 2 ppm, 3 ppm, 4 ppm, 5 ppm). Variables observed are shoot, leaf, root (emergence, length, number) and callus formation. The results show that all treatments able to induce shoot and leaf, but only several treatments able to induce root and callus formation. Lateral meristem is an appropriate explant to induce high number of leaves significantly and lateral meristem has the highest rate of multiplication 1.7 with application of BAP 2 ppm. The results can be used as reference to promote shoot multiplication. But further research is needed to determine proper combination to induce shoot multiplication significantly.

Climate change causes an increase in rice plant pests, one of them is brown planthopper. The effort through an assembly of black rice potential lines was made by mutation induction of Cempo Ireng black rice, but it’s only able to shorten the age of plant without being able to improve rice resistance to brown planthopper. Jeliteng as a new high yielding variety of black rice that is resistant to brown planthopper biotype 1 makes it worthy of being a potential parent source. Reciprocal crosses needed to be carried out to get offspring that have superior traits from both parents. This research aims to get F1 generation from the potential lines of black rice crossing with the Jeliteng variety and their reciprocals to improve local varieties of black rice. The research was conducted in the experimental field of the village of Gawanan, Sukoharjo Regency from August 2020 to April 2021. The planting materials were the Jeliteng variety as male parents while 6 potential lines of black rice 8, 13, 44, 46, 51, 52, and Cempo Ireng variety as female parents. Crosses were conducted by hand crossing technique on 14 sets. The data were analyzed descriptively and using the Chi-square test. The results showed that the crossing set could be crossed and produced fertile F1 generation. The length of grain, the width of grain, and the weight of a grain of the F1 varied in all sets. The skin colour of the F1 generation in all sets was mostly straw yellow with brown spots.

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