Virgin coconut oil is obtained by wet processing of coconut milk using fermentation, centrifugation, enzymatic extraction, and the microwave heating method. Presently, VCO has several positive effects and benefits to human health, hence, it is regularly consumed and widely known as a unique functional food. VCO contains lauric acid (45 to 52 %). By lipase in the digestive system, VCO can undergo a breakdown into lauric acid, 1-monolaurin, and 2-monolaurin. These components have both hydrophilic and lipophilic groups and are also recognized as excellent antimicrobial lipids. Furthermore, lauric acid and monolaurin can be used as antibacterial, antifungal, and antiviral with broad-spectrum inhibition. Lauric acid and monolaurin have a strong ability to destroy gram-positive bacteria, especially S. aureus, fungi such as C. Albicans, and viruses including vesicular stomatitis virus (VSV), herpes simplex virus (HSV), and visna virus (VV). Lauric acid and monolaurin interact with certain functional groups located in the cell membrane and can cause damage to the cell. In general, the potential of VCO as healthy food is contributed by lauric acid and monolaurin which are antimicrobial agents.

Desa Nggorang, Kecamatan Komodo, Maggarai Barat merupakan daerah yang cukup ideal untuk pengembangan ternak dan pemanfaatan limbah pertanian karena didukung oleh beberapa faktor antara lain daya dukung wilayah tempat sumber air yang ada dapat memenuhi air masyarakat kebutuhan sepanjang tahun dan ketersediaan limbah pertanian jerami padi. Tujuan dari pelaksanaan pelatihan pemrosesan pakan amoniase adalah untuk memaksimalkan produktivitas peternakan sapi dengan memanfaatkan ketersediaan sumber daya alam sambil memberdayakan limbah pertanian jerami padi sebagai sumber pakan utama dengan memanfaatkan teknologi pemrosesan pakan. Oleh karena itu, pelatihan teknologi pengolahan pakan jerami telah menjadi limbah pertanian terbesar di Desa Nggorang. Berdasarkan hasil kegiatan yang ada, ada peningkatan pemahaman petani yang dicapai. Dengan demikian desa Nggorang diharapkan mampu meningkatkan kemandirian masyarakat desa melalui kelompok tani mitra yang mampu mengoptimalkan produktivitas ternak melalui kemandirian dan kecukupan pakan ternak di Desa Nggorang, Kecamatan Komodo, Manggarai Barat, NTT  Kata Kunci: Agrowisata, Amoniase, Nggorang, Komodo

The purpose of this study was to isolate and identify the lactic acid bacteria found in Sumba mares milk. This research will be carried out from September to November 2017 at the Veterinary Laboratory of the Livestock Service Office of East Nusa Tenggara Province. The research stages are isolation of lactic acid bacteria from Sumba mares milk by growing lactic acid bacteria on MRS agar media, and identifying lactic acid bacteria with gram staining, catalase test, motility test, and total plate count testing. Based on the results of the study, the presence of lactic acid bacteria originating from Sumba mares milk was characterized by the presence of colonies of lactic acid bacteria from Sumba mares milk which grew on MRS media agar as a selective medium for lactic acid bacteria. Based on the results of testing the characteristics of lactic acid bacteria Sumba mares milk has Gram positive, the negative results in the catalase test are characterized by not forming gas bubbles when the bacteria are added with H2O2. Based on the motility test obtained negative results or bacteria are non-motile and the number of lactic acid bacteria that can be a total picture of lactic acid bacteria that exist in Sumba mares milk in milliliter of milk. The conclusion are lactic acid bacteria isolated from Sumba mares milk has characteristic as Gram positive, basil or stem shaped, negative catalase and non motile, and has a total lactic acid bacteria of 3.5 x 108 cfu/ml (est).    

Zoonotic desease transmission can happen in every stage of animal-orign food production, from farm to table. This study is counducted to evaluate the understanding of farmers and animal-origin food product enterprises about zoonotic diseases and their prevention in Kupang City. The study was conducted 6 region with 200 respondents from farmers, 30 respondents from buthcers, 30 respondents from meat distributors, 30 respndents from meat seller and 30 respondents from food industry. The result than analyzed by descriptive analysis and than presented in graphics and tables. The result shows that the understanding of farmers and animal origin food product enterprise in Kupang City about zoonotic diseases is low so that their behavior, in attempt to prevent zoonoses, is not maximal. In farmers, 87,5% of farmers do not have a proper check up for their animal’s health and 30,5% of them still consume meat from sick and dead animals. In butchers, 80% of butchers do not have a hand-washing facility, 86,7% do not have a seperated room for meat and visceral handling, 96,7% do not use a proper foot-wear when working, and 43,3% of them stil use water from the river or from gutters. In meat distributors, 90% of them only use bike as vehicle for the distribution. In meat sellers, 96,7% of meat sellers allow their customer to directly touch the product, 80% do not have a hand-whashing facility and 100% of them do not have a refrigerator as a storaging vacility. In food industry, 90% of them have a good cleaning behavior, wash their hand with flowing water and soap, and have a refrigerator as storaging vacility. It can be conlcuded that food industry is the only stage with good behavior in atempt to prevent zoonotic desease transmission.

This study aims to evaluate the ability of lactic acid bacteria isolated from Sumba horse milk as a starter in improving the quality of rice straw silage. Determination of the quality of rice straw silage through organoleptic tests, measurement of percentage of silage damage and silage pH. there are 7 treatments based on silage compositions and lactic acid bacteria. The results showed the presence of golden color in all treatments as the original color, the texture was very soft to soft and not slimy, and the presence of a distinctive silage odor or close to the typical smell of silage in all treatments. The presentation of silage damage to rice straw in the positive control group showed the highest percentage of damage that was equal to 43.58%, while the percentage of damage in the treatment group ranged from 28.57% to 41.02%. The pH results showed the control group had a pH of 3.6 and the P1-P6 group had an average pH ranging from 5.7 to 6.5. The addition of lactic acid bacteria in the manufacture of silage in rice straw provides good quality silage results.

Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prevalence of Anisakis sp. in Epinephelus sp. was 22% (n=11/50). The parasite was mostly found in abdominal cavity, gonad, intestines, and muscles of fish. Conclusion: This study suggests that Anisakis sp. is present in Epinephelus sp., exerting potential health hazards for human who consume the fish.

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