Zoonotic desease transmission can happen in every stage of animal-orign food production, from farm to table. This study is counducted to evaluate the understanding of farmers and animal-origin food product enterprises about zoonotic diseases and their prevention in Kupang City. The study was conducted 6 region with 200 respondents from farmers, 30 respondents from buthcers, 30 respondents from meat distributors, 30 respndents from meat seller and 30 respondents from food industry. The result than analyzed by descriptive analysis and than presented in graphics and tables. The result shows that the understanding of farmers and animal origin food product enterprise in Kupang City about zoonotic diseases is low so that their behavior, in attempt to prevent zoonoses, is not maximal. In farmers, 87,5% of farmers do not have a proper check up for their animal’s health and 30,5% of them still consume meat from sick and dead animals. In butchers, 80% of butchers do not have a hand-washing facility, 86,7% do not have a seperated room for meat and visceral handling, 96,7% do not use a proper foot-wear when working, and 43,3% of them stil use water from the river or from gutters. In meat distributors, 90% of them only use bike as vehicle for the distribution. In meat sellers, 96,7% of meat sellers allow their customer to directly touch the product, 80% do not have a hand-whashing facility and 100% of them do not have a refrigerator as a storaging vacility. In food industry, 90% of them have a good cleaning behavior, wash their hand with flowing water and soap, and have a refrigerator as storaging vacility. It can be conlcuded that food industry is the only stage with good behavior in atempt to prevent zoonotic desease transmission.
Beef is one type of meat that is quite popular with almost all Indonesian people, especially in East Nusa Tenggara (NTT). Storage of beef at room temperature and open space can accelerate the occurrence of decay in meat. This is because the complete nutritional content and high water content in meat can be a good medium for the growth of pathogenic bacteria or spoilage bacteria. One of the efforts that can be done to prevent meat rot is to do a natural preservative method by utilizing plant parts that contain antimicrobial compounds. Moringa leaves are one part of the plant which is known to have antimicrobial compounds. This study aims to determine the effect of Moringa oleifera Lamk leaf extract on the microbiological and organoleptic quality of beef. This research is an experimental laboratory research. A total of 48 thigh beef (Biceps femoris) beef samples were tested in this study using a completely randomized factorial pattern design, namely concentration factors of 0% (K0), 5% (K1), 10% (K2), and 15% (K3) extract Moringa leaf and long storage factor at room temperature are 0 hours, 6 hours, 12 hours, and 18 hours and repeated three times. The parameters tested were color quality, texture, aroma, initial decay test, pH test, and total plate count (TPC) test. The results showed changes in the color, aroma, and texture of the meat. The Eber test shows the K3 group can last up to 18 hours. There was a very significant difference between the concentration of Moringa leaf extract on the length of storage of meat and the pH value of meat (P<0,01). Moringa leaf extract concentration factors and meat storage duration significantly influence the TPC value (P<0,01). The TPC value in the K3 group is below the SNI contamination limit for storage room temperature less than 18 hours.
Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prevalence of Anisakis sp. in Epinephelus sp. was 22% (n=11/50). The parasite was mostly found in abdominal cavity, gonad, intestines, and muscles of fish. Conclusion: This study suggests that Anisakis sp. is present in Epinephelus sp., exerting potential health hazards for human who consume the fish.
Misuse of antibiotics has the potential to accelerate the emergence of types that are resistant to inappropriate drugs. This study aims to evaluate the inappropriate use of antibiotics in pig farms in the City of Kupang, East Nusa Tenggara. This antibiotic use evaluation study was conducted from March to May 2018. This study was conducted on 100 pig farmers spread across 6 (six) districts in Kupang City. The sampling technique was done by stratified random sampling method based on the characteristics or types of the population. This research used descriptive analysis method from the results of questionnaire data collection that had been filled out by pig farmers in Kupang City. Based on the results obtained that 84% of pig farmers or respondents did not know the function of antibiotics, and 83% of respondents did not know that the administration of antibiotics must be done with the supervision of a veterinarian. The results also showed that 86% of pig farmers buy antibiotics without a veterinarian’s prescription, with the type of antibiotics most often used were the tetracycline and sulfonamide groups. These results conclude that dominant respondents apply antibiotics without veterinary supervision, which can contribute to the spread of resistant bacteria so that it adversely affects human health.
Pork is one of the most consumption types of meat in the East Nusa Tenggara region.. Moringa leaf are one part of the plant which is known to have antimicrobial compounds.The aimof this research was to determine the influence of Moringa leaf infusion as a natural preservative to the quality of pork.This research is an experimental laboratory research and used a total of 48 samples of thigh pork (Biceps femoris). This research used a completely randomized design with factorial pattern. The first factor was concentration of infusion Moringa leaf consist of 0% (K0), 5% (K1), 10% (K2), and 15% (K3) and the second factor was time of storage consist of 0,6, 12, and 18 hours with 3 replications. The parameters of porkquality that have been examined: color, texture, odor, value in the Eber test, pH value, and total plate count (TPC) value.The results showed that the addition of Moringa leaf infusion change the color and odor. The Eber test shows the K3 group can last up to 18 hours. There was no significant effect of infusion concentration (P> 0.05) on the pH value and there was a very significant effect on the time of storage (P<0.01) on the pH value. There was a significant effect of infusion concentration (P<0.05) and very significant effect on the time of storage (P <0.01) on the TPC value. The value of TPC in the K3 group was below of the Standar Nasional Indonesia contamination limit for laying less than 12 hours at room temperature.
The aims of this study were to know the influence of education level and type of work against the level of understanding and application hygiene and sanitation processing of chicken meat by housewives in Kupang City. Determination the quantity of samples is done with software Win Episcope® 2.0 with level of confidence 95%, expected prevalence 50%, and accepted error 5%, then total of 220 respondents involved in this study. Samples carried out by simple random sampling to determine the area (RW) that elected per sub district. Assessment the level of understanding and application of sanitary hygiene is performed by summing the score answers of each item contained in the questionnaire. The data were analyzed descriptively and correlation between the characteristics of the respondents determined by Spearman test. The results showed that most housewife with low education level and not working have a good level of understanding (n=81; 73.6%) and application of sanitation hygiene categorized enough (n=65; 59.1%), housewives with low levels of education and work have a good level of understanding (n=77; 70%) and the application of sanitation hygiene categorized enough (n=65; 59.1%). Spearman correlations test show that there were noticeable at low-educated housewife and not working (p<0.05), and low-educated housewife and working (p<0.05).
Pengendalian nyamuk yang berperan sebagai vektor merupakan tindakan terpenting dalam penanggulangan penyakit seperti filariasis.Filariasis merupakan penyakit menular yang disebabkan oleh cacing filaria yang menyerang saluran dan kelenjar getah bening yang ditularkan oleh berbagai jenis nyamuk. Kasus filariasis di Kabupaten Sikka tahun 2015- 2017 setiap tahunnya meningkat. Penggunaan insektisida menjadi pilihan utama masyarakat dalam pengendalian vektor nyamuk. Insektisida yang digunakan secara terus-menerus dapat menyebabkan nyamuk menjadi resisten. Penelitian ini bertujuan untuk mengetahui status resistensi vektor filariasis terhadap insektisida permethrin 0,75% di Kabupaten Sikka tahun 2018. Penelitian ini diawali dengan survei lokasi dan pengambilan sampel di Kabupaten Sikka dan dilanjutkan dengan pemeliharaan nyamuk serta uji resistensi. Bahan yang digunakan dalam penelitian ini adalah nyamuk Aedes sp. dan Anopheles sp., impregnated paper permethrin 0,75%. Hasil uji resistensi menggunakan metode susceptibility test dengan impregnated paper permethrin 0,75% adalah kematian nyamuk uji terhadap permethrin adalah 21,3% sehingga disimpulkan bahwa vektor filariasis di Kabupaten Sikka dinyatakan telah resisten tinggi terhadap insektisida permethrin 0,75% karena kematian nyamuk uji <90%.
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