(Bt) is a ubiquitous pathogenic bacterium exploited worldwide in successful insect pest management programs. The present study aimed at resolving the diversity status and genetic structure of Bt isolates collected from unique and native ecological niche, north-western Indian Himalayas.Forty five ( ) isolates native to Uttarakhand, India were analyzed for their genetic diversity using Arbitrary Primed-Polymerase Chain Reaction (AP-PCR) patterns (ERIC1, ERIC2, BOX and GTG) and gyrase ( ) sequences.Amongst the AP-PCR patterns, ERIC and BOX primers clearly indicated the genetic inconsistency of by differentiating low and high hill isolates with unique banding pattern. A dendrogram generated showed fastidious clustering with coexistence of attitudinally related strains, indicating the genetic relatedness with respect to geographical proximity. The (285 bp) dependent phylogeny of seven selected isolates revealed their genetic proximity with different serovars.The present study demonstrates that ERIC and BOX-PCR can be used in establishment of geographical variation and in phylogenetic relatedness with serovars. Genetic relatedness between isolates was found to be a function of geographical proximity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.