Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds

Clemente, Aline da Consolação Sampaio; Carvalho, María Laene Moreira de; Guimarães, Renato Mendes

doi:10.1590/s1413-70542012000400005

Cited by 11 publications

(22 citation statements)

References 11 publications

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“…The results were expressed in percentage of viable embryos. The methodology was adapted because of the size of the sample for the test, using half the amount of grains necessary in the test according to the methodology of Clemente et al (2012).…”

Section: Methodsmentioning

confidence: 99%

Simultaneous optimization of coffee quality variables during storage

Abreu

Rosa

Cirillo

et al. 2017

Rev. bras. eng. agríc. ambient.

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A B S T R A C TThe objective of the study was to use methodology of simultaneous optimization of multiple responses applied to an experimental design to determine the best combination of storage period and conditions for preservation of coffee beans. Coffea arabica L. fruits were harvested in the ripe stage of maturation, processed using wet and dry methods, and dried to 11% (wet basis) moisture content. Part of the beans was hulled, while the other part was hulled only after the beans were stored under two different environmental conditions: cooled air at 10 ºC with 50% relative humidity; and at 25 ºC without controlling the relative humidity. Samples were taken at 3, 6, and 12 months intervals in order to evaluate quality. The data were submitted to the simultaneous optimization of responses for each processing and hulling condition separately, in a completely randomized design and 2 x 3 factorial scheme (two storage conditions and three storage periods). In conclusion, the use of the simultaneous optimization of responses is viable to be applied for determining the ideal storage conditions in a refrigerated condition. Otimização simultânea de variáveis da qualidade do café durante armazenamento R E S U M OObjetivou-se utilizar a metodologia de resposta simultânea aplicada a um delineamento experimental para determinar a melhor combinação de tempo e das condições de armazenamento para a conservação de grãos de café. Frutos de Coffea arabica L. foram colhidos no estádio de maturação cereja, processados por via úmida ou por via seca e secados até atingir 11% de teor de água. Parte dos grãos foi beneficiada e parte não foi beneficiada antes de ser armazenada em duas condições de ambiente: em ar refrigerado a 10 ºC e umidade relativa de 50% e em 25 ºC sem controle da umidade relativa do ar. Nos períodos de 3, 6 e 12 meses, foram retiradas amostras para avaliação da qualidade. Os dados foram submetidos à técnica de otimização de resposta simultânea para cada processamento e beneficiamento separadamente, em delineamento inteiramente casualizado e esquema fatorial 2 x 3 (duas condições de armazenamento e três períodos de armazenamento). Conclui-se que a utilização da metodologia de resposta simultânea é viável para ser aplicada para determinação das condições ideais de armazenamento em condição refrigerada.

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Section: Methodsmentioning

confidence: 99%

Simultaneous optimization of coffee quality variables during storage

Abreu

Rosa

Cirillo

et al. 2017

Rev. bras. eng. agríc. ambient.

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“…Pengembangan metode pengujian mutu benih kopi sangat diperlukan untuk mempersingkat waktu pengujian. Beberapa kajian telah dilakukan untuk mengurangi lamanya waktu pengujian, diantaranya adalah evaluasi secara visual potensi perkecambahan (Sera & Miglioranza 2003), uji lercafe (Zonta et al 2009), evaluasi morfologi fase perkecambahan benih (Da Rosa et al 2010), dan uji tetrazolium (Clemente et al 2012).…”

Section: Pendahuluanunclassified

PERBANDINGAN UJI TETRAZOLIUM DAN RADICLE EMERGENCE DALAM MENDUGA VIABILITAS BENIH KOPI ARABIKA (Coffea arabica L.) / The Comparison of Tetrazolium and Radicle Emergence Test to Estimate the Viability of Arabica Coffee (Coffea arabica L.) Seeds

Pramana¹,

Pujiasmanto²,

Sakya³

2019

j. penelitian tanam. industri

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Development of seed testing methods needs to be conducted in order to obtain fast, precise and applicable result in the field. The objective of this study was to compare the two rapid test methods of Arabica coffee seed viability, the tetrazolium and radicle emergence tests. The experiment was conducted at Quality Seed Testing Laboratory, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Surabaya from beginning of January until March 2018. Three lots of Arabica coffee (Coffea arabica var. Sigarar Utang) (Lot A = harvest in 1st week of August, 2017; Lot B = 3rd week of August, 2017; and Lot C = 1st week of September, 2017) were obtained from Indonesian Coffee and Cocoa Research Institute, Jember. The experiments used completely randomized design with four replications. The result showed that the viability test using TZ was faster (4 days) than using RE (14 days). Regression equation models for estimating seed viability are less precise, while the estimation method of paired sample t-test was sufficient to predict the actual value of the seed viability. Redicle emergence is longer than tetrazolium which is for 14 days. The regression equation model of the radicle emergence is sufficient to predict the seed physiological quality parameter.Keywords: viability, tetrazolium, radicle emergence, coffee seed AbstrakPengembangan metodologi uji benih untuk memperoleh hasil yang cepat, tepat dan aplikatif di lapangan. Tujuan penelitian adalah membandingkan dua metode pengujian viabilitias benih kopi Arabika, yaitu uji tetrazolium dan radicle emergence (kemunculan radikula). Penelitian dilaksanakan di Laboratorium Pengujian Mutu Benih, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Surabaya, mulai bulan Januari sampai Maret 2018. Tiga lot benih kopi Arabika varietas Sigarar Utang (Lot A : panen minggu I bulan Agustus 2017, Lot B : minggu III bulan Agustus 2017, Lot C : minggu I bulan September 2017) diperoleh dari Pusat Penelitian Kopi dan Kakao Indonesia. Rancangan yang digunakan adalah rancangan acak lengkap satu faktor (lot benih) dengan empat kali ulangan. Hasil penelitian menunjukkan bahwa pengujian viabilitas kopi dengan metode TZ lebih cepat (4 hari) dibandingkan dengan metode kemunculan radikula (14 hari). Penggunaan model regresi untuk menduga viabilitas biji kurang tepat, sementara penggunaan t-test berpasangan sangat tepat untuk menduga nilai yang sebenarnya dari viabilitas biji. Oleh karena itu, metode pendugaan mutu benih kopi Arabika berdasarkan kemunculan radikula lebih tepat untuk digunakan di lapangan.Kata kunci: viabilitas, tetrazolium, kemunculan radikula, benih kopi

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“…The tetrazolium test is performed in a shorter time, thus reduces possible adverse factors, for example, attack of microorganisms, in which case can occur in the germination test affecting the test evaluation, and it does not happen in the tetrazolium test. Thus, small differences can be found between the results of both tests (Clemente et al, 2012;Krzyzanowski et al, 1999). On the other hand, differences between results of the germination test and the tetrazolium test have been observed when evaluating the physiological quality of coffee seeds Coelho, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…The tetrazolium test was conducted with four replicates of 25 coffee seeds, which were soaked in water for 36 h for extraction of embryos, and kept in 0.5% tetrazolium solution at 30°C for 3 h. The embryos were ranked into viable and non-viable according to location and extent of damage (Clemente et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Relevance of endo--mannanase enzyme in coffee seed deterioration process

Freitas¹,

Rosa²,

Clemente³

et al. 2017

Afr. J. Agric. Res.

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The endo-β-mannanase acts on the hemicellulose fraction of the endosperm cell walls, mainly mannans and galactomannans. This process weakens cell walls and allows radicle protrusion during seed germination, but may also occur during the deterioration process. Thus, the aim of this research was to determine the activity of endo-β-mannanase enzyme in dry coffee seeds and in soaked seeds, evaluating its relationship between physiological qualities. Coffee seeds obtained by different processing methods (natural, fermented and demucilated) and drying (sun, shade and dryer) were used. Seed quality was evaluated by germination and tetrazolium tests, and the endo-β-mannanase enzyme activity was determined in dry seeds and after 10 days of soaking. From the results, it was concluded that there is significant inverse relationship between the physiological quality of coffee seeds and the expression of endo-β-mannanase, and seeds with lower percentages of germination and viability of embryos have a higher activity of the enzyme. After ten days of soaking, coffee seeds had higher expression of endo-β-mannanase as compared to the dry seeds for all treatments of fruit processing and drying.

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Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds

Cited by 11 publications

References 11 publications

Simultaneous optimization of coffee quality variables during storage

Simultaneous optimization of coffee quality variables during storage

PERBANDINGAN UJI TETRAZOLIUM DAN RADICLE EMERGENCE DALAM MENDUGA VIABILITAS BENIH KOPI ARABIKA (Coffea arabica L.) / The Comparison of Tetrazolium and Radicle Emergence Test to Estimate the Viability of Arabica Coffee (Coffea arabica L.) Seeds

Relevance of endo--mannanase enzyme in coffee seed deterioration process

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