Purification and biochemical characterization of novel acidic chitinase from Paenicibacillus barengoltzii

Fu, Xing; Yan, Qiaojuan; Wang, Jing; Yang, Shaoqing; Jiang, Zhengqiang

doi:10.1016/j.ijbiomac.2016.06.050

Cited by 55 publications

(36 citation statements)

References 39 publications

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“…Similar to most of the other chitinolytic enzymes-producing strains of Paenibacillus species, only one chitinase or chitosanase were purified from the culture supernatant [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. However, the molecular mass of TKU029 chitosanase (63 kDa) estimated by SDS-PAGE ( Figure 3 ) differed from the chitinases or chitosanases of the other Paenibacillus strains, for instance, P. pasadenensis NCIM5434 chitinase (35 kDa) [ 23 ], Paenibacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans

et al. 2018

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Chitinous materials from marine byproducts elicit great interest among biotechnologists for their potential biomedical or agricultural applications. In this study, four kinds of marine chitinous materials (squid pens, shrimp heads, demineralized shrimp shells, and demineralized crab shells) were used to screen the best source for producing chitosanase by Paenibacillus macerans TKU029. Among them, the chitosanase activity was found to be highest in the culture using the medium containing squid pens as the sole carbon/nitrogen (C/N) source. A chitosanase which showed molecular weights at 63 kDa was isolated from P. macerans cultured on a squid pens medium. The purified TKU029 chitosanase exhibited optimum activity at 60 °C and pH 7, and was stable at temperatures under 50 °C and pH 3-8. An analysis by MALDI-TOF MS revealed that the chitosan oligosaccharides (COS) obtained from the hydrolysis of water-soluble chitosan by TKU029 crude enzyme showed various degrees of polymerization (DP), varying from 3–6. The obtained COS enhanced the growth of four lactic acid bacteria strains but exhibited no effect on the growth of E. coli. By specialized growth enhancing effects, the COS produced from hydrolyzing water soluble chitosan with TKU029 chitinolytic enzymes could have potential for use in medicine or nutraceuticals.

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Section: Resultsmentioning

confidence: 99%

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans

et al. 2018

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“…7). Several workers have reported broad range of pH optima for chitinase, from 4.0 to 10.0 for Paenibacillus pasadenensis CS0611 (Xiaoxiao et al, 2017), 4.0 to 10.0 for Paenibacillus thermoaerophilus TC22-2b (Ueda & Kurosawa, 2015), from 3.0 to 9.0 for Paenibacillus barengoltzii (Fu et al, 2016).…”

Section: Effect Of Different Ph Values On Chitinase Productionmentioning

confidence: 99%

“…(Singh, 2010), Paenibacillus sp. BISR-047 (Savita et al, 2013), Paenibacillus barengoltzii (Fu et al, 2016). most of the industrial processes which depend on enzymatic reactions are performed at high temperatures, thus in the last few years, researches focusing on thermostable enzymes has been increased.…”

Section: Effect Of Temperature and Time On Stability Of Chitinasementioning

confidence: 99%

Efficacy of Thermophilic Soil-Isolated Paenibacillus sp. NBR10 as a Chitinolytic and Biocontrol Bacterium-In vitro Study

et al. 2018

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A mONG microbial enzymes, chitinases received increased attentions due to their wide applications especially in agriculture sector for biocontrol of fungal phytopathogens. The growing interest in chitinases with thermostability nature, forced many researchers to isolate and characterize novel chitinase producing-bacteriafrom extreme environments. The present study describe characterization of thermostable chitinase produced by thermophilic soilisolated bacterial strain NBR10 from Rafha governorate, Saudi Arabia. Chitinase producing NBR10 strain was identified as Paenibacillus sp. according to the traditional methods of morphological, biochemical and physiological characteristics coupling with sequencing of 16S rRNA (GenBank accession number KT957624.1). The isolated strain was found to have a potent antifungal against three of the phytopathogenic fungi Fusarium oxysporum, Alternaria burnsii and Rhizoctonia solani with percent of growth inhibition 52.5 75.0 and 85.71%, respectively. Chitinase obtained from Paenibacillus sp. NBR10 showed activity at ranges of temperature (30-90°C) and pH (4-9), showing optimum activity at 55°C and pH 7.0, respectively. Also, it exhibited high thermostability at higher temperatures, where 90% of its activity is retained at 65°C for 36h. The promising chitinolytic and antifungal activity of locally isolated Paenibacillus sp. NBR10, candidate this strain to be used as a potential biological control agent.

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“…Studies also reported specific featured chitinases with respect to pH (Loni et al, 2014;Fu et al, 2016) and temperature ( Karthik et al, 2015) having potential in industrial applications. Besides, identification of optimum pH and temperature of any given bacterial chitinase is important to predict its activity in different environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

Characterization of antifungal Paenibacillus illinoisensis strain UKCH21 and its chitinolytic properties

Subbanna¹,

Khan²,

Shivashankara³

2016

Afr. J. Microbiol. Res.

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Paenibacillus strain, UKCH21, isolated from Uttarakhand State, north western Indian Himalayas was found to produce high levels of extracellular chitinases. The 16s rRNA gene sequence showed 100% homology with P. illinoisensis (Accession No. KR856190) available in the public domain and further phylogenetic study also verified the species identity. The culture supernatants have a maximum chitinase activity of 110.8 U/ml after 3 days of culturing. The isolate showed strong antifungal activity manifested in the form of progressive mycelia degradation in dual culture plates. The pathogenicity was observed as structural deformities like uneven thickening of mycelia as a result of direct degradation of chitin. The optimum pH and temperature of UKCH21 chitinases was found to be 5 and 50°C, respectively. Partial characterization of chitinase gene also confirms the family 18 status of glycosyl hydrolase with substantial variability presented here with. Above all, percent inhibition of growth and the rapid degradation of mycelia of tested plant pathogenic fungi (Rhizoctonia solani, Fusarium solani and Sclerotium rolfsi) in bacteria seeded medium suggest its utilization as potent antifungal biocontrol agent.

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Purification and biochemical characterization of novel acidic chitinase from Paenicibacillus barengoltzii

Cited by 55 publications

References 39 publications

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans

Efficacy of Thermophilic Soil-Isolated Paenibacillus sp. NBR10 as a Chitinolytic and Biocontrol Bacterium-In vitro Study

Characterization of antifungal Paenibacillus illinoisensis strain UKCH21 and its chitinolytic properties

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