Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: methanol: dichloromethane: ammonia (10:6:3:1 v/v), ethyl acetate: methanol: ammonia (15:4:1 v/v), and methanol: acetonitrile: ammonia (9:4:1 v/v) for almotriptan, eletriptan and rizatriptan respectively. The TLC plates were scanned at 235 nm. Linear relationships were obtained over concentration ranges (5–50 μg/spot) for almotriptan and rizatriptan, and (5–60 μg/spot) for eletriptan. The second method based on the separation and determination of the studied drugs, using RP-HPLC technique. The separation was achieved on C18 Hypersil column, elution was carried out using phosphate buffer pH 3: methanol: acetonitrile (2: 1:1 v/v) at flow rate 2 mL/min and UV detection at 235 nm. Linear relationships were obtained over concentration ranges (10–200 μg/mL) for almotriptan and eletriptan, and (10–180 μg/mL) for rizatriptan. The chromatographic methods were successfully applied for the determination of each of the studied drugs in pure form, tablet form, and in laboratory prepared mixtures with their acid degradation products.
For a long time, nanoparticle biosynthetic discipline is still under development and is known to have a big impact on numerous manufactures. Synthesis of nanoparticles by green methods with antimicrobial properties is of great researchers concern in the explored of new pharmaceutical and biomedical products. In this study, synthesis of silver nanoparticles (AgNPs) have been carried out using aqueous extract of lemon peel (Citrus limon), which acts as encapsulating cage for the silver nanoparticles. Synthesized AgNPs was monitored by formation of brown color, confirmed by UV/VIS spectra, which showed appearance of a surface plasmon resonance (SPR) band. FT-IR analysis of the bioextract after the addition of silver solution showed strong bands at 1021, 1443, 1634 and 3428 cm −1. Transmission electron microscopy examination of the solution containing AgNPs demonstrated spherical particles within nanoranged from 9.3 nm to 20.3 nm with the main diameter of 15.76 nm. The biologically synthesized AgNPs showed a promising antimicrobial activity, where the maximal growth inhibition was recorded for both Pseudomonas (P.) aeruginosa and Escherichia (E.) coli-21.5 ± 1.3 and 19.0 ± 0.20 mm, respectively. While the minimum growth inhibition was recorded for Gram positive bacteria Bacillus subtilis (B.) and Staphylococcus (S.) aureus-15.0 ± 0.20 and 16.5 ± 1.50 mm, respectively. At the same time weak antifungal activity was observed for both Aspergillus (A.) flavus and Candida (C.) albicans-14.0 ± 0.15 and 12.5 ± 0.45 mm, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.