Two chromatographic Methods for the Determination of some Antimigraine Drugs

Abstract

Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: methanol: dichloromethane: ammonia (10:6:3:1 v/v), ethyl acetate: methanol: ammonia (15:4:1 v/v), and methanol: acetonitrile: ammonia (9:4:1 v/v) for almotriptan, eletriptan and rizatriptan respectively. The TLC plates were scanned at 235 nm. Linear relationships were obtained over concentration ranges (5–50 μg/spot) for almotriptan and rizatriptan, and (5–60 μg/spot) for eletriptan. The second method based on the separation and determination of the studied drugs, using RP-HPLC technique. The separation was achieved on C18 Hypersil column, elution was carried out using phosphate buffer pH 3: methanol: acetonitrile (2: 1:1 v/v) at flow rate 2 mL/min and UV detection at 235 nm. Linear relationships were obtained over concentration ranges (10–200 μg/mL) for almotriptan and eletriptan, and (10–180 μg/mL) for rizatriptan. The chromatographic methods were successfully applied for the determination of each of the studied drugs in pure form, tablet form, and in laboratory prepared mixtures with their acid degradation products.

Chemical and pharmacological characterization of triptans

“…ciklohexán-diklórmetán-dietilamin valamint kloroform-metanol-etilacetát-ammónia. Egy másik közlemény butanol-ecetsav-víz mozgófázisokról számol be, különböző szerves módosítókkal [29][30].…”

A New extractive spectrophotometric method for determination of rizatriptan dosage forms using bromocresol green

“…HPLC [7-9] and HPTLC [10] methods have also been used for quantitative determination of rizatriptan in bulk drug or pharmaceutical dosage forms. Furtheremore, few spectrophotometric methods were reported in the literature for the determination of rizatriptan in dosage forms [11-16].…”

Utility of Charge Transfer and Ion‐Pair Complexation for Spectrophotometric Determination of Eletriptan Hydrobromide in Pure and Dosage Forms

“…Literature survey revealed that very few analytical methods have been reported for the determination of eletriptan in pure drug, pharmaceutical dosage forms, and biological samples using HPLC [4][5][6] and LC-MS [7] techniques. A few methods are found in the literature for the determination of ELT in pharmaceuticals and include spectrophotometry [8][9][10][11][12] and spectrofluorimetry [11,12].…”