Polygalacturonase production by Kluyveromyces marxianus: effect of medium composition

Abstract

A strain of Kluyveromyces marxianus (CCT 3172), isolated from a cocoa fermentation in Brazil, secreted an endopolygalacturonase (PG) when grown under self‐induced anaerobic conditions; neither polymethylesterase nor pectate lyase appeared in culture filtrates. Replacing glucose in the medium with sucrose had no effect on PG secretion or ethanol production. Growth in fructose‐containing medium retarded secretion of PG and ethanol, but had no effect on growth. Growth and ethanol production in media containing galactose resembled those in fructose‐containing medium, although PG secretion was lowered. Growth and PG secretion were considerably retarded in xylose‐containing medium, and were similarly affected in media containing different concentrations of glucose. Varying the concentration of ammonium sulphate in media had no effect on growth or PG secretion.

Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots

“…This effect has been reported in Saccharomyces cerevisiae (BLANCO et al, 1994), Cryptococcus albidus (FEDERICI, 1985), and Kluyveromyces marxianus (SCHWAN;ROSE, 1994). By contrast, W. anomalus does not exhibit this effect, and PG seems to show inducible synthesis.…”

“…Several authors reported that most of the pectinolytic yeasts are unable to use pectin or its derivatives such as CES (BLANCO et al, 1994;SCHWAN;COOPER;WHEALS, 1997). Fernández-González et al (2004) reported that several yeasts isolated from wine ecosystems were unable to grow when GA was the sole CES in YNB medium.…”

Metodologia para elaboração de fermentado de cajá (Spondias mombin L.)

“…Da cultura purificada, tomou-se uma alíquota para iniciar a formação do inóculo. Nesta fase, a levedura cresceu em 120mL, em frascos erlenmeyer, de meio MF, segundo SCHWAN & ROSE [40], constituído de (em g/L): glicose, 10,0; KH 2 PO 4 , 4,5; (NH 4 ) 2 SO 4 , 3,0; extrato de levedura 1,0;mgSO 4 ·7H 2 O, 0,250 e CaCl 2 , 0,250; pH 5,0. Os frascos com a levedura foram incubados em estufa, provida de agitador orbital, a 28°C e 30rpm.…”

Influence of carbon source, pH, and temperature on the polygalacturonase activity of Kluyveromyces marxianus CCMB 322

“…In addition, a study with a strain of Kluyveromyces marxianus CCT 3172, published by Schwan and Rose (1994), reported that no significant increase in growth or enzyme secretion was found when pectin, pectic acid, or polygalacturonic acid was added to media containing 1% (w/v) glucose.…”

“…Enzymes which hydrolyze pectic substances are broadly known as pectinases that include polygalacturonase, pectin esterase, pectin lyase, and pectate lyase on the basis of their mode of action (SCHWAN;ROSE, 1994).…”