Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots

Martos, María Alicia; Zubreski, Emilce R.; Combina, Mariana; Garro, Oscar Alfredo; Hours, Roque Alberto

doi:10.1590/s0101-20612013005000047

Cited by 20 publications

(18 citation statements)

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“…La biomasa total producida fue de 4,013 ± 0,26 gx/l, obteniéndose un rendimiento (Yx/s) de 0,401 gx/gs (yx/s = 0,466 C-molx/C-mols) en base a glucosa. De la curva de crecimiento microbiano se deter- ) (Martos et al, 2013a).…”

Section: Resultados Y Discusiónunclassified

“…Wickerhamomyces anomalus, aislada a partir de frutas cítricas en la provincia de Misiones, Argentina (Martos et al, 2013a).…”

Section: Microorganismounclassified

“…Esta enzima es de gran importancia industrial ya que posee actividad protopectinasas (PPasa) o capacidad macerante de tejidos vegetales (papa, mandioca, zanahoria, etc.) (Martos et al, 2013a, Martos et al, 2013b. La potencial aplicación industrial de esta enzima justifica la realización de estudios relacionados con su producción a mayor escala.…”

Section: Introductionunclassified

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Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la producción de una poligalacturonasa

Martos

Butiuk

Rojas

et al. 2014

Rev. Colomb. Biotecnol.

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ResumenWickerhamomyces anomalus, una levadura aislada de frutas cítricas en la provincia de Misiones, Argentina, produce una poligalacturonasa (endo-PG) con capacidad macerante de tejidos vegetales. El objetivo del presente trabajo fue determinar los parámetros cinéticos y estequiométricos del crecimiento de W. anomalus y la producción de la enzima poligalacturonasa en un medio de cultivo sintético, operado en sistema por lote, en un biorreactor a escala laboratorio. Los cultivos se realizaron en un biorreactor de 4 l que contenía 3 l de un medio sintético compuesto por glucosa, pectina de citrus, vitaminas, aminoácidos, sulfato de amonio y sales, y se incubaron con agitación y aireación, a 30 °C durante 12 h. El transcurso del proceso fermentativo se siguió por medidas de biomasa, glucosa residual, actividad poligalacturonasa y contenido de O2 y CO2 de los gases a la salida del reactor. La velocidad específica de crecimiento máxima (μm) de W. anomalus fue de 0,337 h -1 y el rendimiento de biomasa producida (Yx/s) de 0,401 gx/gs. Al finalizar el cultivo, la actividad PG en el sobrenadante fue de PG de ~ 83,7 UE/ml. La actividad específica y la productividad obtenidas fueron de ~ 1,91. 10 4 UE/gx y ~ 9.301 UE/l.h, respectivamente. El cociente respiratorio fue cercano a 1 durante el proceso fermentativo. No se formó ningún otro producto, además de biomasa y CO2. El cultivo por lote resultó ser una buena alternativa para la producción de PG a partir de W. anomalus, obteniéndose un extracto con elevada actividad enzimática, en un medio de cultivo sintético y de bajo costo.Palabras clave: Wickerhamomyces anomalus, poligalacturonasa, cultivo por lote, parámetros estequiométricos, parámetros cinéticos. AbstractWickerhamomyces anomalus, a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O2 and CO2 content of outlet gases from the reactor. The maximum specific growth rate (μm) of W. anomalus was 0.337 h -1 and the biomass yield (Yx/s) was 0.40 gx/gs. At the end of the culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/gx and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO2 was detected. Batch culture could be an adequate alternativ...

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Section: Resultados Y Discusiónunclassified

“…Wickerhamomyces anomalus, aislada a partir de frutas cítricas en la provincia de Misiones, Argentina (Martos et al, 2013a).…”

Section: Microorganismounclassified

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Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la producción de una poligalacturonasa

Martos

Butiuk

Rojas

et al. 2014

Rev. Colomb. Biotecnol.

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“…anomalus, isolated from the citrus fruit peels in the province of Misiones, Argentina (Martos et al 2013a) was used in this study.…”

Section: Microorganismmentioning

confidence: 99%

“…For such purposes, only the intercellular cementing material that holds together the cells (protopectin) and some portion of primary plant cell walls should be removed without damage to adjacent secondary cell walls, to help avoid cell lysis, keeping the nutritional properties of food (Costa et al 2007). A pectinolytic yeast strain was isolated from the citrus fruit peels in the province of Misiones, Argentina and it was identified as Wickerhamomyces anomalus, re-classification of the species Pichia anomala (Kurtzman 2008;Martos et al 2013a). This wild yeast strain, grown on a yeast nitrogen base medium with glucose and citrus pectin produced an endo-PG with maceration activity of vegetable tissues.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus

Martos

Butiuk

Rojas

et al. 2014

Braz. arch. biol. technol.

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Improved biomass and protein production in solid-state cultures of an Aspergillus sojae strain harboring the Vitreoscilla hemoglobin

Mora-Lugo

Madrigal

Yelemane

et al. 2015

Appl Microbiol Biotechnol

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The biotechnological value of Aspergillus sojae ATCC 20235 (A. sojae) for production of pectinases in solid-state fermentation (SSF) has been demonstrated recently. However, a common drawback of fungal solid-state cultures is the poor diffusion of oxygen into the fungi that limits its growth and biological productivity. The bacterial Vitreoscilla hemoglobin (VHb) has favored the metabolism and productivities of various bacterial and yeast strains besides alleviating hypoxic conditions of its native host, but the use of VHb in filamentous fungi still remains poor explored. Based on the known effects of VHb, this study assessed its applicability to improve A. sojae performance in SSF. The VHb gene (vgb) under control of the constitutive Aspergillus nidulants gpdA promoter was introduced into the genome of A. sojae by Agrobacterium-mediated transformation. Successful fungal transformants were identified by fluorescence microscopy and polymerase chain reaction (PCR) analyses. In solid-state cultures, the content of protease, exo-polygalacturonase (exo-PG), and exo-polymethylgalacturonase (exo-PMG) of the transformed fungus (A. sojae vgb+) improved were 26, 60, and 44 % higher, respectively, in comparison to its parental strain (A. sojae wt). Similarly, biomass content was also 1.3 times higher in the transformant strain. No significant difference was observed in endo-polygalacturonase (endo-PG) content between both fungal strains, suggesting dissimilar effects of VHb towards different enzymatic productions. Overall, our results show that biomass, protease, and exo-pectinase content of A. sojae in SSF can be improved by transformation with VHb.

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Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots

Cited by 20 publications

References 30 publications

Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la producción de una poligalacturonasa

Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la producción de una poligalacturonasa

Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus

Improved biomass and protein production in solid-state cultures of an Aspergillus sojae strain harboring the Vitreoscilla hemoglobin

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