“…However, on a scaffoldlevel, previous investigations have demonstrated that scaffold volume fraction can influence cell infiltration: the larger the pore size (lower volume fraction) the more the cells disperse throughout the scaffold due the increased fluid movement [Byrne et al, 2008;Tierney et al, 2008]; though interestingly without fluid influences, and when contact guidance is measured (cell motility along scaffold struts), cells migrate less in larger pores (151µm to 96µm) . Similarly, biosynthesis and degradation rates have also been shown to vary according to scaffold properties, such as cross-link densities and stiffness, which in part is related to relative density [Chevallay et al, 2000;Lee et al, 2001;Petite et al, 1994;Powell et al, 2006;Vickers et al, 2006;Weadcock et al, 1996]. Thus, it is likely the regional variances do impact on cell activity and tissue growth.…”