Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

“…Currently, extensive efforts are being made to develop protein-based biomaterials for biomedical applications [1,2]. Having similar macromolecular structures to naturally occurring proteins in tissues and organs, protein-based biomaterials have the potential to provide biological functionality and can be degraded by natural enzymes and metabolized by physiological mechanisms [3].…”

Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons

“…Collagen alignment along the isoelectric point occurred after supplying 20 VDC to the electrochemical cell equipped with a 1 megaohm resistor. Immediately after alignment, aligned collagen threads were incubated at 37 1C in 50 mL of 1X PBS (Uquillas et al, 2011) (Sigma-Aldrich, St. Louis, MO) for 6 h. After phosphate incubation, the aligned collagen threads were immersed in 20 mL of pure isopropyl alcohol (Zeugolis et al, 2008b) for 6 h. The threads were then washed with copious amounts of ultrapure water for 20 min. The isopropyl alcohol-free threads were laid over a nonsticking surface and fixed at both ends with tape.…”

Collagen‐Based Biomaterials for Regenerative Medicine

“…When hydrogel contraction is restrained, fibroblasts are under tension. Then, they differentiate into myofibroblasts and adopt a synthetic phenotype [32,33]. Another example is the influence of the biomaterial stiffness on chondrocyte phenotype.…”