Isolation and characterization of mesenchymal stem cells from the yolk sacs of bovine embryos

Mançanares, Celina Almeida Furlanetto; Oliveira, Vanessa Cristina de; Oliveira, Lilian Jesus de; Carvalho, Ana Flávia de; Sampaio, Rafael Vilar; Mançanares, Ana Carolina Furlanetto; Souza, Aline Fernanda de; Perecin, Felipe; Meirelles, Flávio Vieira; Miglino, María Angélica; Ambrósio, Carlos Eduardo

doi:10.1016/j.theriogenology.2015.05.031

Cited by 23 publications

(28 citation statements)

References 35 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, the third, the cells need to differentiate between osteoblasts, chondroblasts and adipocytes. Additionally, studies confirm that in culture and conditions for culturing, MSCs exhibit fibroblastoid morphology (Casteilla et al 2011, Tharasanit et al 2011, Mançanares et al 2015.…”

Section: Mesenchymal Stem Cellmentioning

confidence: 73%

Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Cell therapy represents an old therapeutic practice initiated with the transfusion of whole blood in different clinical situations. There is now a breakthrough in the study of multipotent stem cell therapy because of its functionality in regeneration of tissues, which promotes attention of the scientific community. Bone marrow is one of the main sources of multipotent stem cells, composed by hematopoietic stem cells responsible for the renewal of the cellular components of the blood, and mesenchymal stem cells that aid in the regeneration of tissues. These cells have a strong potential for the treatment of several diseases, due their main characteristics such as high plasticity, capacity for self-renewal and immunomodulation. Although, there are many studies that show good results with the use of cell therapy as a form of treatment for several diseases, some studies still show inconclusive or unsatisfactory results. Therefore, the objective of this study was to review the application of bone marrow stem cells in the canine model since improvements on the knowledge of the technique are necessary to enable its applicability with safety and efficacy.

show abstract

Section: Mesenchymal Stem Cellmentioning

confidence: 73%

Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

View full text Add to dashboard Cite

show abstract

“…The samples were then dried in an oven for two hours, metallized in a gold bath with a metallizer (Emitech K 550) and examined under a scanning electron microscope (Leo—435 VP Zeiss). The transmission electron microscopy (TEM) protocol has been previously established [ 45 ]. Briefly, the EGCs were fixed in 2.5% glutaraldehyde diluted in PBS (pH 7.4–0.1 M) for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of male canine germ cell development

et al. 2018

View full text Add to dashboard Cite

Primordial germ cells (PGCs) are precursors of gametes that can generate new individuals throughout life in both males and females. Additionally, PGCs have been shown to differentiate into embryonic germ cells (EGCs) after in vitro culture. Most studies investigating germinative cells have been performed in rodents and humans but not dogs (Canis lupus familiaris). Here, we elucidated the dynamics of the expression of pluripotent (POU5F1 and NANOG), germline (DDX4, DAZL and DPPA3), and epigenetic (5mC, 5hmC, H3K27me3 and H3K9me2) markers that are important for the development of male canine germ cells during the early (22–30 days post-fertilization (dpf)), middle (35–40 dpf) and late (45–50 dpf) gestational periods. We performed sex genotype characterization, immunofluorescence, immunohistochemistry, and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) analyses. Furthermore, in a preliminary study, we evaluated the capacity of canine embryo PGCs (30 dpf) to differentiate into EGCs. To confirm the canine EGCs phenotype, we performed alkaline phosphatase detection, immunohistochemistry, electron and transmission scanning microscopy and RT-qPCR analyses. The PGCs were positive for POU5F1 and H3K27me3 during all assessed developmental periods, including all periods between the gonadal tissue stage and foetal testes development. The number of NANOG, DDX4, DAZL, DPPA3 and 5mC-positive cells increased along with the developing cords from 35–50 dpf. Moreover, our results demonstrate the feasibility of inducing canine PGCs into putative EGCs that present pluripotent markers, such as POU5F1 and the NANOG gene, and exhibit reduced expression of germinative genes and increased expression of H3K27me3. This study provides new insight into male germ cell development mechanisms in dogs.

show abstract

“…The mesenchymal stem cell (MSC) is a type of multipotent stem cell present in most adult tissues, a heterogeneous cell that plays a key role in the development and renew of organs (Klein, 2016). Several reports have shown that MSCs are commonly isolated and characterized from adipose tissue, bone marrow, umbilical cord, liver, as well a fetal attachments such as yolk sac and amnion (Seo et al, 2009;Uranio et al, 2011;Wenceslau et al, 2011;Reich et al, 2012;Mançanares et al, 2015). This adaptability and easy access make this stem cell a great candidate for use in transplants and therapies.…”

Section: Stem Cells Lineagesmentioning

confidence: 99%

“…According to the International Society of Cell Therapy the criteria for the characterization of human MSC are adherence to the plastic, positive expression for the surface markers CD105, CD73 and CD90, negative expression for the markers CD45, CD34, CD14 or CD11b, CD79a or CD19 and the HLA-DR surface molecules, capability to differentiate (osteoblasts, adipocytes and chondrocytes) in vitro (Dominici et al, 2006). According to Casteilla et al (2011), Tharasanit et al 2011), Mançanares et al (2015), MSCs exhibit fibroblastoid morphology in culture.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Potential application of aminiotic stem cells in veterinary medicine

et al. 2019

View full text Add to dashboard Cite

In regenerative medicine stem cell biology has become one of the most interesting and more often studied subject. The amniotic membrane is the innermost layer of the fetal membranes and is considered a potential tool to treat many pathologies. It is used because it can be collected from discarded fetal material and is a rich source of stem cells with high proliferation and plasticity ratio capable of proliferating and differentiate in vitro. We propose to elucidate the characteristics and potencial clinical application of cells derived of amniotic membrane in veterinary medicine.

show abstract

Isolation and characterization of mesenchymal stem cells from the yolk sacs of bovine embryos

Cited by 23 publications

References 35 publications

Bone marrow stem cell applied in the canine veterinary clinics

Bone marrow stem cell applied in the canine veterinary clinics

Dynamics of male canine germ cell development

Potential application of aminiotic stem cells in veterinary medicine

Contact Info

Product

Resources

About