Potential application of aminiotic stem cells in veterinary medicine

Ambrósio, Carlos Eduardo; Orlandin, Jéssica Rodrigues; Oliveira, Vanessa Cristina de; Motta, Lina Castelo Branco; Pinto, Priscilla Avelino Ferreira; Pereira, Vitória Mattos; Padoveze, Letícia Ribeiro; Karam, Rafael Garcia; Pinheiro, Alessandra de Oliveira

doi:10.21451/1984-3143-ar2018-0124

Cited by 13 publications

(9 citation statements)

References 75 publications

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“…Therefore, the diversity of results on the immunomodulation of MSCs may be directly related to the tissue being studied or the mechanisms inherent to each species, 46 such as in dogs. 47…”

Section: Discussionmentioning

confidence: 99%

<p>Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells</p>

Pinheiro¹,

Lara²,

Souza³

et al. 2020

SCCAA

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Purpose: Amniotic membrane stem cells have a high capacity of proliferation, cell expansion, and plasticity, as well as immunomodulatory properties that contribute to maternal-fetal tolerance. Owing to the lack of research on human amniotic membrane at different gestational stages, the canine model is considered ideal because of its genetic and physiological similarities. We aimed to characterize the canine amniotic membrane (CAM) cell lineage in different gestational stages and evaluate the expression of immunomodulatory genes. Materials and Methods: Twenty CAMs from early (20-30 days) (n=7), mid-(31-45 days) (n=7), and late gestation (46-63 days) (n=6) stages were studied. The cell features were assessed by cell viability tests, growth curve, colony-forming units, in vitro differentiation, cell labeling for different immunophenotypes, and pluripotent potential markers. The cells were subjected to RT-PCR and qPCR analysis to determine the expression of IDO, HGF, EGF, PGE2, and IL-10 genes. Results: CAM cells exhibited a fibroblastoid morphology and adherence to plastic with an average cell viability of 78.5%. The growth curve indicated a growth peak in the second passage and we obtained an average of 138.2 colonies. Osteogenic, chondrogenic, and adipogenic lineages were confirmed by in vitro differentiation assays. Cellular immunophenotyping experiments confirmed the presence of positive mesenchymal markers (CD90 and CD105) and the low or negative expression of hematopoietic markers (CD45 and CD34). Qualitative analysis of the immunomodulatory functions indicated the expression of the IDO, HGF, EGF5, and PGE2 genes. When stimulated by interferon-gamma, CAM cells exhibited higher IDO levels throughout gestation. Conclusion: The CAMs from different gestational stages presented features consistent with mesenchymal stem cell lineage; better results were observed during the late gestation stage. Therefore, the gestational stage is a key factor that may influence the functionality of therapies when using fetal membrane tissues from different periods of pregnancy.

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“…Therefore, the diversity of results on the immunomodulation of MSCs may be directly related to the tissue being studied or the mechanisms inherent to each species, 46 such as in dogs. 47…”

Section: Discussionmentioning

confidence: 99%

<p>Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells</p>

Pinheiro¹,

Lara²,

Souza³

et al. 2020

SCCAA

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“…Initially, MSCs were derived from bone marrow; however, it is known that this cell population is small and decreases with age. Placental attachments, such as the chorion and the amniotic membrane serve as well-characterized alternative sources of MSCs (Ambrósio et al, 2020;Jones & Jauniaux, 1995); however, few studies have investigated the culture of MSCs derived from the YS (Jang et al, 2013).…”

Section: Ys-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

Mammalian yolk sac - an alternative source of stem cells

Pinto

Pereira²,

Motta³

et al. 2021

Braz. J. Vet. Med.

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Fetal tissue-derived stem cells have been the subject of several studies. Among them, the yolk sac (YS) nourishes the embryo, synthesizes proteins and vitamins, and is the first hematopoietic site of the embryo. Stem cells derived from the YS can benefit several animal species as therapies aimed at tissue regeneration and immunological diseases. This review aims to describe the development, function, and possible dysfunctions of YS in different species, and consolidate the findings presented in the literature on the isolation, culture, and application of YS-derived stem cells from different mammals in regenerative medicine. Hematopoietic and mesenchymal stem cells have been previously investigated in the YS of different mammals; however, the culture media and isolation protocols differ between species. To date, no studies have been conducted using stem cells derived from the YS for cell therapy. Nevertheless, several domestic mammals have shown cellular markers characteristic of mesenchymal stem cells derived from the YS.

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“…9 Thus, cell therapy has provided hope in the treatment of spinal cord injuries. [10][11][12][13][14][15][16][17][18] The placenta performs primordial functions in the embryonic development of mammals since it is responsible for the protection, nutrition and oxygenation of fetuses. It consists of four membranes: the chorion, allantois, yolk sac and amnion.…”

Section: Introductionmentioning

confidence: 99%

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

Orlandin

Gomes

Leandro

et al. 2021

SCCAA

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Introduction: Intervertebral disc diseases (IVDD) represent the majority of neurological attendance and responsible for the most cases of paralysis in dogs. Treatments currently used do not show satisfactory results in patients with more severe and chronic neurological manifestations. Methods: To promote nerve and muscular recovery, as well as improve quality of life, we aimed to create a double-blind test method, associating spinal decompression surgery and allogeneic transplantation of amniotic membrane-derived stem cells (AMSCs) in dogs with chronic IVDD. Cells were characterized as fetal mesenchymal cells and safe for application. Eight animals completed the experiment: stem cell applications were made in four animals that had previously undergone an unsuccessful surgical procedure ("SC group", n = 4); two animals were submitted to surgery, followed by applications of stem cells ("Surgery + SC", n = 2); two other animals were submitted to surgery, followed by the application of saline solution ("Surgery + placebo", n = 2). During the surgical procedure, a topical application was performed on the lesion and after fifteen and forty-five days another two applications were made via epidural. Animals were monitored biweekly and reassessed three months after surgery, by functional tests and magnetic resonance exams. Results: Some animals presented significant neurological improvement, such as the recovery of nociception and ability to remain on station. Despite the need further studies, until the present moment, cell therapy has been feasible and has no harmful effects on animals. Conclusion:The protocol of preclinical trial showed the association with decompressive surgery and cell transplantation in dogs with thoracolumbar IVDD proved feasible, and it was possible to observe neurological improvement after treatment. No tissue improvement through MRI was found. The double-blind test guaranteed reliability of the evaluations and results obtained that, even with a small sample size, generated satisfactory results for the animals and owners.

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Potential application of aminiotic stem cells in veterinary medicine

Cited by 13 publications

References 75 publications

<p>Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells</p>

<p>Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells</p>

Mammalian yolk sac - an alternative source of stem cells

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

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