Implications of Sertoli cell induced germ cell apoptosis to testicular pathology

Murphy, Caitlin J.; Richburg, John H.

doi:10.4161/21565562.2014.979110

Cited by 44 publications

(26 citation statements)

References 59 publications

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“…25,29 With a more extended period of dosing reduces the specificity of the pattern of spermatogenic disturbance as the tubules become depleted of more germ cells. Whether spontaneous or induced, death germ cells appear to occur predominantly through apoptosis, are tightly regulated by Sertoli cells 23,31 . Many of dying cells do not have the classic morphologic appearance of apoptotic cells.…”

Section: Figurementioning

confidence: 99%

Antifertility effect of betel nut (Areca catechu L) in male rat

Rahman

Purwakanthi²,

Dewi³

2020

medisians

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Background: Betel nut (Areca catechu L) are traditional beverages in Jambi. Betel nut high dose has a cytotoxic effect on testis and other organs—limited data about the effect of betel nut as male antifertility.Purposes: This study aimed to determine the effect of infertility on betel nut use at a dose of 50 mg/kg body weight (BW) in male rats. Methods: Ten male Sprague Dawney rats that had passed the fertility test, aged 2-3 months and weighing 150-200 grams ware used in this study. The rat was grouped into two groups randomly. Group 1, as the negative control, were received aqua dest, and group 2 was treated by betel nut with dose 50 mg/kg BW for 35 days. When terminated, testis weight weighed using micro scales. Blood collected for measuring testosterone levels. Histopathology assessment of testis used Hematoxylin Eosin Staining and sperm counting from cauda epididymis.Results: The weight of testis in the group received betel nut was lower than the control group. The histopathology of testis showed shrunk, reduced the diameter of seminiferous tubules, and like cytolytic lesions in the germinal layer—the total sperm number and progressive sperms also lower than the control group (p<0.05). There was no abnormality in Leydig cell and interstitium tissue. Male rats after treatment could not impregnate the female rats.Conclusion: Betel nut at dose 50 mg/kg BW have antifertility activity in male rats.

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Section: Figurementioning

confidence: 99%

Antifertility effect of betel nut (Areca catechu L) in male rat

Rahman

Purwakanthi²,

Dewi³

2020

medisians

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“…Studies have shown that the Sertoli cell is one of the primary targets of testis injury following exposure of rats to toxicants including PFOS, phthalates, bisphenol, cadmium and others [31-35]. As such, investigators have sought to use primary Sertoli cells isolated from rat testes and cultured in vitro as a model system to study the biology of toxicant-induced Sertoli cell injury [36-39] (Table 1).…”

Section: Role Of Fak In Pfos-mediated Sertoli Cell Injurymentioning

confidence: 99%

Mechanistic Insights into PFOS-Mediated Sertoli Cell Injury

Mao

Mruk

Lian

et al. 2018

Trends in Molecular Medicine

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Studies have proven that per- and polyfluoroalkyl substances are harmful to humans, most notably perfluorooctanesulfonate (PFOS). PFOS induces rapid disorganization of actin- and microtubule (MT)-based cytoskeletons in primary cultures of rodent and human Sertoli cells, perturbing Sertoli cell gap junction communication, thereby prohibiting Sertoli cells from maintaining cellular homeostasis in the seminiferous epithelium to support spermatogenesis. PFOS perturbs several signaling proteins/pathways, such as FAK and mTORC1/rpS6/Akt1/2. The use of either an activator of Akt1/2 or overexpression of a phosphomimetic (and constitutively active) mutant of FAK or connexin 43 has demonstrated that such treatment blocks PFOS-induced Sertoli cell injury by preventing actin- and MT-based cytoskeletal disorganization. These findings thus illustrate an approach to manage PFOS-induced reproductive dysfunction.

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“…Robeva et al [64] have also reported, through clinical trials, that Sertoli cell function is suppressed in MetS. As Sertoli cells support and provide nutrition for the developing germ cells and are absolutely necessary for proper germ cell development and viability [55], Sertoli cell dysfunction reduces supportive capacity and impairs spermatogenesis and fertility [85]. Sertoli cell changes may also explain the abnormal presence of spermatids near the basement membrane as reported by Russell et al [65] who suggested spermatids' phagocytosis by Sertoli cells and, then, failure of release.…”

Section: Discussionmentioning

confidence: 99%

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

El-Mehi

Faried

2020

Folia Morphol

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Background: This work was designed to clarify, for the first time up to our knowledge, the effects of high fructose diet (HFrD) induced metabolic syndrome (MetS) on the pituitarygonadal axis of adolescent male rats continuing through adulthood period and to evaluate the possible role of ginger extract in ameliorating these effects. Materials and methods:Forty 4-week-old male albino rats, treated for 8 weeks, were randomly divided into 4 equal groups; control(fed standard diet), ginger extract treated (500 mg/kg once daily orally by gavage), HFrD induced MetS (fed a diet containing 60% fructose), HFrD and ginger groups. The assessment methods included biochemical, histological and immunohistochemical studies.Results: HFrD fed rats exhibited a picture similar to MetS in the form of increased body weight and serum levels of glucose and insulin with an elevated HOMA-IR reflecting insulin resistance as well as dyslipidemia. Lipid peroxidation (increased MDA) and oxidative stress (decreased SOD) were implicated in this syndrome. This group exhibited a significant decrease of testicular weight and the levels of reproductive hormones (LH, FSH & testosterone). The pituitary gonadotrophs showed electron dense nuclei, large cytoplasmic vacuoles, destructed organelles in addition to decreased number of secretory granules. Furthermore, testicular specimens presented marked alterations. There were disorganized shrunken tubules with irregular basement membranes, reduced germinal epithelial thickness, vacuolations and degenerated mitochondria in the spermatogenic cells.Beclin1 and PCNA immunoexpressions were significantly downregulated with HFrD induced MetS. Ginger extract supplementation proved to be a potent protective agent against these harmful effects through its antioxidant, hypoglycemic, insulinotropic, androgenic and hypolipidemic effects in addition to its ability to induce testicular autophagy. Conclusions: Consumption of HFrD induced MetS and proved to have harmful effects on the pituitarytesticular axis. Moreover, this work provided a new insight into the possible use of ginger extract to alleviate the main features of MetS and protect the pituitarygonadal axis from the damaging effects of HFrD induced MetS.

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Implications of Sertoli cell induced germ cell apoptosis to testicular pathology

Cited by 44 publications

References 59 publications

Antifertility effect of betel nut (Areca catechu L) in male rat

Antifertility effect of betel nut (Areca catechu L) in male rat

Mechanistic Insights into PFOS-Mediated Sertoli Cell Injury

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

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