GENETIC DIVERSITY AMONG SUGARCANE (Saccharum officinarum L.) GENOTYPES AS SHOWN BY RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD)

Hapsoro, Dwi; Warganegara, Hayane Adeline; Utomo, Setyo Dwi; Sriyani, Nanik; Yusnita, Yusnita

doi:10.17503/agrivita-2015-37-3-p247-257

Cited by 6 publications

(6 citation statements)

References 17 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular markers that frequently used to assess genetic diversity are random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. RAPD markers are more effective and efficient to determine the recombinant variability than morphological markers in assessing genetic diversity (Paul, Bandyophadyay, Acharyya, & Raychaudhuri, 2010;Hapsoro, Warganegara, Utomo, Sriyani, & Yusnita). RAPD markers also become a potential tool to identify genetic differences among varieties (Biswas, Akhond, Al-Amin, Khatun, & Kabir, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Herison

Sutjahjo

Sulastrini³

et al. 2018

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

Genetic diversity is the most important aspect in tomato breeding activities. Better assessment on the diversity of the collected accessions will come up with better result of the cultivar development. This study aimed at analyzing the genetic diversity of 27 tomato accessions by morphological and molecular markers. Twenty seven accessions collected from various regions of Indonesia were planted in the field and evaluated for their morphological traits, and RAPD analyzed for their molecular markers. The UPGMA clustering analyzes, elaborating the combination of morphological and molecular data, indicated that the tomato accessions could be grouped into 5 major groups with 70 % genetic similarity levels. Current study indicated that although many accessions came from different locations, they congregated into the same group. Cherry, Kudamati 1 and Lombok 3 were the farthest genetic distant accessions to the others. Those three genotypes will be the most valuable accessions, when they were crossed with other accessions, for designing a prospective breeding program in the future.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Herison

Sutjahjo

Sulastrini³

et al. 2018

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

show abstract

“…Many study also reported that in many sugarcane crossing program around the world, the parents that used for crosses originated from inter-and intraspecific hybridization and its progenies. They results progenies with the same and share genetic makeup with parents (Stevenson, 1965;Zhang et al, 2001;Perera et al, 2012;Filho et al, 2010;Pan et al, 2003;Hapsoro et al, 2015;Chen et al, 2017). In contrast to previous result, in crosses between Erianthus sp.…”

Section: Discussionmentioning

confidence: 87%

Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

Murianingrum¹,

Taryono

Wulandari

2019

ipas

View full text Add to dashboard Cite

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.

show abstract

“…4. Hapsoro, Warganegara, Utomo, Sriyani, & Yusnita (2015), also used RAPD markers on some sugarcane genotypes from Australia, Africa, America and Asia, found genetic similarities ranging from 17-97 % with an average genetic similarity of around 57 %. Fig.…”

Section: Fig 3 Electrophoresis Results Using Rapd Opn11 Primersmentioning

confidence: 99%

Morphological Diversity and Molecular RAPD Markers of Sugarcane Mutane (Saccharum officinarum L.) in Inundation Tolerance

Avivi

Suliswanto²,

Restanto³

et al. 2019

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7-56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.

show abstract

GENETIC DIVERSITY AMONG SUGARCANE (Saccharum officinarum L.) GENOTYPES AS SHOWN BY RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD)

Cited by 6 publications

References 17 publications

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

Morphological Diversity and Molecular RAPD Markers of Sugarcane Mutane (Saccharum officinarum L.) in Inundation Tolerance

Contact Info

Product

Resources

About