<p><em></em><em>Identification of Indonesia sugarcane varieties tolerant to waterlogging has not been done extensively. Information on varieties tolerant to waterlogging is required for seedling establishment in waterlogged areas. The purpose of this research was to identify the sugarcane varieties responses to several duration levels of waterlogging at seedling stage. The planting materials used were collection of Sugar Factory Semboro including VMC 76-16, BL, PS 862, PS 864, and PS 881. This research was arranged in randomized complete block design (RCBD) with two factors and three replications. The first factor was 5 varieties; the second factor was waterlogging treatment with four levels, i.e. without waterlogging, 2, 4, and 6 weeks of waterlogging in the bucket. The results showed different level of tolerance to water logging among the varities. PS 881 and VMC 76-16 varieties were the most tolerant to waterlogging supported by its ability to maintain plant height, root volume, root and shoot dry weight after been waterlogged for 6 weeks. These varieties were also able to establish aerenchyme tissue and increased the stomatal density. PS 862 was the least tolerant variety to waterlogging. <br /><br />Keywords: aerenchyme, morphology, stomatal density, waterlogging, water stress</em></p>
Indicator of coffee resistance to leaf rust attack (Hemileia vastatrix) is needed to select superior coffee plants resistant to biotic stress. This study aims to find the relationship between the content of secondary metabolites and the intensity of leaf rust attack, so that it becomes a reference in the selection of future coffee plants. The experimental design used was a completely randomized design (CRD) with several coffee clones as a treatment. This test tested 5 Arabica coffee clones consisting of Komasti, Maragogik, Usda, Andong sari, and HDT clones. Each consists of 3 replications, and each replication consists of 2 sample plants. Observations were made by observing the intensity of the attack, and plant metabolites such as phenolic content, flavonoids and antioxidant activity were observed when the leaves had been attacked by Hemileia vastatrix. Correlation of leaf rust attack levels with phenolic content, flavonoids and antioxidant activity showed a relationship between each observation variable. Flavonoid content in certain conditions can be used as an indicator to get Arabica coffee plants that are resistant to the attack of leaf rust.
The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7-56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
The aim of this research was to obtain the resistant plant toward waterlogging stress. The research used complete randomized block design (RBD) with two factors and three replications. The first factors of sugarcane variety are PS 8845, VMC 7616, BL, VMC 86550, PSJK 922 and PS 864. The second factor is the treatments of waterlogging on the level of treatment, that is, without waterlogging, 6, 9, and 12 weeks of waterlogging in bucket. The research results showed that the differences in resistance response of several tested varieties were seen in parameters of number of leaves, number of yellowing leaves, fresh weight of plant, plant height, stem diameter, root length, root’s branch, number of tillers, chlorophyll content, the rate of photosynthesis, stomatal density, shoot leaf size, and leaf angle. Quantitative data character were analysed using ANOVA and DMRT. The conclusion of this research were that PS 8845 and VMC 7616 variety can be recommended as a variety that is resistant to water logging stress. While variety PSJK 922 showed the worst response in condition of waterlogging stress.
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