Genetic differentiation of dengue vector Aedes aegypti in the small geographical scale of Banyumas District, Indonesia based on Cytochrome Oxidase I

Abstract

Abstract. Mohammed MA, Nuryanto A, Kusmintarsih ES. 2021. Genetic differentiation of dengue vector Aedes aegypti in the small geographical scale of Banyumas District, Indonesia based on Cytochrome Oxidase I. Biodiversitas 22: 675-683. Aedes aegypti (Linnaeus, 1762) is a major vector of arboviruses. Currently, Ae. aegypti is spreading throughout the Banyumas District, Central Java, Indonesia and there is little information regarding the genetic variation of this species, yet the information is essential to develop effective vector control measures. The aims of this study was to evaluate the genetic differentiation of Ae. aegypti populations in South Purwokerto and Cilongok subdistricts. Mosquito larvae were collected using oviposition traps inside houses in South Purwokerto and Cilongok (Jastaba village). Twenty larvae of Ae. aegypti, ten from each location were identified and used for genetic analysis. Analysis of mitochondrial cytochrome Oxidase I (mtCOI) produced around 600 bp fragments, detecting ten haplotypes. The haplotype diversity and nucleotide diversity indices were higher in South Purwokerto samples. Tajima and Fu’s statistics indicated that the sampled populations were in genetic equilibrium. Indices of demographic history were fit for a spatial expansion model. Higher genetic variation was observed within the population compared to between the populations. Furthermore, strong genetic difference was detected between the two populations, with highly significant FS (p < 0.0001). Weak haplotype sharing occurred between the two populations assuming that the gene flow was facilitated by human transportation. All haplotypes were a cluster of single clades and closely related to haplotypes generated from previous studies in Central Java.

DNA barcoding of crustacean larvae with two new records of Caridina gracilipes and Ptychognathus altimanus in the Western Region of Segara Anakan lagoon in Indonesia

“…This result is consistent with previous decisions on the taxonomic status of the samples, where they showed similar traits as the reference conspecific in the databases. Previous studies reported that a 3% genetic divergence is widely used in animal barcoding as a species border (Hubert et al 2012;Ko et al 2013;Mohammed et al 2021;Riani et al 2021). Several studies have also explored various thresholds as species border during molecular identification from less than 1% to 5% (Cote et al 2013;Aguilar et al 2017: Ha et al 2019Abdalwahhab et al 2020).…”

Morphological and molecular characterization of mole crab (Genus: Emerita) in the Cilacap coastlines of Indonesia, with particular focus on genetic diversity of Emerita sp. nov.

“…The negative symptoms and non-significant Fs (-1.580, p=0.147) could assume that the marker was neutral and indicated a population bottleneck (Table 5). The assumption was based on the fact that Fus' Fs values are believed to be more sensitive than Tajimas' D. According to Jong et al (2011) and Mohammed et al (2021), the sensitivity of Fus' Fs values because it is calculated based on nucleotide diversity. A similar phenomenon was also reported in fish (Setyaningrum et al 2022).…”

Current Evidence on Bioinformatics Role and Digital Forensics That Contribute to Forensic Science: Upcoming Threat

“…Molecular biology can be used for species identification on the molecular level, which cannot be done at the level of morphological characteristics [33], [34]. At the molecular level, genetic diversity was becoming a challenge for determining the genetic structure and its relationship between environment, human activity, and impact on urbanization [35], also conservation success [36].…”