Effect of bisphenol A on the cauda epididymis of adult male albino rats and the possible protective role of quercetin

Mazroa, Shireen A.

doi:10.1097/01.ehx.0000397468.63291.0c

Cited by 7 publications

(8 citation statements)

References 40 publications

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“…The statistical analysis in the present study revealed a significant reduction in the epithelial height and decrease of the tubular diameter in the treated group as compared with the control group. These results are in accordance with Mazroa [12] . The tubular lumen in the treated group was found to be either empty or contained cellular debris.…”

Section: Discussionsupporting

confidence: 93%

“…Excess connective tissue and collagen fibers were observed between epididymal tubules. Similar observation was reported in cauda epididymis [12] . Clear cells were markedly absent in the treated group.…”

Section: Discussionsupporting

confidence: 91%

“…The effect of BPA on the caput and cauda epididymis has been studied by El Gazzawy et al and Mazroa, respectively [11,12] . The current study is concerned with the effect on the corpus epididymis.…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress is a crucial factor to induce cell-cell dissociation [22] . BPA was reported to induce of oxidative stress in the cauda epididymis and other vital organs [12,24] . The wide intercellular spaces reflect a probable affection of the tight junction between the epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Bisphenol a on Corpus Epididymis and Chromosomal Pattern of Adult Rats

Hegazy¹,

Omar²,

Hussein³

et al. 2017

Zagazig University Medical Journal

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Introduction: The epididymis is a male accessory organ essential for male fertility. The biochemical and physiological consequences of genotoxicity which are of most concern are the effects on the reproductive health. Bisphenol A (BPA) is a chemical used in the production of polycarbonate plastics and epoxy resins. It is present in food and drink packages. It is considered an endocrine disrupting factor due to is estrogenic properties. Aim of the Work:The study aimed to demonstrate the possible changes in the corpus epididymis structure and chromosomal pattern following administration of BPA. Materials and Methods: Thirty-six adult male albino rats were used; and divided into three groups: Group Ι (control); each rat received 1mL corn oil once daily. Group ΙІ (BPA treated); each rat was given BPA in corn oil in a dose of 50 mg/kg orally once daily for 8 weeks. Group ΙΙІ (follow-up); the rats of this group were given the same dose for the same duration as group ΙІ then held for another 4 weeks without BPA administration. At the end of experimental period, blood was collected for serum testosterone assessment. Histological and immunohistochemical investigations were done on epididymis; and the femoral bone was used for cytogenetic studies. Results: Light microscopic examination of corpus epididymis in treated group revealed wide intercellular and intertubular spaces with inflammatory infiltration and blood vessels congestion. The tubules were empty or filled with cellular debris. The epithelial lining showed vaculation, darkly stained nuclei and stratification. The abnormal sperm forms and both the structural and numerical chromosomal aberrations were significantly increased. These parameters were improved in follow-up group but not reached the levels of control one. Conclusion: BPA administration could induce histopathological changes in corpus epididymis associated with genotoxic effects. These changes were partially reversible after stoppage of administration.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of Bisphenol a on Corpus Epididymis and Chromosomal Pattern of Adult Rats

Hegazy¹,

Omar²,

Hussein³

et al. 2017

Zagazig University Medical Journal

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“…Additionally, electron microscopic examination of the current study revealed increased lysosomes, dilated proliferated Golgi apparatus and rough endoplasmic reticulum. Similar results were observed during studying the effect of bisphenol A on the epididymis of adult rats [43] . These changes might be due to alterations in the secretory function of the cells induced by TCS.…”

Section: Discussionsupporting

confidence: 86%

Histological, immunohistochemical and biochemical study of the effect of triclosan and its withdrawal on cauda epididymis of adult albino rat

Elbakry

Ibrahim

2019

Egyptian Journal of Histology

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Background: Triclosan (TCS) is a bactericide used in many daily products. TCS potential for endocrine disruption is suspected to impact male reproductive system. Aim of the study: Is to evaluate the effect of TCS and its withdrawal on the cauda epididymis of adult albino rat using different histological and biochemical techniques. Materials & Methods: Twenty-one adult male albino rats were equally divided into 3 groups; control, TCS-treated (administered 200mg/kg/day for 8weeks) and TCS-withdrawal group (administered 200mg/kg/day for 8weeks then left without treatment for 8weeks). Serum testosterone was quantified and epididymal specimens were processed for light and electron microscopic examination. Immunohistochemical staining against androgen receptor was performed. Results: TCS-treated group revealed a significant decrease in serum testosterone. Principal cells showed vacuoles compressing their nuclei, some pyknotic nuclei and sparse stereocilia. Many adluminal halo cells were observed. The epithelial lining depicted focal areas of stratification and areas of discontinuity. Widened interstitium inbetween the ducts with cellular infiltrations and some congested blood vessels were observed. Mallory-trichrome staining revealed prominent collagen fibers surrounding the epididymal ducts and blood vessels and an enhanced Periodic-Acid-Schiff reaction in the thickened basal lamina coupled to a diminished Periodic-Acid-Schiff reaction in the apical parts of the epididymal epithelial cells. A weak nuclear androgen receptor immunoreaction in the epididymal epithelial lining of the ducts was detected. Ultrastructural examination depicted principal cells with irregular nuclei, vacuolated cytoplasm with many lysosomes, dilated Golgi apparatus and rough endoplasmic reticulum. Irregular apical cytoplasmic projections and sparse stereocilia were observed. Clear cells showed some cytoplasmic projections, large apical vacuoles and few lipid droplets. Migrating halo cells with vacuolated cytoplasm were observed. Withdrawal group showed a near normal histology. Conclusion: TCS has extremely affected the epididymal lining through acting as an endocrine disruptor. Withdrawal of TCS exerted a satisfactory outcome.

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Zinc deficient diet increases the toxicity of bisphenol A in rat testis

Sahu

Charaya

Singla

et al. 2020

J Biochem & Molecular Tox

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Zinc (Zn) plays an important role in maintaining the process of spermatogenesis and reproductive health. Bisphenol A (BPA), an endocrine disrupting chemical is known to be a reproductive toxicant in different animal models. The present study was designed to study the effect of two of the utmost determinative factors (Zn deficient condition and influence of toxicant BPA) on germ cell growth and overall male reproductive health in the testis, epididymis, and sperm using (a) biochemical, (b) antioxidant, (c) cellular damage, (d) apoptosis, and (e) protein expression measurements. Rats were divided into Control (normal feed and water), BPA (100 mg/kg/d), zinc deficient diet (ZDD; fed with ZDD), and BPA + ZDD for 8 weeks. Body and organ weights, sperm motility and counts, and sperm head morphology were evaluated. The histology of testes, epididymides, and prostate was investigated. Testicular deoxyribonucleic acid (DNA) damage was evaluated by Halo and Comet assay, apoptosis of sperm and testes were quantified by TUNEL assay. Serum protein electrophoretic patterns and testicular protein expressions such as Nrf-2, catalase, PCNA, and Keap1 were analyzed by Western blot analysis. The results showed that BPA significantly increased the testicular, epididymal, and prostrate toxicity in dietary Zn deficient condition due to testicular hypozincemia, hypogonadism, increased cellular and DNA damage, apoptosis, as well as perturbations in protein expression.

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Effect of bisphenol A on the cauda epididymis of adult male albino rats and the possible protective role of quercetin

Cited by 7 publications

References 40 publications

Effect of Bisphenol a on Corpus Epididymis and Chromosomal Pattern of Adult Rats

Effect of Bisphenol a on Corpus Epididymis and Chromosomal Pattern of Adult Rats

Histological, immunohistochemical and biochemical study of the effect of triclosan and its withdrawal on cauda epididymis of adult albino rat

Zinc deficient diet increases the toxicity of bisphenol A in rat testis

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