Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

Abstract

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

Evaluation of Harvest Age on Physicochemical Properties and Antioxidant Capacity of Butternut Pumpkin Flour

“…Biosintesis dan Tabel 2. Hasil analisis kimia terhadap tepung labu kuning madu pada berbagai umur panen (15,20,25, dab 30 hari setelah terbentuknya bakal buah) Table 2. The results of chemical analysis on maturity stage factor of honey pumpkin flour at various harvest age (15, 20, 25, Tabel 3.…”

Phenolics Contents, Xanthine Oxidoreductase Inhibitory Potential, Antibacterial and Antioxidant Activities of Cachrys libanotis L. Root Extracts

“…A correlation analysis was performed on total phenolics and flavonoids contents using Pearson's correlation coefficient. Pearson's correlation coefficient was positively high if 0.610 < r < 0.974 and negatively high if -0.610 < r < -0.974 33 . As expected, total phenolics content of CrE and fractions had strong positive correlation with flavonoids content (r = 0.893).…”

Antioxidant activity assay of white Turi (Sesbania grandiflora (L.) Pers.) extracts using DPPH radical scavenging method

“…This can be supported from previous studies conducted by Rohmah et al, (2018) on acetone extract of leaves and stems of white Turi (Sesbania grandiflora) have antioxidant power with IC 50 values of 56.5707 ppm and 54.2608 ppm. Besides, the type of yellow Turi, namely ethanol extract of yellow Turi leaf, has antioxidant power with an IC 50 value of 4.12 ppm (Fitriansyah et al, 2017). Likewise, Sesbania sesban leaves extracts with different polarity solvents (n-hexane extract, ethyl acetate extract and ethanol extract) can be categorized as very strong antioxidant.…”

“…The research of (Fitriansyah et al, 2017) showed that extracts of n-hexane, ethyl acetate, and ethanol of Sesbania sesban (L. Merr) leaves had an IC 50 DPPH antioxidant activity <50 μg / mL and could be classified as a very strong antioxidant. Sesbania Sesban ethanol extract has the highest total phenolic content (5.18 g GAE / 100 g) and the highest total flavonoid content (4.56 g QE / 100 g), while the highest total carotenoid content (4.56 g BE / 100 g) in n-hexane extract.…”