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Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

Objectives In this study, we aimed to determine the antioxidant activities of two varieties of sesame ( Sesamum indicum L.) seeds using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) method, and to investigate the correlation between total phenolic as well as flavonoid contents and antioxidant activities of the extracts. Methods The antioxidant activities were determined using DPPH, ABTS, and FRAP assays, and the total phenolic content (TPC) and total flavonoid content (TFC) were measured by ultraviolet (UV)-Vis spectrophotometry. The correlation between total phenolic and flavonoid contents and DPPH IC 50 , ABTS IC 50, and FRAP EC 50 values was analyzed by Pearson's method. Results The IC 50 DPPH values of all sesame seed extracts were in the range of 8.88–44.21 μg/mL and IC 50 ABTS values were in the range of 24.91–141.19 μg/mL. EC 50 FRAP value ranged from 222.40 to 872.57 μg/mL. The highest TPC of 1.57 g gallic acid equivalent (GAE)/100 g was observed in ethanolic extract of black sesame seed, while the highest TFC of 4.29 g quercetin equivalent (QE)/100 g was observed in ethyl acetate extract of white sesame seeds. The TPC in black sesame seed extract was significantly negative correlated with IC 50 ABTS value (r = −0.828, p < 0.01) and EC 50 FRAP value (r = −0.976, p < 0.01). Conclusions All sesame seed extracts were categorized as very strong antioxidants by DPPH assay. Phenolic compounds in black sesame seeds were found to be the major contributors to antioxidant activities by using ABTS and FRAP methods. White and black sesame seeds have the potential to be developed as sources of natural antioxidants.

Objectives: The aims of this research were to determine antioxidant capacity of various extracts from black nightshade, turkey berry, and round green eggplant using five antioxidant assays which were ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), H 2 O 2 scavenging, phosphomolybdenum assay, and beta-carotene bleaching (BCB), correlation of total phenolic, flavonoid, and carotenoid content with their inhibitory concentration 50% (IC 50 ) and exhibitory concentration 50% (EC 50 ) of five antioxidant assay and correlation between five antioxidant assays. Methods:Extraction was conducted by reflux using gradient polarity solvents. The extracts were evaporated using rotary evaporator. The antioxidant capacity study, determination of phenolic, flavonoid, and carotenoid content were performed by ultraviolet-visible spectophotometry, while its correlation with IC 50 and EC 50 of five methods were analyzed by Pearson's method.Results: Ethanolic and ethyl acetate fruit extracts of turkey berry denoted the highest antioxidant capacity using FRAP (EC 50: 41.32 µg/ml), H 2 O 2 scavenging assay (IC 50: 1.01 µg/ml), and CUPRAC (EC 50: 117.56 µg/ml). While ethyl acetate fruit extract of round green eggplant gave the highest phosphomolybdenum capacity (EC 50 : 375.47 µg/ml), and ethyl acetate fruit extract of black nightshade showed the highest BCB capacity (EC 50: 158.66 µg/ml). Phenolic content of all fruit extracts had a tendency to correlate with FRAP and H 2 O 2 scavenging antioxidant capacity, meanwhile flavonoid and carotenoid content had a tendency to correlate with CUPRAC, phosphomolybdenum, and BCB antioxidant capacity.Conclusions: Phenolic compounds were a major contributor in antioxidant capacity of black nightshade, turkey berry, and round green eggplant extracts using FRAP and H 2 O 2 scavenging, meanwhile flavonoid and carotenoid compounds were a major contributor in antioxidant capacity using CUPRAC, phosphomolybdenum and BCB assays. FRAP assay had linear correlation with H 2 O 2 scavenging, meanwhile CUPRAC had linear correlation with phosphomolybdenum and BCB.