The aim of this study is to evaluate antioxidant activity of aqueous (Aq.E) and methanolic (Met.E) extracts of Zygophyllum album L. (Zygophyllaceae) and Arthrophytum scoparium (Chenpodiacea), two medicinal plant from Algerian Sahara used in traditional pharmacopoeia. The results showed that Aq.E have the heights yields 24% and 22.3% for Z. album and A. scoparium respectively. Qualitative analysis of showed that the Met.E of Z. album of had the highest amount of polyphenols (202.26±4.73 µg GAE/mg E) and flavonoids (15.77±0.94 µg QE/mg E). The antioxidant activity of extracts was evaluated by 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay. Results showed that the extracts of A. scoparium. Have the highest free radical scavenger activity in DPPH test (EC50=0.096±0.001 and 0.108±0.002 mg/ml for Met.E and Aq.E respectively). In addition, the results of reducing power assay demonstrated that Met.E of Z. album had the highest effect with 2399.65±12.31 µg ascorbic acid equivalent/mg E.Our results showed that the methanolic extract reported a considerable free radicals scavenging activity and reducing effect, may be due to their richness on polyphenols and flavonoids.
To date, no studies have investigated the phytochemical screening and biological activities of Cachrys libanotis L. traditionally used for the treatment of gout and rheumatism. Therefore, we aimed to investigate, for the first time, the phytochemical contents, the xanthine oxidoreductase inhibition, antibacterial, and antioxidant activities of extracts derived from the roots of C. libanotis L. Roots were submitted to extraction and fractionation using three different solvents sequentially, in the ascending order of polarity. Subsequently, XOR was purified from bovine milk and its inhibition was evaluated using xanthine/XOR system. The antioxidant activities were assessed employed superoxide scavenging, β- carotene bleaching, DPPH scavenging, ferrous iron chelation and FRAP assays). The antibacterial activity was tested by disc diffusion assay against 11 ATCC strains. Extraction and fractionation gave crude extract (CrE), hexane extract (HxE), chloroform extract (ChE), ethyle acetate extract (EAE) and aqueous extract (AqE). EAE exhibited the highest XOR inhibitory effect. CrE exhibited the highest effect as DPPH scavenger. All fractions exhibited a good activity against linoleate oxidation. Both CrE and AqE had an excellent chelating activity. Extracts showed a medium reducing power with greatest value exhibited by EAE. The plant extracts exhibited moderate to good antibacterial activity where the HxE extract had the strongest antimicrobial activity. From the obtained results, C. libanotis roots might be helpful in preventing or slowing the progress of gout, and it could be used as an additive in the food industry providing good protection against oxidative damage.
Keywords: Cachrys libanotis, polyphenols, oxidative stress, xanthine-oxidoreductase (XOR), antimicrobial.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.