Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice

Abstract

The objective of this study was to evaluate the effect of organic and conventional grape juices consumption on the behavior of rats and their neuroprotective effect on the activity of brain energy metabolism enzymes in different brain areas of adult rats on the experimental model of hepatic encephalopathy. Male Wistar rats (90-days-old) were treated once a day with conventional or organic white grape juice by gavage for 14 days (7 μL/g). On the 15th day the rats received carbon tetrachloride (CCl4) in a single dose of 3.0 mL/kg. Cerebral cortex, hippocampus and cerebellum were dissected to measure the activity of creatine kinase (CK) and pyruvate kinase (PK). No changes in feeding behavior were observed after the treatment with the grapes juices. However, there was an increase in grooming behavior in the open field test provoked by both juices. CCl4 inhibited CK activity in cerebral cortex and hippocampus of the rats and CCl4 also reduced PK activity in all brain structures studied. Furthermore, both white grape juices prevented the decrease in the activity of CK and PK. Therefore, we can suggest that organic and conventional white grape juices could restore the activity of enzymes with a central role in brain energy metabolism.

Effect of Chronic Treatment with Grape Juice during Pregnancy and Nursing in Central Nervous System of Female Wistar Rats

“…In our experiment, the animals treated with grape juice showed no significant difference between the groups. The same was found in other study of our group [33]. Our study is the first study to show this experimental design and this result.…”

Evaluation of physicochemical parameters of grape juices produced in the Serra Gaúcha

“…Rizzon and Miele [19] found values of 0.03 g/100g of acetic acid for whole juices, 0.05 g/100g of acetic acid for sweetened juices and 0.02 g/100g of acetic acid for the reprocessed juice, which are lower values than most found in this study. Gabardo et al [24] observed volatile acidity of 0.04 g/100g of acetic acid for both conventionally produced and organic white grape juice, those being low numbers when compared to the present work.…”

“…Canadian researchers [23] prepared juices from 10 different cultivars and found pH values of 2.38 to 3.13, displaying a lower number than the lowest observed in this study (2.81 for a white grape juice from the crop of 2013). Gabardo et al (2015) [24] evaluated the protective effect of white grape juice in rats and observed a pH of 3.89 in organic white grape juice and 3.58 in conventionally produced white grape juice, those being notably high values compared to the means of the present work.…”

Chronic purple grape juice consumption induces age-dependent changes on cognitive function in elderly women

“…In this context, polyphenol compounds found in grape juice have been associated with multiple health benefits including improvement in cognition and neuronal function with aging [21][22][23]. Experimental studies conducted by our research group demonstrated the neuroprotective effects of chronic purple grape juice consumption in several brain areas from rats submitted to different damage models [24][25][26].…”