Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Abstract

SUMMARYObjective: Pro-inflammatory cytokines, interleukin-6 (IL-6), demonstrated to be suppressed by interleukin-10 (IL-10) are known to be regulated by the transcription factor nuclear factor-κB(NF-κB). The aim of this study was to ascertain the association between genetic polymorphism of these genes (IL-6(-174), IL-10(-597) and NF-κB1-94ins/del)) and chronic/aggressive periodontitis.Methods: Forty-five patients with chronic periodontitis (CP), 58 patients with aggressive periodontitis (AP) and 38 periodontally healthy subjects were included in this study. Genomic DNA was isolated from whole blood samples. The NF-κB, IL-6, and IL-10 polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results: Among subjects for the ins/ins genotypes of NF-κB1 gene, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis group than in healthy controls (p = 0.023). A statistically significant difference in genotyping frequencies between AP group and healthy controls was observed for the IL-6 gene. The AA genotype of IL-10 was overrepresented in CP and AP groups compared to healthy controls (OR = 9.93, 95% CI: 2.11-46.7, OR = 5.7, 95% CI: 1.22-26.89, respectively).Conclusions: Within the limits of this study, it can be concluded that the IL-10 (-597) AA genotype is associated with susceptibility to chronic/ aggressive periodontitis and IL-6 (-174) GG genotypes and G allele seems to be associated with aggressive periodontitis.Clinical relevance: The results of the current study indicate that IL-6 and IL-10 genotypes seem to be associated with aggressive periodontitis. Also, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis subjects with carrying NF-κB1 ins/ins genotypes.

Interleukin‐10 gene polymorphisms and chronic periodontitis susceptibility: Evidence based on 33 studies

“…Among these studies, 25 studies investigated the ‐1082A>G(rs1800896) polymorphism, 15 studies investigated the ‐819C>T(rs1800871) polymorphism, and 18 studies investigated the ‐592C>A(rs1800872) polymorphism. Nine studies were performed in Asians, 17 in Caucasians, and seven in Latinos. Thirty‐two studies were conducted in systemically healthy subjects and one study in subjects with atherosclerotic and aortic aneurysmal vascular diseases (VD) (VD with CP and VD without CP).…”

“…Polymorphisms in cytokine genes often affect cytokine expression profiles . It has been reported that the ‐1082 G allele and GG genotype are associated with high production of IL‐10.…”

“…In the literature, data on which of the IL‐10 polymorphism contributes to periodontal disease are contradictory. Some of the studies failed to find any evidence of association, and others revealed a possible association between this polymorphism and periodontitis . It is important to note that the associations between the ‐819C>T(rs1800871) and ‐592C>A(rs1800872) polymorphisms and CP risk were suggested by two previous meta‐analyses …”

“…It is estimated that 50‐80% of the differences in the IL‐10 production can be explained by genetic factors . The gene encoding human IL‐10 is located on chromosome one (1q31‐32) . A large number of single nucleotide polymorphisms (SNPs) have been identified in the IL‐10 gene promoter.…”

“…A large number of single nucleotide polymorphisms (SNPs) have been identified in the IL‐10 gene promoter. Of interest are the polymorphisms at ‐1082 (also known as ‐1087), ‐819 (‐824), and ‐592 (‐597) . In the literature, data on which of the IL‐10 polymorphism contributes to periodontal disease are contradictory.…”