A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis

Abstract

We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses.

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

“…2C). This finding is consistent with the previous results that the accumulation of heat shock proteins is also found in plants under drought, salt, and oxidant stress (Kim et al 2012;Jacob et al 2017;Zhang et al 2018c;Zandalinas et al 2018). Actually, in addition to its protective effect under stress conditions, the heat shock protein also plays ; g and h, GSH; i and j, PARP; k and l, ATP; m and n, ATPase.…”

Preferential gene retention increases the robustness of cold regulation in Brassicaceae and other plants after polyploidization

“…Experimental evidence suggests that both CRGs and heat tolerance genes are often members of the temperature stress-responsive or thermostress-responsive (TSR) gene families. For example, the transgenic overexpression of a Primula forrestii heat-induced gene, PfHSP17.2, in Arabidopsis increased cold tolerance 44 . The overexpression of a zinc finger transcription factor (TaZnF) from wheat conferred tolerance to both heat and cold stresses in Arabidopsis 45 .…”

Over-Expression of the Heat-Responsive Wheat Gene TaHSP23.9 in Transgenic Arabidopsis Conferred Tolerance to Heat and Salt Stress

“…Therefore, we concluded that TaHSP23.9 plays a positive role in the HSR and acts as a chaperone to repair damage caused by heat stress. In addition, many studies have confirmed that sHSPs can be induced not only by heat stress but also by various stress environments, and OE of sHSPs in transgenic plants can enhance plant tolerance to multiple stresses (Zou et al, 2012;Mu et al, 2013;Zhang et al, 2014Zhang et al, , 2018Kuang et al, 2017). Therefore, we verified the phenotype of TaHSP23.9-OE lines and the expression FIGURE 5 | The relative expression level of TaHSP23.9 under salt stress, and the MDA, total soluble protein, and proline contents and root elongation of Col-0 (WT) and TaHSP23.9 over-expression transgenic lines under heat and salt tress.…”