Over-Expression of the Heat-Responsive Wheat Gene TaHSP23.9 in Transgenic Arabidopsis Conferred Tolerance to Heat and Salt Stress

Wang, Jun; Gao, Xin; Dong, Jun; Tian, Xinyu; Wang, Junzhe; Palta, Jairo A.; Xu, Shengbao; Fang, Yan; Wang, Zhonghua

doi:10.3389/fpls.2020.00243

Cited by 35 publications

(31 citation statements)

References 63 publications

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“…3B ). The transcript abundance of ClHSP22.8 in response to salt stress reached the lowest level in the first 4 h. The response pattern of ClHSP22.8 that rapidly and sharply responded to salt stress in a short time and then had slight variations, was similar to quite a few HSP20s such as TaHSP23.9 ( Wang et al, 2020 ), OsHSP20 ( Guo et al, 2020 ), AsHSP17 ( Sun et al, 2016 ) and so on. The response pattern of HSP20s probably is a kind of mechanism for plants rapidly adapt to salt stress.…”

Section: Discussionmentioning

confidence: 94%

“…As the largest family, and most well studied HSP, the HSP20 family of proteins is ATP-independent and generally assemble into large oligomers that can protect other proteins from denaturation and aggregation ( Papsdorf & Richter, 2014 ; Asea, Kaur & Calderwood, 2016 ). An increasing body of evidence has shown that HSP20s regulate the responses of plants to environmental changes and thus allow plants to survive adverse conditions ( Sun et al, 2012 , 2016 , 2020 ; Murakami et al, 2004 ; Charng et al, 2006 ; Shakeel, Heckathorn & Luthe, 2012 ; Khurana, Chauhan & Khurana, 2013 ; Zou et al, 2012 ; Kaur et al, 2015 ; Chauhan et al, 2012 ; Wang et al, 2020 ; Mu et al, 2013 ; Li et al, 2018 ). In a previous study, we identified HSP20 genes within the genome of watermelon and found that HSP20s in plants can be divided into 18 subfamilies ( He et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Promoter analyses further revealed that the over-expression of TaHSP26 in wheat could be induced by heat, cold, salt and drought ( Chauhan et al, 2012 ). Furthermore, the over-expression of TaHSP23.9 in wheat led to an enhancement in the tolerance to heat and salt stresses ( Wang et al, 2020 ). Another study, involving the ectopic expression of LimHSP16.45 led to an increase in the activities of superoxide dismutase (SOD) and catalase (CAT), thus improving the vigor of seed germination in Arabidopsis under salt stress ( Mu et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

“…As one of the most important stressors, salinity stress can lead to serious limitations in the yield and quality of watermelon ( Yetr & Uygur, 2009 ). HSP20s are the most abundant HSP sub-type in plants and are known to play important functions in a variety of stress responses ( Sun et al, 2012 , 2016 , 2020 ; Zou et al, 2012 ; Wang et al, 2020 ; Mu et al, 2013 ; Li et al, 2018 ). However, we know very little about the specific role of watermelon HSP20s with regards to abiotic stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

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A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways

Yao

et al. 2021

PeerJ

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Heat-shock protein 20s (HSP20) were initially shown to play a role during heat shock stress; however, recent data indicated that HSP20 proteins are also involved in abiotic stress in plants. Watermelon is known to be vulnerable to various stressors; however, HSP20 proteins have yet to be investigated and characterized in the watermelon. In a previous study, we identified a negative regulator of salt stress response from watermelon: ClHSP22.8, a member of the HSP20 family. Quantitative real-time PCR (qRT-PCR) and promoter::β-glucuronidase (GUS) analysis revealed that ClHSP22.8 was expressed widely in a range of different tissues from the watermelon, but particularly in the roots of 7-day-old seedlings and flowers. Furthermore, qRT-PCR and GUS staining showed that the expression of ClHSP22.8 was significantly repressed by exogenous abscisic acid (ABA) and salt stress. The over-expression of ClHSP22.8 in Arabidopsis lines resulted in hypersensitivity to ABA and reduced tolerance to salt stress. Furthermore, the expression patterns of key regulators associated with ABA-dependent and independent pathways, and other stress-responsive signaling pathways, were also repressed in transgenic lines that over-expressed ClHSP22.8. These results indicated that ClHSP22.8 is a negative regulator in plant response to salt stress and occurs via ABA-dependent and independent, and other stress-responsive signaling pathways.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways

Yao

et al. 2021

PeerJ

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“…Furthermore, HSPs, as molecular chaperones, are involved in folding, transferring and assembly of proteins, and they also can repair or remove denatured proteins in plant cell. HSP90, HSP70 and small HSPs (sHSPs) have been demonstrated to protect plants against the heat stress [14,15].…”

Section: Read Full License Introductionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

Wang

Liang

Yang

et al. 2020

Preprint

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Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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Genome‐wide identification and expression analysis of heat shock protein gene family in cassava

Wang,

Ran,

Zang

et al. 2023

The Plant Genome

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Heat shock proteins are important molecular chaperones that are involved in plant growth and stress responses. However, members of the Hsp family have been poorly studied in cassava. In this study, 225 MeHsp genes were identified in the cassava genome, and their genetic structures exhibited relatively conserved features within each subfamily. The 225 MeHsp genes showed random chromosomal distribution, and at least 74 pairs of segmentally duplicated MeHsp genes. Eleven tandemly duplicated MeHsp genes were identified. Cis‐element analysis revealed the importance of MeHsps in plant adaptations to the environment. The prediction of protein interactions suggested that MeHsp70‐20 may play a critical regulatory role in the interactive network. Furthermore, the expression profiles of MeHsps in different tissues and cell subsets were analyzed using bulk transcriptomics and single‐cell transcriptomic data. Several subfamily genes exhibited unique expression patterns in the transcriptome and were selected for detailed analysis of the single‐cell transcriptome. Quantitative real‐time polymerase chain reaction (qRT‐PCR) revealed the expression patterns of these genes under temperature stress, further supporting the prediction of cis‐acting elements. This study provides valuable information for understanding the functional characteristics of MeHsp genes and the evolutionary relationships between MeHsps.

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Over-Expression of the Heat-Responsive Wheat Gene TaHSP23.9 in Transgenic Arabidopsis Conferred Tolerance to Heat and Salt Stress

Cited by 35 publications

References 63 publications

A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways

A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways

iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

Genome‐wide identification and expression analysis of heat shock protein gene family in cassava

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