Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no íleo de frangos infectados por Toxoplasma gondii

Shiraishi, Celina Sayuri; Azevedo, Jorge Fernandes de; Silva, Aristeu Vieira da; Sant’Ana, Débora de Mello Gonçales; Araújo, Eduardo José de Almeida

doi:10.1590/s0103-84782009000700030

Cited by 20 publications

(12 citation statements)

References 22 publications

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“…Thus, it is suggested that the increase of these birds' secretion of mucin neutral would thicker the mucous covering the epithelium, protecting it from abrasion, what usually occurs during diarrhea. Similar result has also been observed when the ileum of birds in this study was assessed through the technique of detection of glycoconjugates (Shiraishi 2008). Studies on experimental infection with T. gondii demonstrated that the sporozoites cross the intestinal mucosa either through the enterocytes or the caliciform cells (Speer & Dubey 1998).…”

Section: Discussionsupporting

confidence: 85%

Effects of infection with Toxoplasma gondii oocysts on the intestinal wall and the myenteric plexus of chicken (Gallus gallus)

et al. 2010

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787Pesq. Vet. Bras. 30(9):787-792, setembro 2010 RESUMO.-[Efeitos da infecção por oocistos de Toxoplasma gondii sobre a parede intestinal e o plexo mientérico de Gallus gallus.] O objetivo deste trabalho foi analisar os efeitos da infecção pelo Toxoplasma gondii sobre a parede intestinal e o plexo mientérico de Gallus gallus. Dez galinhas de 36 dias de idade separadas em dois grupos: controle e experimental inoculado com oocistos da cepa M7741 de T. gondii (genótipo III) pela via oral. Após 60 dias os animais foram submetidos à eutanásia e o duodeno coletado. Parte dos segmentos intestinais foi submetida à rotina histológica, coloração por HE e técnica histoquímica de PAS e Alcian Blue. Realizou-se uma avaliação qualitativa da parede intestinal e medidas comparativas entre os grupos da espessura da parede total, túnica muscular, muscular da mucosa e túnica mucosa. As célu-las caliciformes foram quantificadas. Outra parte dos seg- This paper aims to analyze the effects of the Toxoplasma gondii infection in the intestinal wall and myenteric plexus of chicken (Gallus gallus). Ten 36-day-old chickens were separated into two groups: control and experimental, orally inoculated with oocysts of the T. gondii strain M7741 genotype III. After 60 days the birds were submitted to euthanasia and had their duodenum removed. Part of the intestinal segments was submitted to histological routine, HE staining, PAS histochemical technique, and Alcian Blue. Qualitative analysis of the intestinal wall and comparative measurements among the groups with respect to total wall thickness, muscle tunic, mucosa, and tunica mucosa were carried out. Caliciform cells were quantified. The other part of the intestinal segments was fixed in formol acetic acid and dissected having the tunica mucosa and the tela submucosa removed. Neurons were stained with Giemsa, counted, and measured. Chickens from the experimental group presented diarrhea and inflammatory infiltrates in the tunica mucosa, thickness reduction of all the parameters assessed in the intestinal wall, and an increase of the number of caliciform cells. There was a ~70% reduction regarding the intensity of myenteric neurons; and the remaining cells presented a reduction of ~2.4% of the perikarion and ~40.5% of the nucleus (p<0.05). Chronic infection induced by T. gondii oocysts resulted in intestinal wall atrophy, mucin secretion increase, death and atrophy of chicken myenteric plexus neurons. Death and atrophy of myenteric plexus neurons may be related with the causes of diarrhea observed in chickens with toxoplasmosis. Effects of infection withINDEX TERMS: Chicken, toxoplasmosis, duodenum, enteric nervous system, experimental infection. mentos intestinais foi fixada em formol acético e dissecada retirando-se a túnica mucosa e a tela submucosa. Os neurônios foram corados pela técnica de Giemsa, contados e mensurados. Os animais do grupo experimental apresentaram diarréia e infiltrados inflamatórios na túnica mucosa, redução da espessura de todos os parâmetros avaliados da parede in...

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Section: Discussionsupporting

confidence: 85%

Effects of infection with Toxoplasma gondii oocysts on the intestinal wall and the myenteric plexus of chicken (Gallus gallus)

et al. 2010

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“…When T. gondii crosses the gastrointestinal tract wall it can cause multifunctional alterations [31][32][33] . These alterations seem to be related to several factors such as genotype strain, life form and inoculation route (oral or intraperitoneal) of the parasite in addition to the infection phase (acute or chronic), digestive tract region and the type of cells assessed [5][6][7][8][9][10][11][12][13][14][15] .…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondiicauses death and plastic alteration in the jejunal myenteric plexus

Araújo

Zaniolo

Vicentino

et al. 2015

WJG

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“…Despite the many studies in literature on intestinal morphometry, most do not clearly describe the methodology applied. Relevant data, such as the evaluated intestinal segment, the time between death and sample fixation, how the segments are sectioned, and criteria to measure the microstructures, are not informed (Alvarenga et al, 2004;Fukayama et al;, Okamoto et al, 2009and Shiraishi et al, 2009. On the other hand, Caruso & Demonte (2005), studying the histomorphometry of the small intestine of rats, describe the details of the methodology applied and of the measurement criteria used.…”

Section: Introductionmentioning

confidence: 99%

Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers

Gava

Moraes

Carvalho

et al. 2015

Rev. Bras. Cienc. Avic.

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Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no íleo de frangos infectados por Toxoplasma gondii

Abstract: Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no íleo de frangos infectados por Toxoplasma gondii

Cited by 20 publications

References 22 publications

Effects of infection with Toxoplasma gondii oocysts on the intestinal wall and the myenteric plexus of chicken (Gallus gallus)

Effects of infection with Toxoplasma gondii oocysts on the intestinal wall and the myenteric plexus of chicken (Gallus gallus)

Toxoplasma gondiicauses death and plastic alteration in the jejunal myenteric plexus

Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers

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