Função osteoindutora de fragmentos ósseos conservados em glicerina a 98%: estudo experimental em ratos

Cavassani, Marluce Macedo; Moraes, Julieta Rodini Engrácia de; Filho, J.G. Padilha

doi:10.1590/s0103-84782001000300013

Cited by 6 publications

(8 citation statements)

References 9 publications

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“…Glycerin was a good conservation medium for those fragments intended for grafting because, besides being of low cost, it kept bone fragments free of contamination, reducing antigenicity and preserving the functions of osteoinduction and osteoconduction, as described by other authors (Cavassani et al 2001, Ziliotto et al 2003. Furthermore, none of the animals had signs of contamination, infection of the surgical wound or rejection related with the presence of the graft, demonstrated by the complete graft-bone incorporation observed early at 45 days in some animals.…”

Section: Discussionmentioning

confidence: 81%

Using allogeneic cortical graft preserved in glycerin as spacer in the advancement of tibial tuberosity in 34 dogs

et al. 2018

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Cranial cruciate ligament is the main responsible for knee stability by preventing cranial tibial displacement regarding the femur. Deficiency in this ligament (CCLD) may cause subluxation of the tibia and dysfunction of the pelvic member due to overloading. Tibial osteotomies are among the more current surgical techniques for treating CCLD in dogs and they proportionate the dynamic stability by means of modifying bone geometry and the distribution of forces acting on the articulation. The objective of this work is to describe the use of the allogeneic cortical bone graft conserved in glycerin as a spacer on the tibial tuberosity advancement (TTA) for treating the CCLD. In order to do that, 34 dogs submitted to TTA surgery correction were evaluated, being 23 males (67.35%) and 11 females (32.35%). Surgical procedures happened from May 2011 to October 2015. Regarding the surgical procedure after osteotomy of the tibial tuberosity, a disk of allogeneic cortical disk, sawn wedge-hapsed, conserved in glycerin, proportions of 2x1mm was applied as spacer, enabling TTA. Advancements from 3 to 12 mm were executed, depending on the need of the patient. For animals with patella dislocation, trochleoplasty and TTA were executed in order to correct the deviation. The mean ± SD age of animals was 6.67±3.58 and weight was 15.16±12.97 kg. Mongrel dogs, Poodles and Yorkshire terriers were the most affected ones. From the 36 evaluated knees, 11 (30.56%) were associated with some traumatic process and in 25 (69.44%) there was no relation with previous trauma. From those wounds, 20 (55.56%) happened in the right limb and 16 (44.44%) in the left limb and two animals had CCLD bilaterally. Animals had continuous support, discreet drawer movement and negative tibial compression 15 days after surgery. At 30 days, 26 cases (72.22%) had firm support (FS); at 45 days, 24 cases (66 test at 7 and 67%) had FS and eight cases (22.22%) without claudication (WC). During subsequent radiographic evaluations the progressive incorporation of the graft and osteotomy union were observed. In this study, most of the diagnosed CCLD occurred in males diverging from results obtained by other authors that found greater frequency in females. Support without claudication it was observed in most of the cases of implants at 60 days. We concluded that the conserved allogeneic cortical bone graft was able to promote bone union in TTA of dogs with CCLD. None of the animals had signs of contamination, infection of the surgical wound or rejection related with the presence of the graft, demonstrated by the complete graft-bone incorporation observed early at 45 days in some animals. The glycerin was a good conservation medium for those fragments intended for grafting because, besides being of low cost, it kept bone fragments free of contamination, reducing antigenicity and preserving the functions of osteoinduction and osteoconduction. The possibility of molding the graft to the animal need is a characteristic favorable to executing the modified technique that could be molded according to the size of the animal, allowing perfect adaptation to the osteotomized local in different breeds. Intercurrences commonly observed in TTA with patellar dislocation, meniscal lesions, tibial crest fracture and displacement were not found in the animals of this study, probably due to the better distribution of forces between the pass screw in TT and the TTA plate confirming that it has good adaptation to the technique conferring to the modified TTA advantages regarding the conventional TTA.

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Section: Discussionmentioning

confidence: 81%

Using allogeneic cortical graft preserved in glycerin as spacer in the advancement of tibial tuberosity in 34 dogs

et al. 2018

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“…The use of bone grafts is becoming frequent in orthopedics, as a method for resolution of comminuted fractures, (fractures in which the bone is splintered or crushed) thus significantly reducing the need to amputate an affected limb (Cavassini et al, 2001).…”

Section: Wwwintechopencommentioning

confidence: 99%

Technologies Applied to Stimulate Bone Regeneration

Santos¹,

Lombello²,

Genari³

2012

Tissue Regeneration - From Basic Biology to Clinical Application

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“…Costa (1996) testou a utilização de osso cortical conservado em glicerina na reparação de falhas ósseas em cães, relatando que o meio testado preservou as característi-cas osteoindutora e osteocondutora dos enxertos; porém, verificou que os mesmos tornaram-se menos resistentes. Cavassani et al (2001), também testaram a glicerina como meio de preservação de fragmentos ósseos de ratos. Ao final do experimento, os autores verificaram que os implantes realizados no tecido subcutâneo e intramuscular apresentaram resposta osteogênica positiva, concluindo que a glicerina foi um bom meio para a conservação de fragmentos ósseos para uso em enxertos, uma vez que a função osteoindutora foi preservada.…”

Section: Introductionunclassified

Variação da força de resistência à micro-tração de fragmentos de ossos corticais preservados em diversos meios e a fresco: estudo experimental em coelhos

et al. 2009

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345Pesq. Vet. Bras. 29(4): 345-352, abril 2009 RESUMO.-Devido ao crescente uso dos aloenxertos nas cirurgias ortopédicas, há a necessidade do conhecimento de suas características biomecânicas ao longo do tempo de preservação. O presente trabalho consistiu na aná-lise da força de resistência à micro-tração de amostras de ossos corticais de coelho preservadas em diversos meios por até 180 dias e a fresco. Os resultados revelaram que a resistência e o tempo de preservação apresentaram uma relação inversamente proporcional, significando que, quanto maior o tempo de preservação, menor a resistência física avaliada no ensaio biomecânico de resistência à micro-tração. Dos meios utilizados, a glicerina Due to the increasing use of cortical bone allografts in orthopedic surgeries, de knowledge of its biomechanics characteristics during preservation time is needed. The present study consisted in the analyses of the resistance power to the micro-traction of samples of rabbit cortical bones preserved in several means for up to 180 days and fresh. The results showed that the resistance and the preservation time presented an inversely proportional relation, meaning that, the longer the time of preservation, the shorter the physical resistance evaluated in the biomechanical rehearsal of resistance to the micro-traction. The glycerin has presented lower values in relation to the resistance test, showing, after 30 days, only 24.58% of the present power in the fresh bone, and by 180 days, 1.76%. The samples submitted to autoclavation also showed low values by the end of the experiment, while they remained with just 12.31% of the power present in the fresh bone. The bones preserved in homologous plasma, Dakin liquid and the ones cryopreserved showed the best levels of resistance at the end of the experiment, remaining, respectively with 82.47, 70.34 and 66.72% of the maximum power, while compared with the resistance of the fresh bones. The conclusion is that the choice of methodology and time of preservation interfered directly in the biomechanics of the cortical bones, promoting decrease of the resistance capacity to the traction along the period of preservation.

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Função osteoindutora de fragmentos ósseos conservados em glicerina a 98%: estudo experimental em ratos

Abstract: RESUMO O objetivo do presente trabalho foi o de avaliar a função osteoindutora atribuída aos fragmentos ósseos conservados em glicerina a 98%, por trinta dias

Cited by 6 publications

References 9 publications

Using allogeneic cortical graft preserved in glycerin as spacer in the advancement of tibial tuberosity in 34 dogs

Using allogeneic cortical graft preserved in glycerin as spacer in the advancement of tibial tuberosity in 34 dogs

Technologies Applied to Stimulate Bone Regeneration

Variação da força de resistência à micro-tração de fragmentos de ossos corticais preservados em diversos meios e a fresco: estudo experimental em coelhos

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