The findings in this study will be useful in the diagnosis of ocular diseases in Rusa unicolor.

No abstract

A Yellow-headed Caracara (Milvago chimachima) was submitted to the Ophthalmology Service of the Federal University of Bahia with a corneal abnormality. During ophthalmic evaluation the right cornea was stained positively with fluorescein; a blurred bullous lesion, with irregular surface, compatible with the diagnosis of bullous keratopathy was found. This is a rare condition in a bird which was treated successfully with a modified third eyelid flap associated with antibiotic and hyaluronic acid eye drops. The adopted therapeutic proved to be simple to implement and viable for repair of the bullous keratopathy in the Yellow-headed caracara.

Este trabalho objetivou determinar os microrganismos bacterianos no saco conjuntival de cães clinicamente normais e cães com ceratoconjuntivite seca (CCS), atendidos no Hospital de Medicina Veterinária da Universidade Federal da Bahia. Foram incluídos na pesquisa 30 animais sadios e 28 com CCS. Foi observado crescimento bacteriano em 60% das amostras oculares dos cães sadios e em 89,29% dos cães com CCS. Houve predomínio de espécies Gram positivas em ambos os grupos. Nos animais normais as bactérias mais prevalentes foram Staphylococcus intermedius (26,83%), seguido por Staphylococcus aureus (21,95%) e Staphylococcus epidermidis (19,51%). Houve identificação de leveduras em 38,34% das amostras. Dos 30 animais sadios estudados, 46,66% apresentaram cultura positiva bilateral, 26,67% cultura positiva unilateral e 26,67% cultura negativa. Nos animais com CCS, as bactérias de maior prevalência foram o Streptococcus β-hemolítico (16,13%), seguido por Staphylococcus intermedius (12,90%) e o Staphylococcus aureus (11,83%). Ocorreu identificação de leveduras em 19,64% das amostras. Dos 28 animais com CCS, observou-se que 82,14% apresentaram cultura positiva bilateral e 17,86% cultura positiva unilateral.

RESUMO.-[Produção lacrimal, pressão intraocular, microbiota, citologia e histologia conjuntival de coelhos Nova Zelândia.] O objetivo deste estudo foi estabelecer valores de referência para testes diagnósticos oftálmicos em coelhos da raça Nova Zelândia (Oryctolagus cuniculus). 22 coelhos, machos, adultos foram utilizados. Foi mensurada a produção lacrimal através do teste lacrimal de Shirmer 1 (TLS1) e da Tira endodôntica de papel absorvente (EA-PPTT) de duas marcas comerciais distintas. The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.INDEX TERMS: Tear production, intraocular pressure, conjunctival microbiota, New Zealand rabbits, Oryctolagus cuniculus, lagomorphs, ophthalmology, Schirmer tear test, tonometry, endodontic paper point. Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) histologia revelou epitélio estratificado caracterizado pela presença de células epiteliais colunares com grande núme-ro de células caliciformes. Os achados deste estudo poderão ser utilizados com fins terapêuticos ou experimentais.

Avaliação do polimetilmetacrilato como implante ocular em coelhos submetidos à evisceraçãoApós a remoção do volume ocular ou de seu conteúdo, o implante de esferas de diferentes tipos de materiais previne a concentração da cavidade orbital. O objetivo deste estudo foi avaliar a esfera de polimetilmetacrilato (PMMA), utilizado como implante intraocular em coelhos submetidos à evisceração. Doze coelhos da raça Nova Zelândia foram submetidos à evisceração unilateral do olho esquerdo, com posterior implantação da esfera de PMMA com 12 mm de diâmetro. A avaliação clínica foi realizada diariamente nos primeiros 15 dias após a cirurgia e em intervalos de 15 dias até o final do período de estudo (180 dias). Para análise histopatológica, três animais por período experimental foram submetidos à enucleação aos 15, 45, 90 e 180 dias após a evisceração. Não foi observada deiscência da ferida, sinais de infecção ou extrusão do implante em nenhum dos animais durante todo o período do estudo. O exame histológico revelou a formação de tecido fibrovascular ao redor dos implantes. O PMMA comportou-se como material inerte e não-integrável.Palavras-chave: prótese intraocular, enucleação, evisceração.

The purpose of this study was to evaluate the macroscopy and microstructure of a double setting alphatricalcium phosphate bone cement sphere provided with interconnection channels (alpha-TCP-i), as well as the integration of the implant with the rabbits' orbital tissue, through macroscopic analysis and histopathology. The external and internal surfaces of the alpha-TCP-i were evaluated macroscopically and by electron microscopy. Twelve New Zealand rabbits received 12mm implants of alpha-TCP-i following enucleation of the left eye. The clinical assessment was undertaken daily during the first 15 days, followed by fortnightly assessment until the end of the study period. For the morphological analysis, exenteration was performed in 3 animals per experimental period (15, 45, 90 and 180 days). The external and internal surfaces of the implant appeared solid, smooth and compact, with six channels which interconnected centrally. The micro-architecture was characterized by the formation of columns of hexagonal crystals. No signs of infection, exposure, dehiscence of sutures or extrusion of the implant were noted in any of the animals during the entire period of the study. The morphological evaluation demonstrated the presence of a thin capsule around the implant, from whence appeared fibro-vascular projections, which penetrated it through the interconnecting channels. In the first days after the insertion of the implant, an intense inflammatory reaction was noted. At 180 days, however, there were no signs of inflammation. The alpha-tricalcium phosphate cement implant was well tolerated in this rabbit model and appeared to be relatively inert with some fibrovascular ingrowth through the large channels.Keywords: implant, eye, prosthesis RESUMO Avaliaram-se a macroscopia e a microestrutura de esferas compostas por cimento ósseo de alfa-fosfato tricálcico de dupla pega munido de canais de interconexão (alfa-TCP-i) e a integração do implante ao tecido orbital de coelhos através de análise macroscópica e histopatológica. A superfície interna e externa do alfa-TCP-i foi avaliada macroscopicamente e por microscopia eletrônica. Após enucleação do bulbo ocular esquerdo, 12 coelhos da raça Nova Zelândia receberam implantes do alfa-TCP-i com 12mm de diâmetro. A avaliação clínica foi realizada diariamente nos primeiros 15 dias e depois a cada quinze dias até o fim do período de estudo. Para análise morfológica, seguiu-se a exenteração de três animais por período experimental (15, 45, 90 e 180 dias). As superfícies externa e interna do implante mostraram-se compactas

Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil.Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil.Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin.Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years.Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth.