Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Yamaguchi, Klenicy K. L.; Veiga, Valdir Florêncio da; Pedrosa, Tatiana do Nascimento; Vasconcellos, Marne Carvalho de; Lima, Émerson Silva

doi:10.1590/s0100-40422013000600015

Cited by 12 publications

(3 citation statements)

References 28 publications

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“…We sought, with this analysis; information in relation to the biological behavior of the oils, so that they can subsequently be used for in vivo studies. The cytotoxicity evaluation provides a security parameter in the compounds utilization having potential therapeutic activity (Yamaguchi et al, 2013). As for the cytotoxicity assay, the oils of cumin, anise and fennel were not cytotoxic in the same minimum inhibitory concentrations found for the tested yeast Such results suggest that these oils showed to be promising models in the selection of natural products with antifungal activity, to prevent and treat fungal infections in oral tissue.…”

Section: Discussionmentioning

confidence: 76%

Chemical composition of essential oils from the apiaceae family, cytotoxicity, and their antifungal activity in vitro against candida species from oral cavity

et al. 2019

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The aims of this research were: evaluate the chemical composition and the cytotoxicity of the Cuminum cyminum (cumin), Anethum graveolens (dill), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) essential oils, as well as their antifungal activity in vitro against ten Candida spp. isolates. The chemical composition of the oils was analyzed by means of gas chromatography coupled with mass spectrometry (GC/MS). The cytotoxicity assays were performed, using the cell proliferation reagent WST-1 in L929 mouse fibroblasts (20x10 3 well -1 ). The determinate the Minimum Inhibitory Concentration (MIC), was performed through the Broth Microdilution technique (CLSI). The chemical main components were the cuminaldehyde (32.66%) for cumin, carvone (34.89%) for the dill, trans-anethole (94.01%) for the anise and anethole (79.62%) for the fennel. Anise and fennel did not were cytotoxic in all the tested concentrations, however the cumin oil was cytotoxic in the concentration of 20 mg.mL -1 and the dill in the concentrations of 20 and 8 mg.mL -1 . All yeasts were susceptible against the evaluated essential oils. Cumin presented the lowest MIC against yeasts. We concluded that all the essential oils presented inhibitory action against Candida spp., and C. cyminum, P. anisum and F. vulgare were not cytotoxic in the same minimum inhibitory concentrations for the fungi. Composição química de óleos essenciais da família apiaceae, citotoxicidade e sua atividade antifúngica in vitro contra espécies de candida da cavidade oral ResumoOs objetivos desta pesquisa foram: avaliar a composição química e a citotoxicidade dos óleos essenciais de Cuminum cyminum (cominho), Anethum graveolens (endro), Pimpinella anisum (erva-doce) e Foeniculum vulgare (funcho), bem como sua atividade antifúngica in vitro contra dez isolados de Candida spp.. A composição química dos óleos foi analisada por meio de cromatografia gasosa acoplada à espectrometria de massa (GC / MS). Os ensaios de citotoxicidade foram realizados, utilizando o reagente de proliferação celular WST-1 em fibroblastos de ratinho L929 (20x103 poço-1). A determinação da Concentração Inibitória Mínima (MIC) foi realizada através da técnica de microdiluição em caldo (CLSI). Os principais componentes químicos foram o cuminaldeído (32.66%) para cominho, carvona (34.89%) para o endro, trans-anetol (94.01%) para erva-doce e anetol (79.62%) para a funcho. O endro e a erva-doce não foram citotóxicos em todas as concentrações testadas, no entanto, o óleo de cominho foi citotóxico na concentração de 20 mg.mL -1 e o endro nas concentrações de 20 e 8 mg.mL -1 . Todas as leveduras foram suscetíveis aos óleos essenciais avaliados. O cominho apresentou a menor CIM contra as leveduras. Concluímos que todos os óleos essenciais apresentaram ação inibidora contra Candida spp., e C. cyminum, P. anisum e F. vulgare não foram citotóxicos nas mesmas concentrações inibitórias mínimas para os fungos.Palavras-chave: Cuminum cyminum, Candidíase atrófica crônica, Candida spp., antifúngico.

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Section: Discussionmentioning

confidence: 76%

Chemical composition of essential oils from the apiaceae family, cytotoxicity, and their antifungal activity in vitro against candida species from oral cavity

et al. 2019

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“…This assay evaluates cell viability [113], and the sample analyzed caused the death of around 78 % of human hepatocarcinoma cells (Hep G2) at 100 mg/mL [30]. On the other hand, the EO of leaves and stems from Endlicheria citriodora, when evaluated through the Alamar Blue assay in three cell lines (murine fibroblast and melanoma, and gastric adenocarcinoma) showed low toxicity, since at 50 µg/ mL, the samples did not cause the death of 50 % of the cells [42].…”

Section: Toxic Activitymentioning

confidence: 94%

Chemical Composition and Biological Activities of Essential Oils in the Family Lauraceae: A Systematic Review of the Literature

et al. 2019

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The Lauraceae family is predominantly found in Asia and in the rainforests of the Americas, and consists mostly of aromatic trees. Being an essential oil producer, this family is used in the food, pharmaceutical, and cosmetic industries. This work presents a systematic review of the chemical composition and bioactivity of the essential oils from the Lauraceae family. Medline, Scielo, Web of Science, Lilacs, and Scopus were employed to identify articles published between 2000 and 2018, using “Lauraceae”, “essential oil”, and “biological activity” as key words. From 177 studies identified, 53 met the inclusion criteria. These studies indicated a predominance of the compounds β-caryophyllene and 1,8-cineole in Lauraceae species, and highlighted the antioxidant, antifungal, antibacterial, and anti-inflammatory activities. Essential oils extracted from this family thus have high potential for pharmacological applications.

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“…Natural products provide the basis for many anti-infectious therapies currently in use, including polyenes, echinocandins and flavonoids, three major classes of naturally antifungal drugs [9,10]. With this in mind, the Lauraceae botanical family, known for its commercial interest owing to its essential oils, includes approximately 50 genera and 2500 species, of which the Ocotea genus stands out [11]. Many reports in the literature have addressed the chemical composition of Ocotea oils that occur worldwide [12,13].…”

Section: Introductionmentioning

confidence: 99%

Ocotea glomerata (Nees) Mez Extract and Fractions: Chemical Characterization, Anti-Candida Activity and Related Mechanism of Action

et al. 2020

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Background: Opportunistic fungal infections are increasingly common, with Candida albicans being the most common etiological agent; however, in recent years, episodes of candidiasis caused by non-albicans Candida species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti-Candida activity of Ocotea glomerata extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed. Methods: Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests. Results: All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 μg/mL) on C. krusei. The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for C. krusei and C. albicans, respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against C. krusei. Methanol extract did not reveal effects by ergosterol and sorbitol assays; however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death. Conclusion: O. glomerata methanol extract may be viewed as a rich source of biomolecules with antifungal activity against Candida spp.

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Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Cited by 12 publications

References 28 publications

Chemical composition of essential oils from the apiaceae family, cytotoxicity, and their antifungal activity in vitro against candida species from oral cavity

Chemical composition of essential oils from the apiaceae family, cytotoxicity, and their antifungal activity in vitro against candida species from oral cavity

Chemical Composition and Biological Activities of Essential Oils in the Family Lauraceae: A Systematic Review of the Literature

Ocotea glomerata (Nees) Mez Extract and Fractions: Chemical Characterization, Anti-Candida Activity and Related Mechanism of Action

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