Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods

Abstract

The biochemical methodology was proven to be adequate, and manual morphometry was shown to have limitations that may interfere with the statistical significance of correlations for the estimate of fibrosis intensity in the human myocardium.

Generation of an Adequate Perfusion Network within Dense Collagen Hydrogels Using Thermoplastic Polymers as Sacrificial Matrix to Promote Cell Viability

“…Hence, strategies to combine a high collagen concentration with cell survival must be discovered. In vivo, extracellular matrices are composed of concentrated collagen (around 30 mg·mL −1 in the heart [ 11 ]), but the cells survive due to a very developed vascularization network. For instance, about 2500 capillaries per mm 2 can be found in the heart to ensure cell survival [ 12 ].…”

Differential collagen expression in kidney and heart during hypertension

“…The second most abundant collagen type is type III, which represents around 11 to 12 % of all collagen fi brils. Its main function is to provide resilience and elasticity to the heart wall (17,18). There is insuffi cient information about collagen type V in the literature, however its normal values are below 5 % of the total collagen mass in the cardiac wall and it is associated with collagen type I (19,20).…”

First Chemosensor for Selective Detection and Quantification of L-4-Hydroxyproline in Collagen and Other Bio Samples

“…While comparing with other techniques our chemosensor is thus much advanced as it can easily detect Hyp selectively in a particular experiment and could be pertinent in high-throughput screening in biological samples (performance comparison is shown in table S1). [15][16][17][18][19][20] In molecular recognition chemistry, the exploration of fluorescent probes [21][22][23][24][25][26] with enviable properties are significantly important due to their indispensable role in medicinal and biological applications. [27][28][29] The synthetic chemosensors containing optical-signaling chromophoric parts generally imply sensing of certain biomolecules selectively via covalent linking.…”

“…13,14 Thus, selective detection and accurate quantification of Hyp in collagen/biological samples in general and urine or serum in particular holds significant promise for providing predictive and diagnostic information. There are few published methods like stable isotope labeling and LC−MS based, 15 colorimetricbased, 16 biochemical and morphometric based, 17 homogenization technique, 18 gas chromatography/mass spectrometry, 19 and enzymatic based 20 methods that have been reported for the detection and estimation of Hyp. However, so far no published articles did emphasize any chemosensor which explains the detection and direct quantification of Hyp in collagen or in other biotic or abiotic samples.…”

“…While comparing with other techniques our chemosensor is thus much advanced, as it can easily detect Hyp selectively in a particular experiment and could be pertinent in high-throughput screening in biological samples (performance comparison is shown in Table S1). − …”