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A modified method based on the principle of density gradient centrifugation, using lymphocyte separating media was developed for neutrophils isolation from peripheral blood of bovines and humans. The advent of this method with 95% purity, 96% viability, retained morphology and function of neutrophils showed excellent strategy for cell separation.

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A total of 57 male pigs were randomly allocated at I wk of age to one of four treatments: castrated and implanted with estradiol-170; castrated and implanted with testosterone; castrated and not implanted; not castrated and not implanted. The pigs were weaned at 3 wk of age and raised under conventional commercial conditions to 2, 4 or 6 mo of age. Within age group, treatment had no effect (P>0.05) on average daily gain, feed efficiency, backfat thickness or the growth coefficients of individual organs relative to body weight. Weights, lengths and circumferences of limb bones (humerus, scapula, tibio-fibula, patella, femur and pelvis) did not differ significantly among treatments within age groups. However, the radio-ulnae were thicker (P < 0.05) in boars than in the other three groups. No significant treatment differences in the rate of radiosulfate incorporation into the tibial or radial growth plates or on the rate of linear bone growth during the last 16-26 d of the study were detected. It is concluded that neither estradiol-17B nor testosterone therapy of young castrated male pigs is likely to influence their growth or bone development up to 6 mo of age.

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ABSTRACT. The present study assessed the individual and combined effect of vitamin E and omega 3 against low pathogenic avian influenza (LPAI) virus in broiler birds. Day-old broiler birds (n=125) were equally divided into five groups (A, B, C, D & E). Group A was non infected negative control. While groups B, C, D and E were infected with low pathogenic avian influenza (H9N2) virus at 28 th day (2 HAunits/bird). Group B was given omega 3 at rate of 150mg/kg in feed. Group C was given vitamin E 200 IU/kg. Group D was offered vitamin E and omega 3. Group E was designated as positive control without supplementation. The results revealed significantly increased antibody titer, decreased heterophil lymphocyte ratio, improved feed conversion ratio and least histopathological lesions in birds given vitamin E alone followed by the group given combination of vitamin E and omega 3. These results concluded the positive impact of Vitamin E as compare to omega 3 alone and combined omega 3 with vitamin E against LPAI infection in broiler chickens. These findings can have useful field application in control of avian influenza.

Thirty-two cull cows aged from I to 13 yr were allocated to five age groups and fed individually for 82 days, then slaughtered. Half of the cows within each group were implanted with 36 mg of zeranol . Zeranol had no significant effect on average daily gain, feed efficiency or backfat thickness, but caused a decrease in radio-ulna mass (P:0.10) and length (P:0.03). Rate of bone growth at the distal end of the radio-ulna decreased with increasing age to 0 at 4-5 yr.