No abstract

No abstract

A modified method based on the principle of density gradient centrifugation, using lymphocyte separating media was developed for neutrophils isolation from peripheral blood of bovines and humans. The advent of this method with 95% purity, 96% viability, retained morphology and function of neutrophils showed excellent strategy for cell separation.

A total of 57 male pigs were randomly allocated at I wk of age to one of four treatments: castrated and implanted with estradiol-170; castrated and implanted with testosterone; castrated and not implanted; not castrated and not implanted. The pigs were weaned at 3 wk of age and raised under conventional commercial conditions to 2, 4 or 6 mo of age. Within age group, treatment had no effect (P>0.05) on average daily gain, feed efficiency, backfat thickness or the growth coefficients of individual organs relative to body weight. Weights, lengths and circumferences of limb bones (humerus, scapula, tibio-fibula, patella, femur and pelvis) did not differ significantly among treatments within age groups. However, the radio-ulnae were thicker (P < 0.05) in boars than in the other three groups. No significant treatment differences in the rate of radiosulfate incorporation into the tibial or radial growth plates or on the rate of linear bone growth during the last 16-26 d of the study were detected. It is concluded that neither estradiol-17B nor testosterone therapy of young castrated male pigs is likely to influence their growth or bone development up to 6 mo of age.

No abstract