Eosinophilia can occur during P. marneffei infection, and this finding might provide additional information on the activity of this intracellular parasite. In addition, GM detection might be useful for monitoring the effect of antifungal treatments; however, this theory requires more data for verification.
The purpose of the present study was to evaluate the newest status of patients diagnosed Burkitt lymphoma (BL), an aggressive lymphoma subset with a high cure rate. Furthermore, the study aimed to create prognostic nomograms to consider various prognostic factors and estimate patient survival, paving the way for clinical decision-making. A total of 4,600 patients diagnosed with BL between 1983 and 2015 were investigated, via data collected from the SEER database. The overall status of the patients was analyzed through several aspects, including incidence and survival analysis of the previous three decades using the log-rank test and the Kaplan-Meier method. In order to construct and validate the nomograms, the patient diagnosed during 2005–2015 were randomly assigned to the training cohort and validation cohort. Univariate and multivariate analyses were applied to identify independent factors that were further included in the nomograms, predicting 3- and 5-year overall survival (OS) and cancer-specific survival (CSS). The data of the training cohort were used for internal validation and validation cohort used to external validation. C-index and calibration plots were used to validate the nomograms, comparing predicted values with actual outcomes. The incidence of BL was gradually increased from 1984 and reached its peak in 2009, at a rate of 0.491 per 100,000 [95% confidence interval (CI), 0.412–0.581]. From 2009, the incidence slowly declined year by year and dropped to 0.280 per 100,000 (95% CI, 0.224–0.346). The OS and CSS rates of patients diagnosed between 2005 and 2015 were increased, in contrast with those of patients diagnosed from 1983–1993 and 1994–2004. A total of five variables, including age, race, chemotherapy, primary site and stage, proved to be the prognostic factors of BL and were used to construct the nomograms predicting 3- and 5-year OS and CSS. The internal and external calibration plots for the probability of 3- and 5-year OS and CSS were consistent between nomogram prediction and observed outcomes. The slow decline in incidence and the significantly improved cure rate make BL a disease that is no longer an urgent problem. Effective nomograms were developed to predict the OS and CSS of patients with BL.
Despite improvement in the long-term survival rate following pediatric acute myeloid leukemia (AML), the rate remains low, even with optimal treatment. The present study reports the long-term outcome of a small patient group treated with a single drug, high-dose chemotherapy (HDCT) with cytarabine, including consolidation and maintenance therapy. RT-PCR was conducted to assess 43 fusion genes, and after treatment, all cases have been followed up for 20 years (June 2002-December 2020). With an 80% 5-year survival rate, the results of this study highlight the possibility that pediatric AML can be reasonably effectively treated with relatively simple chemotherapy when necessary. HDCT is clinically safe, effective and relatively inexpensive. We propose that in the context of limited resources, HDCT should be considered as an alternative therapy for pediatric AML.
Purpose The expression of eukaryotic translation initiation factor 2 subunit 3 (EIF2S3) in patients with non-small cell lung and colorectal cancer is lower than that in healthy individuals. However, the functions of EIF2S3 remain unclear, and its study in leukemia has not been reported. The article aims to explore the role of EIF2S3 in AML (acute myeloid leukemia) and its underlying mechanism.Methods Reverse transcription-quantitative PCR was performed to evaluate the expression levels of EIF2S3, and its association with patient prognosis was determined. Inducible HEL-EIF2S3 and HL-60-EIF2S3 cell lines were established by retrovirus infection. Cellular proliferation and the cell cycle were analyzed using Cell Counting Kit 8 and ow cytometric analyses. Tumorigenic ability was evaluated using xenograft nude mouse model. Gene expression pro les were analyzed in HL-60-EIF2S3 cells by next-generation sequencing, and WB analysis was performed to detect the expression of related proteins. ResultsThe expression of EIF2S3 in patients with AML was lower than that experiencing CR (P=0.02). Furthermore, EIF2S3 overexpression inhibited cellular proliferation, halted G0/1 to S phase cell cycle progression and inhibited tumorigenicity (P=0.015). 479 differentially expressed genes were identi ed between HL60-EIF2S3 DOX (-) and HL60-EIF2S3 DOX (+) cells via NGS and several of them involved in MAPK/ERK signaling pathway. The phosphorylation levels of ERK decreased when EIF2S3 was overexpressed(P 0.050).Conclusion EIF2S3 overexpression may result in a decrease in cellular proliferation, cell cycle arrest and tumorigenic inhibition via the MAPK/ERK signaling pathway in AML cells.
Background: Eukaryotic translation initiation factor 2 subunit gamma (EIF2S3) is a component of eIF2 ,which functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. The preliminary research suggested that in multiple malignant carcinomas, the expression level of EIF2S3 between the normal tissue and malignant one is Significantly different.It indicated that EIF2S3 might play an important role in the occurrence and development of malignant tumors .The purpose of this study is to investigate the expression status of EIF2S3 and its prognostic significance in AML patients. Methods:Total RNA was extracted from bone marrow of 42 patients. The expression of EIF2S3 was examined by Realtime quantitative PCR. The relationship of EIF2S3 expression and other clinicopathological charactors and itsprognosis significance were statistically analyzed. Results:The relative expression of EIF2S3 was much higher in patients who had achieved CR (median value 0.088, range: 0.0179-0.238;) compared with newly diagnosed AML patients(median value 0.037, range: 0.003-0.203),p value was 0.020.Furthermore, the expression of EIF2S3 was markedly associated with Complete Remission (P = 0.012) and outcome(P=0.029). Patients with lower EIF2S3 expression had a relatively poor overall survival(OS) (P = 0.016) and short disease-free survival(DFS) (P = 0.001). Moreover, EIF2S3 expression was an independent prognostic factor for both OS (P = 0.001) and DFS (P <0.001). Conclusions:Patients who had achieved CR usually had a higher level of EIF2S3 expression compared with newly diagnosed AML patients. The over-expression of EIF2S3 is associated with good prognosis in AML patients, and was an independent prognostic factor for both OS and DFS. Disclosures No relevant conflicts of interest to declare.
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