Abstrak Pengetahuan, sikap dan tindakan yang baik oleh tenaga kesehatan seperti dokter dalam upaya strategi pencegahan thalassemia sangat diperlukan, karena dokter yang berperan sebagai penyampai informasi kepada masyarakat. Penelitian ini bertujuan untuk mengetahui tingkat pengetahuan, sikap dan tindakan mahasiswa kedokteran tentang pencegahan thalassemia. Penelitian dengan pendekatan cross sectional study ini melibatkan 96 mahasiswa angkatan tahun I s.d IV, di Fakultas Kedokteran, Universitas Muhammadiyah Semarang antara bulan April-Juni 2018. Analisis pengetahuan, sikap, tindakan terhadap thalasemia menggunakan kuesioner. Skor tiap angkatan dibandingkan dengan uji Kruskal wallis, hubungan antara skor sikap, tindakan dengan pengetahuan diuji dengan regresi linier sederhana. Sebanyak 42,7 % mahasiswa memiliki pengetahuan yang baik, mayoritas memiliki sikap yang positif (72,9%), tindakan yang baik (91,7%). Sebagian besar (70,8%) memiliki rencana untuk memeriksakan status karier thalassemia, namun lebih memilih menunda pemeriksaan sampai saat akan menikah (58,3%). Terdapat hubungan yang signifikan (P=0,023) antara sikap dengan pengetahuan dan antara tindakan dengan pengetahuan (p=0,039). Mahasiswa memiliki pengetahuan, sikap dan tindakan yang baik tentang pencegahan thalassemia serta tingkat pengetahuan mempengaruhi sikap dan tindakan mahasiswa.   Abstract Adequate knowledge, attitudes and practice by health workers such as doctors in thalassemia prevention strategies are needed, because doctors as informers to the public. This study aims to determine knowledge, attitudes and practice of medical students about thalassemia. This study was a cross sectional study involving 96 students from grade year I to IV, at the Faculty of Medicine, University of Muhammadiyah Semarang between April-June 2018. Analysis of knowledge, attitudes, practice against thalassemia used a questionnaire. The score of each grade was compared using Kruskal Wallis, relationship between attitude, practice with knowledge was analyzed with simple linear regression. A total of 42.7% of students had good knowledge, the majority had a positive attitude (72.9%), good practice (91.7%). Most (70.8%) had plans to check thalassemia career status, but prefered to postpone until get married (58.3%). There was a significant relationship (p = 0.023) between attitudes with knowledge and between practice with knowledge (p = 0.039). Students have good knowledge, attitudes and practice and level of knowledge influences their attitudes and practice.

BACKGROUND: The health data of Central Java, Indonesia showed that diabetes mellitus (DM) was the second most increasing non-communicable disease in the province. More than 20 genes have been reported to be associated with DM. Calpain-10 (CAPN10) polymorphism has been reported to be associated with Type 2 Diabetes Mellitus (T2DM). However, the association between CAPN10 single nucleotide polymorphism (SNP)-19 and T2DM among Javanese ethnics has never been reported. Therefore, this study was conducted to investigate the association.METHODS: After fasting for 8 hours, blood samples were drawn from veins of 107 T2DM and 107 non diabetic subjects. A half of the drawn blood was collected for identification of CAPN10 SNP-19, and another half for measuring triglycerides and fasting blood glucose (FBG). Identification of CAPN10 SNP-19 was performed with polymerase chain reaction (PCR) method, while measurement of triglycerides and FBG with colorimetric enzymatic method.RESULTS: The number of T2DM Javanese subjects with 2R/3R and 3R/3R CAPN10 SNP-19 genotypes was significantly higher than the number of T2DM Javanese subjects with 2R/2R genotype (p=0.002). When each number of 2R/3R and 3R/3R T2DM subjects was compared with the number of 2R/2R T2DM subjects, the number of 2R/3R T2DM subjects was significantly higher than the number of 2R/2R T2DM subjects (p=0.000).CONCLUSION: Javanese subjects with 2R/3R and 3R/3R CAPN10 SNP19 genotypes might have susceptibility of T2DM.KEYWORDS: Calpain-10, CAPN10, polymorphism, type2 diabetes mellitus, triglycerides, fasting blood glucose

BACKGOUNDInvasive prenatal diagnosis (PND) through amniocentesis and chorionic villus sampling (CVS) can detect Down syndrome. Pregnant women usually experience a variety of psychological responses associated with invasive PND. This study is intended to assess depression, anxiety and stress levels and the factors related to their psychological responses in pregnant women with invasive prenatal diagnosis of Down syndrome. METHODSA cross sectional study was conducted at Kandang Kerbau Women's and Children's Hospital, Singapore. The psychological responses of 70 women undergoing PND were assessed by Depression Anxiety Stress Scale 21 (DASS 21) questionnaire. A multiple linear regression analysis was used to analyze association between knowledge and perceived risk with psychological responses (CI 95% and significance value p<0.05). RESULTSMore than half of the participants had normal anxiety (55.7%), stress (72.8%), depression levels (65.8%). The results revealed significantly higher level of anxiety in women with gestational age >13 weeks who had pursued amniocentesis. Women with no previous children had higher levels of depression and stress. Women who pursued amniocentesis had significantly higher anxiety scores compared to women undergoing CVS (p=0.015). CONCLUSIONS

Abstract. Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of this work is to identify the potential effects of methanolic extracts from four species of the Pleurotus genus cultivated in Indonesia on nasopharynx cancer (Raji cell line), and to investigate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). This study investigates four species members of Pleurotus (Pleurotus ostreatus, P. cystidiosus, P. flabellatus, and P. pulmonarius var. stechangii). Dry Samples were extracted with methanol to yield crude extracts. Cytotoxicity screening was conducted using MTT assay of dry extracts, while antibacterial activity was calculated based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Mueller–Hinton broth, via the microdilution method. Compounds were analyzed using thin-layer chromatography (TLC). P. flabellatus provided the highest yield of dry extract (20.8%) with the lowest value of IC50 (556.226 µg/mL) compared to the three other species investigated. Antibacterial activity was calculated as MIC and MBC values against MRSA by the P. flabellatus extract which reached 6.25 mg/mL and 250 mg/mL, respectively. The result of TLC of the dry extract of P. flabellatus revealed the presence of terpenoids. P. flabellatus has the potential to be developed as both an anti-cancer and an antibacterial agent, especially against Raji cells and MRSA strains. However, further in vivo research and discovery of the modes of action involved are still needed to shed light on these effects. Studies can provide new information about the benefits of Pleurotus as a source of natural anticancer and antibacterial compound.

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BACKGROUND: One of the hormones that plays a role in glucose metabolism of Type 2 Diabetes Mellitus (T2DM) is melatonin. Its genetic variation is believed to play a significant role in the pathophysiology of obese and non-obese T2DM. The role of MTNR1B (melatonin receptor coding gene) and SLC2A1 (Glucose transporter 1/GLUT 1 transporter coding gene) on the risk of obese and non-obese T2DM patients is controversial. This study aims to analyze the association between the rs10830963 MTNR1B and rs841853 SLC2A1 polymorphism to the risk of Javanese obese T2DM.METHODS: This was a cross-sectional study that involved 107 Javanese T2DM patients from primary heath care in Semarang. Furthermore, obese T2DM was defined by a body mass index (BMI) more than 25 kg/m2. The genetic variations examined were rs10830963 MTNR1B and rs841853 SLC2A1 polymorphism by PCR-RFLP methods. Blood biochemistry parameters were also examined. Allele and genotype frequencies of rs10830963 MTNR1B and rs841853 SLC2A1 polymorphisms were analyzed using the x2 test with p≤0.05 and 95% CI.RESULTS: There was a significant association between rs10830963 MTNR1B polymorphisms in obese and nonobese T2DM (p=0.044) and the CG genotype increased the risk of obese T2DM. Furthermore, the allele and genotype frequency of rs841853 SLC2A1 polymorphism in both group had no significant difference, with a p=0.756 and p=0.802, respectively. There was also no significant difference in the biochemical parameters' in both groups of the two genetic variants studied.CONCLUSION: The rs10830963 MTNR1B polymorphism is associated with the risk of obesity in Javanese T2DM patients but not for the rs841853 SLC2A1 polymorphism.KEYWORDS: polymorphism, MTNR1B, SLC2A1, obese, diabetes mellitus

<p>Diabetes mellitus (DM) often give a macrovascular complication such as Peripheral arterial disease (PAD). Ankle-brachial index (ABI) is a sensitive and specific examination for PAD. Obesity can be a risk factor of PAD. Obesity can be classified by the BMI (Body Mass Index) classification. The purpose of this research is to analyze the relationship of BMI with ABI in Type 2 DM (T2DM) patients of Javanese ethnicity. This study was an observational analytic research with case-control design and used a purposive sampling technique. There were 40 case samples and 40 control samples. Data analysis used the Independent T-test and Chi-square test. There was a significant differences on age (p=0,000), METs (p-value=0,003), and ABI (p-value=0,002) between the two groups. In the DM group was found that most abnormal ABIs were found in the higher BMI, even though there was no significant correlation by the statistic (p-value=0,255). BMI contributed less to the ABI value of Javanese ethnic T2DM patients.</p>

Background: Urbanization has led to changes in human health with the inception of non-communicable diseases such as Diabetes mellitus (DM). This is generally due to the adaptation to unhealthy and sedentary lifestyles, with poor diet patterns. The purpose of this research is to identify the rural and urban differences in characteristics and lipid profile among the Javanese ethnic of Type 2 Diabetes patients. Method: This was an observational analytic study, with the cross-sectional approach used to obtain data from 102 T2DM patients in Gunungpati (rural), Pandanaran and Purwoyoso Health Centers (urban). The characteristics assessed included age, sex, BMI, blood pressure, blood sugar levels and lipid profile. The BMI was calculated by dividing body weight in kg by body height in meters squared, based on the Asia Pacific criteria. The blood pressure was measured with a mercury sphygmomanometer, then the levels of FPG and lipid profile were taken after the patients fasted for the previous 8 hours. Furthermore, the Mann Whitney U test was used to test the characteristics differences with a significance value of p ≤ 0.05.Results: There were significant differences between FPG control (p = 0.032) and HDL levels (p = 0.006). Meanwhile, characteristic data such as age (p = 0.214), gender (p = 0.790), BMI (p = 0.196), and blood pressure (p = 0.123) did not have significant differences. Furthermore, insignificant results were also obtained at various levels of FPG (p = 0.917), cholesterol (p = 0.436), triglycerides (p = 0.724), LDL (p = 0.188), and Chol / HDL ratio (p = 0.297). In urban areas, the average cholesterol levels (185.59 ± 52.59) , HDL (62.60 ± 16.69 mg / dl), LDL (201.16 ± 59, 36 mg / dl), were higher than those in the rural areas. Conclusion: In the urban group, FPG control was significantly worse than rural. Furthermore, cholesterol, HDL, LDL of Javanese T2DM patients in urban areas are higher than those in rural areas. However, only HDL is significantly different