Visualizing the progression of fatty liver disease in vivo is of great significance in biomedical research of fatty liver, but hindered by the lack of suitable approaches with deep penetration, high specificity, and real-time observability for fatty liver imaging. Herein, two bright NIR-I/NIR-II fluorophores with D-π-A-π-D structures are designed and synthesized for fatty liver tracking in vivo. Such fluorophores show an ultrahigh quantum yield of 49.2% and 17.3% at the emission maximum of 930 and 975 nm, respectively. Most interestingly, these two fluorophores display ultrasensitive fluorescence response (over 100-fold enhancement) toward the variations of environment polarity. Accordingly, further doping of these fluorophores into amphiphilic organic matrix allows the formation of ultrabright NIR-I/NIR-II fluorescent nanoprobes for high-resolution fluorescence imaging of the vessels. Additionally, with the assistance of such nanoprobes and NIR-II fluorescence imaging, real-time monitoring of heartbeat rate and the respiratory rate, accurate identification, and imaging-guided surgery of the submillimeter scale lymph nodes are successfully realized. Moreover, the as-designed fluorophores show superior performance for indicating lipid droplets (LDs) in living cells. Ultimately, precise monitoring of fatty liver with NIR-I/NIR-II fluorescent LDs probe is first confirmed, demonstrating the possibility for staging fatty liver disease with a contact-free method.

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The construction of multifunctional nano-enzymes is a feasible strategy for fighting multi-drug resistant (MDR) bacterial biofilm-associated infections. Extracellular DNA (eDNA) is an important functional part of biofilm formation, including the...

AIEdots with high brightness and narrow emission bandwidth were developed for multiplexed in vitro and in vivo fluorescence imaging.

An electrochemical-DNA (E-DNA) sensor was constructed by using DNA metallization to produce an electrochemical signal reporter in situ and hybridization chain reaction (HCR) as signal amplification strategy. The cyclic voltammetry (CV) technique was used to characterize the electrochemical solid-state Ag/AgCl process. Moreover, the enzyme cleavage technique was introduced to reduce background signals and further improve recognition accuracy. On the basis of these techniques, the as-prepared E-DNA sensor exhibited superior sensing performance for trace ctDNA analysis with a detection range of 0.5 fM to 10 pM and a detection limit of 7 aM. The proposed E-DNA sensor also displayed excellent selectivity, satisfied repeatability and stability, and had good recovery, all of which supports its potential applications for future clinical sample analysis.

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