Successful modern generalized gradient approximations (GGA's) are biased toward atomic energies. Restoration of the first-principles gradient expansion for exchange over a wide range of density gradients eliminates this bias. We introduce PBEsol, a revised Perdew-Burke-Ernzerhof GGA that improves equilibrium properties of densely-packed solids and their surfaces.
Fluorescent/phosphorescent dual-emissive conjugated polymer dots were designed and synthesized, ere used for tumor hypoxia sensing via ratiometric imaging and photoluminescence lifetime imaging.
An
outbreak of coronavirus disease (COVID-19) caused by severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses great
threats to human health and the international economy. To reduce large-scale
infection and transmission risk of SARS-CoV-2, a simple, rapid, and
sensitive serological diagnostic method is urgently needed. Herein,
an aggregation-induced emission (AIE) nanoparticle (AIE810NP, λem = 810 nm)-labeled lateral flow immunoassay
was designed for early detection of immunoglobulin M (IgM) and immunoglobulin
G (IgG) against SARS-CoV-2 in clinical serum samples. Using a near-infrared
(NIR) AIE nanoparticle as the fluorescent reporter (△λ
= 145 nm), the autofluorescence from the nitrocellulose membrane and
biosample and the excitation background noise were effectively eliminated.
After optimization, the limit of detection of IgM and IgG is 0.236
and 0.125 μg mL–1, respectively, commensurate
with that of the enzyme-linked immunosorbent assay (ELISA) (0.040
and 0.039 μg mL–1). The sensitivity of the
proposed AIE810NP-based test strip for detecting IgM and
IgG is 78 and 95% (172 serum samples), commensurate with that of ELISA
(85 and 95%) and better than that of a commercial colloidal gold nanoparticle
(AuNP)-based test strip (41 and 85%). Importantly, the time of detecting
IgM or IgG with an AIE810NP-based test strip in sequential
clinical samples is 1–7 days after symptom onset, which is
significantly earlier than that with a AuNP-based test strip (8–15
days). Therefore, the NIR-emissive AIE nanoparticle-labeled lateral
flow immunoassay holds great potential for early detection of IgM
and IgG in a seroconversion window period.
We have grown epitaxial thin films of spinel NiCo2O4 on single crystalline MgAl2O4 (001) substrates by pulsed laser deposition. Magnetization measurement revealed hysteresis loops consistent with the reported ferrimagnetic order. The electrical transport exhibits a metallic behavior with the lowest resistivity of 0.8 mΩ cm and a metal insulator transition around the Néel temperature. The systematic variation in the properties of the films grown at different growth temperatures indicates a close relationship between the magnetic order and electrical transport.
Molecular oxygen (O2) plays a key role in many physiological processes, and becomes a toxicant to kill cells when excited to 1O2. Intracellular O2 levels, or the degree of hypoxia, are always viewed as an indicator of cancers. Due to the highly efficient cancer therapy ability and low side effect, photodynamic therapy (PDT) becomes one of the most promising treatments for cancers. Herein, an early‐stage diagnosis and therapy system is reported based on the phosphorescent conjugated polymer dots (Pdots) containing Pt(II) porphyrin as an oxygen‐responsive phosphorescent group and 1O2 photosensitizer. Intracellular hypoxia detection has been investigated. Results show that cells treated with Pdots display longer lifetimes under hypoxic conditions, and time‐resolved luminescence images exhibit a higher signal‐to‐noise ratio after gating off the short‐lived background fluorescence. Quantification of O2 is realized by the ratiometric emission intensity of phosphorescence/fluorescence and the lifetime of phosphorescence. Additionally, the PDT efficiency of Pdots is estimated by flow cytometry, MTT cell viability assay, and in situ imaging of PDT induced cell death. Interestingly, Pdots exhibit a high PDT efficiency and would be promising in clinical applications.
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