The warm needling technique used in traditional Chinese medicine has been shown to be effective in the treatment and prevention of knee osteoarthritis (OA), but the biological mechanisms behind this action have not been well explored. This study investigated the molecular mechanisms behind warm needling using cDNA microarray technology, thus providing further scientific evidence for its efficacy. Ten patients with knee OA of deficiency-cold syndrome were selected for 2 weeks of warm needling treatment. This treatment involved stimulating the selected acupoints using needles with a burning moxa stick on their handle for 40 minutes per session. Pain intensity and accumulated clinical scores of deficiency-cold syndrome were assessed pretreatment and posttreatment using a 40-factor questionnaire of OA with deficiency-cold syndrome. Four patients with the best therapeutic efficacy were selected for cDNA microarray testing. Among the four patients, 41, 246, 57 and 70 differentially expressed genes were obtained, with more than 50% of these differentially expressed genes functionally linked to primary, cellular and energy metabolism pathways. This work demonstrates that the molecular mechanism behind warm needling treatment may be associated with the regulation of metabolism-related genes and pathways.
Objectives
Compared with nipple sparing mastectomy (NSM), microwave ablation (MWA) is one relatively new modality indicated for selected breast cancer with nipple sparing and with little of evidence-based medical research for decision-making. The objective of this study was to compare the effect of ultrasound-guided percutaneous MWA and NSM for breast cancer.
Materials and Methods
A retrospective cohort study was conducted in a single institution from 2014 to 2020. Women with invasive ductal carcinoma of the breast ≤ 5cm treated by MWA or NSM were enrolled. The primary end point was tumor progression and secondary end points included survival, cosmetic results, and complications.
Results
21 patients in the MWA group and 43 in the NSM group were evaluated. The mean tumor size was 2.3 cm (range, 0.3–5.0 cm). Median follow-up was 26.7 months (range, 14.6–62.5 months). The mean age of MWA was 24 years older than that of the NSM group. All the patients achieved technique effectiveness. One local tumor progression and one ipsilateral breast recurrence occurred at 42 and 28 months after MWA, respectively. One ipsilateral breast recurrence and two bone metastasis occurred at 31.2, 34, and 30.5 months after NSM. Two groups had no significant difference in tumor progression (P = 0.16). No participants in both groups developed cancer related death (P > 0.99) and major complications (P > 0.99). However, MWA needed less hospitalization time (P < 0.001) and achieved better cosmetic results (P < 0.001).
Conclusions
MWA achieved similar short term effect for breast cancer control and better cosmetic satisfaction compared with NSM in selected patients. MWA provides appropriate option for elderly patients who are unfit for surgery.
A total of 82 differentially expressed genes were identified, among which 27 were up-regulated and 55 down-regulated. Gene microarray assay is effective to identify differentially expressed genes and may find out novel osteoarthritis associated genes. Multiple genes are involved in the pathogenesis of osteoarthritis. The differentially expressed genes provide important information for further studies on the pathogenesis of osteoarthritis and gene therapy of osteoarthritis.
Objective: to observe the clinical effect of Wumei Pill combined with pilverbromonium tablets in the treatment of diarrhea irritable bowel syndrome. Methods: from January 2020 to December 2020, 60 patients with diarrhea irritable bowel syndrome were diagnosed. According to the random number table method, the patients were randomly divided into control group and trial group 30 cases. Results: (1) after treatment, the symptom score of the two groups was decreased compared with that before treatment (P <0.05); (2) after treatment, the symptom score of the experimental group was significantly lower than that of the control group (P <0.05); (3) the total efficiency of the experimental group was 86.67 and the control group was 63.33. The difference was statistically significant (P <0.05). Conclusion: The treatment of diarrhea irritable bowel syndrome with Wumei Pill combined with pilverbromonium tablets can effectively improve the symptoms and improve the clinical efficacy.
Objective: To observe the effect of Jiawei Shengjiyuhong Ointment on wound repair of rat model after anal fistula surgery by regulating NF-κBp65 / IL-1β / VEGF signaling pathway. Methods : Sixty rats were divided into vaseline model group (n = 10), + Kangfuxin fluid control group (n = 10), modified Shengji Yuhong Ointment group (n = 10), and PDTC intervention model group (n = 30) (PDTC ++ Kangfuxinfluid group, PDTC + vaseline group, PDTC + modified Shengji Yuhong Ointment group, Ten rats in each group). The wound model of rats after anal fistula operation was established and intervened in different ways. The general situation and wound healing of rats in each group were recorded, and the wound healing rate of rats in each group was calculated. On the 3 th, 7 th and 14 th day, two rats in each group were randomly selected and sacrificed. The wound tissues of the same site were taken for Western blotting and immunohistochemistry to detect the protein and expression of NF-κBp65 in the wound tissues. The expression of IL-1β / VEGF was detected by elisa method. At the same time, HE staining was used to observe the histomorphology.Results : Compared with the model control group, the wound healing rate of the modified Shengji Yuhong ointment group and PDTC + modified Shengji Yuhong ointment group was the highest. HE staining results also confirmed that the wound healing degree of the modified Shengji Yuhong Ointment group and PDTC + modified Shengji Yuhong Ointment group was the best. The results of Western blotting and immunohistochemistry showed that the protein expression of NF-κBp65 in the wound tissue of rats was significantly inhibited in the Jiawei Shengji Yuhong Ointment group, PDTC + Jiawei Shengji Yuhong Ointment group and PDTC++ Kangfuxin fluid group and PDTC + Jiawei Shengji Yuhong Ointment had the best effect, and the difference was statistically significant compared with the control group and the model group (P < 0.05). The elisa method showed that the content of IL-1β in Jiawei Shengji Yuhong Ointment was lower than that in the control group and the model group (P < 0.05) at 3 day and 7 day after administration, and it showed a gradually decreasing trend. The content of IL-1β in PDTC + Jiawei Shengji Yuhong Ointment was the lowest. The content of VEGF in Jiawei Shengji Yuhong Ointment was higher than that in the control group and the model group (P < 0.05), and it showed a gradually increasing trend. The content of VEGF in PDTC + Jiawei Shengji Yuhong Ointment was the highest.Conclusion : Jiawei Shengji Yuhong Ointment can inhibit the expression of NF-κB p65 and IL-1β protein in NF-κB signaling pathway, up-regulate the expression of VEGF protein, promote epithelial cell proliferation, reduce inflammatory response, restore normal dermal fiber tissue, accelerate the recovery of wound tissue, and shorten the healing time.
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