CircTP53 promotes colorectal cancer by acting as a miR-876-3p sponge to increase cyclin-dependent kinase-like 3 expression

Su, Yan; Wei, Hailiang; Li, Qian; Si, Mingming; Feng, Wenzhe; Chen, Zhiguo

doi:10.1016/j.cellsig.2020.109845

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…Mechanistically, circ-RNF111 was identified to promote KLF12 expression by serving as the sponge for miR-876-3p in GC cells. MiR-876-3p has been demonstrated to be targeted by circ_0088494 and circTP53 [ 29 , 30 ], but the association between circ-RNF111 and miR-876-3p was the first to be elucidated. Herein, miR-876-3p was reduced in GC patients MiR-876-3p overexpression repressed GC cell growth, metastasis, and glycolysis and accelerated apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12

Zhang

Yang

et al. 2021

World J Surg Onc

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Background The aberrant expression of circular RNAs (circRNAs) plays vital roles in the advancement of human cancers, including gastric cancer (GC). In this study, the functions of circRNA ring finger protein 111 (circ-RNF111) in GC were investigated. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circ-RNF111, microRNA-876-3p (miR-876-3p) and krueppel-like factor 12 (KLF12) mRNA. RNase R assay was conducted for the feature of circ-RNF111. Cell Counting Kit-8 (CCK-8) assay, colony formation assay, wound-healing assay, and transwell assay were applied for cell viability, colony formation, migration, and invasion, respectively. Flow cytometry analysis was used to analyze cell apoptosis and cell cycle process. The glycolysis level was examined using specific commercial kits. Western blot assay was carried out to measure the protein levels of hexokinase 2 (HK-2) and KLF12. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to verify the combination between miR-876-3p and circ-RNF111 or KLF12. Murine xenograft model was constructed for the role of circ-RNF111 in vivo. Immunohistochemistry (IHC) was used for KLF12 level. Results Circ-RNF111 was higher expressed in GC tissues and cells than normal tissues and cells. Silencing of circ-RNF111 restrained cell viability, colony formation, migration, invasion, cell cycle process and glycolysis and induced apoptosis in GC cells in vitro. Circ-RNF111 positively regulated KLF12 expression via absorbing miR-876-3p. MiR-876-3p downregulation reversed the impacts of circ-RNF111 silencing on GC cell malignant phenotypes. MiR-876-3p overexpression repressed GC cell growth, metastasis and glycolysis, inhibited apoptosis and arrested cell cycle, while KLF12 elevation weakened the effects. Besides, circ-RNF111 knockdown inhibited tumor growth in vivo. Conclusion Circ-RNF111 knockdown relieved the development of GC by regulating miR-876-3p/KLF12 axis.

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Section: Discussionmentioning

confidence: 99%

Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12

Zhang

Yang

et al. 2021

World J Surg Onc

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“…. Circ TP53 (Figure 5) was up-regulated in CRC tissues (163). It promoted proliferation, invasion, migration and reduced the apoptotic rate of CRC cells in vitro.…”

Section: Circ Tumor Protein 53 (Circtp53) Up-regulates Cyclindependen...mentioning

confidence: 92%

Up-regulated Circular RNAs in Colorectal Cancer: New Entities for Therapy and Tools for Identification of Therapeutic Targets

Weidle

Nopora

2023

Cancer Genomics Proteomics

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“…However, circHIPK3 was also reported to be downregulated in bladder cancer and involved in cell growth and metastasis inhibition, suggesting that circRNA function could be highly context-dependent, rendering tricky to define a particular circRNA as oncogenic or tumor suppressive [3,50]. Interestingly, circRNAs have been also shown to derive from master oncogenic or tumor suppressive genes, including circTP53, which was reported to promote colorectal cancer by sponging miR-876-3p and subsequently increasing cyclindependent kinase-like 3 expression [51]. An exome capture RNA sequencing project was recently conducted in more than 2000 cancer samples, including cell lines, tumors from diverse organs and non-pathological tissues.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“… 3 , 50 Interestingly, circRNAs have also been shown to derive from master oncogenic or tumour suppressive genes, including circTP53, which was reported to promote colorectal cancer by sponging miR-876-3p and subsequently increasing cyclin-dependent kinase-like 3 expression. 51 An exome capture RNA-sequencing project was recently conducted in more than 2,000 cancer samples, including cell lines, tumours from diverse organs and non-pathological tissues. Data were compiled into MiOncoCirc, the first international cancer-specific circRNA database.…”

Section: Overview Of Circrnas In Cancer: Mechanisms and Clinical Relevancementioning

confidence: 99%

Emerging roles of circular RNAs in liver cancer

2022

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CircTP53 promotes colorectal cancer by acting as a miR-876-3p sponge to increase cyclin-dependent kinase-like 3 expression

Cited by 13 publications

References 24 publications

Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12

Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12

Up-regulated Circular RNAs in Colorectal Cancer: New Entities for Therapy and Tools for Identification of Therapeutic Targets

Emerging roles of circular RNAs in liver cancer

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