Study on anti-inflammatory and healing mechanism of Jiawei Shengji Yuhong ointment on wound based on NF-κBp65 / IL-1β / VEGF pathway

Abstract

Objective: To observe the effect of Jiawei Shengjiyuhong Ointment on wound repair of rat model after anal fistula surgery by regulating NF-κBp65 / IL-1β / VEGF signaling pathway. Methods : Sixty rats were divided into vaseline model group (n = 10), + Kangfuxin fluid control group (n = 10), modified Shengji Yuhong Ointment group (n = 10), and PDTC intervention model group (n = 30) (PDTC ++ Kangfuxinfluid group, PDTC + vaseline group, PDTC + modified Shengji Yuhong Ointment group, Ten rats in each group). The wound model of rats after anal fistula operation was established and intervened in different ways. The general situation and wound healing of rats in each group were recorded, and the wound healing rate of rats in each group was calculated. On the 3 th, 7 th and 14 th day, two rats in each group were randomly selected and sacrificed. The wound tissues of the same site were taken for Western blotting and immunohistochemistry to detect the protein and expression of NF-κBp65 in the wound tissues. The expression of IL-1β / VEGF was detected by elisa method. At the same time, HE staining was used to observe the histomorphology.Results : Compared with the model control group, the wound healing rate of the modified Shengji Yuhong ointment group and PDTC + modified Shengji Yuhong ointment group was the highest. HE staining results also confirmed that the wound healing degree of the modified Shengji Yuhong Ointment group and PDTC + modified Shengji Yuhong Ointment group was the best. The results of Western blotting and immunohistochemistry showed that the protein expression of NF-κBp65 in the wound tissue of rats was significantly inhibited in the Jiawei Shengji Yuhong Ointment group, PDTC + Jiawei Shengji Yuhong Ointment group and PDTC++ Kangfuxin fluid group and PDTC + Jiawei Shengji Yuhong Ointment had the best effect, and the difference was statistically significant compared with the control group and the model group (P < 0.05). The elisa method showed that the content of IL-1β in Jiawei Shengji Yuhong Ointment was lower than that in the control group and the model group (P < 0.05) at 3 day and 7 day after administration, and it showed a gradually decreasing trend. The content of IL-1β in PDTC + Jiawei Shengji Yuhong Ointment was the lowest. The content of VEGF in Jiawei Shengji Yuhong Ointment was higher than that in the control group and the model group (P < 0.05), and it showed a gradually increasing trend. The content of VEGF in PDTC + Jiawei Shengji Yuhong Ointment was the highest.Conclusion : Jiawei Shengji Yuhong Ointment can inhibit the expression of NF-κB p65 and IL-1β protein in NF-κB signaling pathway, up-regulate the expression of VEGF protein, promote epithelial cell proliferation, reduce inflammatory response, restore normal dermal fiber tissue, accelerate the recovery of wound tissue, and shorten the healing time.