Talinum paniculatum is an edible plant with enormous medicinal properties. The main focus of this study was on callus induction due to the natural ability of callus that can regenerate into a new plant. This study was carried out to determine the effects of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction of T. paniculatum from leaf explant. Leaf explants of T. paniculatum were cultured on Murashige & Skoog (MS) media containing 2,4-D at concentrations of 1.0, 2.0, 3.0, 4.0 and 5.0 mg/L and BAP at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg/L under single exposure or combined exposure. Explants cultured on medium containing no plant growth regulators (PGR) served as control in this experiment. The effectiveness of PGRs on callus induction of T. paniculatum was determined by recording the callus induction day, percentage of callus induced in all the treatments and morphology of the callus induced. Leaf explants treated with 2,4-D and BAP in combined exposure resulted in callus induction. Highest percentage of callus from leaf explants was produced on the medium containing 2.0 mg/L of 2,4-D and 2.5 mg/L of BAP in combined exposure. In conclusion, callus induction of T. paniculatum could serve as an alternative regeneration pathway for this important medicinal pant.
An efficient protocol has been established for the regeneration of Cardamine hirsuta. The objective of this study was to evaluate the effect of types and concentrations of cytokinins on in vitro regeneration of C. hirsuta, an important medicinal and edible plant. Nodal explants of C. hirsuta were cultured in Murashige & Skoog (MS) media containing kinetin or zeatin singly at concentrations of 1.0, 2.0, 3.0, 4.0, and 5.0 mg /L. MS medium devoid of plant growth regulators served as control. The growth parameter observed including number of shoots, leaves, and roots. 2.0 mg/L zeatin induced the highest number of shoots and leaves. 1.0 mg/L kinetin-treated explants showed the highest formation of roots. From the results obtained, it was learnt that 2.0 mg/L zeatin was a more effective cytokinin compared to kinetin for in vitro regeneration of C. hirsuta as it induced the highest shoot and leaf growth while minimizing root formation. Concentrations of zeatin above optimal level not only caused stunted growth, but also resulted in hyperhydration. In conclusion, in vitro regeneration can serve as an alternative propagation method for C. hirsuta.
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